Hypoglycemic and Hepatoprotective Effects of Betaine on Streptozotocin-Induced Diabetic Rats

Streptozotocin-유발 당뇨쥐의 베타인 첨가에 의한 항당뇨 및 간보호 효과

  • Jeong, Jae-Jun (Alcoholic Beverage Research Institute, Daesun Distilling Co., Ltd.) ;
  • Kim, Yong-Taek (Alcoholic Beverage Research Institute, Daesun Distilling Co., Ltd.) ;
  • Seo, Won-Seok (Alcoholic Beverage Research Institute, Daesun Distilling Co., Ltd.) ;
  • Yang, Hyun-Ju (Alcoholic Beverage Research Institute, Daesun Distilling Co., Ltd.) ;
  • Lee, Yong-Soo (Alcoholic Beverage Research Institute, Daesun Distilling Co., Ltd.) ;
  • Cha, Jae-Young (Alcoholic Beverage Research Institute, Daesun Distilling Co., Ltd.)
  • 정재준 (대선주조주식회사 연구소) ;
  • 김용택 (대선주조주식회사 연구소) ;
  • 서원석 (대선주조주식회사 연구소) ;
  • 양현주 (대선주조주식회사 연구소) ;
  • 이용수 (대선주조주식회사 연구소) ;
  • 차재영 (대선주조주식회사 연구소)
  • Published : 2006.08.30


This study was to investigate the effect of betaine on the hypoglycemia and hepatoprotection of streptozotocin(STZ)-induced diabetic rats. Male Sprague-Dawley rats weighing around 280 g were randomly assigned to the three experimental groups: a healthy normal group and two groups with STZ-induced diabetes and fed either control diet or betaine diet. Betaine given to the STZ-diabetic rats had significant effect in lowering the serum glucose concentrations compared to the STZ-diabetic rats. The alanine aminotransferase (AST) and aspartate aminotransferase (ALT) activities and triglyceride contents in serum were dramatically higher in the STZ-diabetic rats, but these increases in relation to diabetes also decreased in the STZ-diabetic rats fed betaine. However, the total-cholesterol concentration in the STZ-diabetic rats was even increased by betaine. The morphology of the pancreatic islets in the normal rats showed a typical round form, but most of the islets in the STZ-diabetic rats showed severe morphological alterations by being markedly destroyed. However, the islet morphology of STZ-diabetic rats given betaine mostly maintained a normal rounded appearance. The present study strongly suggests that the administration of betaine showed a moderate hypoglycemic effect by protecting the pancreatic beta-cells by morphological examination from STZ-induced destruction.

Sprague-Dawley 수컷에 streptozotocin (50 mg/kg body weight)을 복강 주사하여 유발시킨 당뇨군에서 베타인(1%, w/w) 첨가에 의한 항당뇨 및 간보호 효과를 검토하였다. Streptozotocin-유발 당뇨군에서 혈당치 및 fructosamine 농도가 현저히 증가하였고, 이러한 증가는 베타인 투여로 감소하였다. 간 기능의 biomarker로 사용되는 alanine aminotransferase (AST) 및 aspartate aminotransferase (ALT) 활성은 당뇨군에서 현저히 증가한 반면 간보호 효과가 있는 베타인 투여 당뇨군에서는 정상군 수준으로 회복됨으로써 당뇨성 간 손상에 효과가 있는 것으로 나타났다. 혈중 중성지질 농도는 당뇨군에서 현저히 증가하였으나, 베타인 투여에 의해 정상군 수준까지 감소하였으나, 총 콜레스테롤 농도는 베타인 첨가에 의해 증가하였다. 췌장 조직의 조직검사에서 췌도 세포의 정상적인 타원형 모양이 정상군에서는 잘 유지되었으나, Streptozotocin-유발 당뇨군에서는 췌도 세포의 파괴가 일어났으며, 베타인 투여에 의해서는 회복된 것으로 나타났다. 이상의 결과로 볼 때 당뇨군에서 베타인 투여에 의한 혈당강하 효과는 췌장의 췌도세포 파괴 억제에 의한 것으로 시사되었다.



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