Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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Effect of Diluents and Temperature on Sperm Storage in Starry Flounder (Platichthys stellatus)

희석액과 보존온도에 따른 강도다리 (Platichthys stellatus) 정자의 냉장보존 효과

  • Lim, Han-Kyu (Aquaculture Research Team, National Fisheries Research and Development Institute) ;
  • An, Cheul-Min (Aquaculture Research Team, National Fisheries Research and Development Institute) ;
  • Son, Maeng-Hyun (Aquaculture Research Team, National Fisheries Research and Development Institute) ;
  • Park, Min-Woo (Aquaculture Research Team, National Fisheries Research and Development Institute) ;
  • Kim, Eung-Oh (Aquaculture Research Team, National Fisheries Research and Development Institute) ;
  • Byun, Soon-Gyu (Finfish Research Center, National Fisheries Research and Development Institute)
  • 임한규 (국립수산과학원 양식연구팀) ;
  • 안철민 (국립수산과학원 양식연구팀) ;
  • 손맹현 (국립수산과학원 양식연구팀) ;
  • 박민우 (국립수산과학원 양식연구팀) ;
  • 김응오 (국립수산과학원 양식연구팀) ;
  • 변순규 (국립수산과학원 어류연구센터)
  • Published : 2006.02.25
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Abstract

Experiments were performed to find out the physico-chemical properties of milt and sperm motility in various storage conditions using starry flounder (Platichthys stellatus). The average sperm concentration and spermatocrit in stripped milt were $6.00{\pm}0.98{\times}10^8/mL\;and\;72{\pm}5$, respectively. The osmolality and pH were $337{\pm}9mmol/kg$ and $7.7{\pm}0.1$, respectively. The sperm of starry flounder was preserved with artificial seminal plasma (ASP), Stein's solution (SS) and marine fish Ringer's solution (MFRS) at $0^{\circ}C,\;2^{\circ}C,\;and\;4^{\circ}C$. The most effective condition for cold storage was SS at $0^{\circ}C$, and the preserved sperm remained motile for 30 days.

다양한 희석액과 보존온도에 따른 강도다리 (Platichthys stellatus) 정자의 냉장보존 효과가 검토되었다. 강도다리 어미로부터 채정된 정액의 spermatocrit와 정자농도는 각각 $72{\pm}5$와 $6.00{\pm}0.98{\times}10^8/mL$였으며, 정장의 삼투질농도와 pH는 각자 $337{\pm}g\;mmol/kg$과 $7.7{\pm}0.1$이었다. 강도다리 정자의 냉장보존을 위해 희석액으로 인공정장(artificial seminal plasma, ASP), Stein's solution (SS), 해산어류용 생리식염수(marine fish Ringer's solution, MFRS)를 이용하여 각각 $0^{\circ}C,\;2^{\circ}C,\;4^{\circ}C$에서 30일간 보존하면서 정자의 활성을 검토하였다. 강도다리 정자의 냉장보존을 위해서 희석액으로는 SS가 가장 효과적이었으며 보존온도는 $0^{\circ}C$에서 정자의 활성이 가장 좋아 30일 동안 높은 운동성을 유지하였다.

Keywords

References

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