Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Determination of Silybin of Nutraceutical Herbal Preparations Using HPLC-PDA

  • Hwang, In-Sook (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Han, Eun-Jung (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Bak, Ju-Sung (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Jin-Gon (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Chough, Nam-Joon (Seoul Metropolitan Government Research Institute of Public Health and Environment)
  • Published : 2006.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

Silymarin is an antihepatotoxic substance isolated from the fruits of silybum mariamum. Possibly due to their antioxidant and membrane stabilizing properties, the compounds was shown to protect various organs and cells against a number of insults (Kvasnicka et al., 2003). Among the main silymarin components, [silybin($SB_A,\;SB_B$, isosilybin ($ISB_A,\;ISB_B$) silydianin (SD) and silychristin (SC)], silybin is the major pharmacologically active compound. Korean Pharmaceutical Codex (2nd ed.) describes silybin as the main substance of Cardus Marianus extract as supportive treatment of chronic inflammatory liver disorders. The aim of this work was to analyze silybin from various preparations containing cardus marianus extract, nicotinamide, and riboflavin (CNR). Nine commercial products were tested using reversed-phase HPLC-PDA assay. The limits of detection and quantification were $0.2\;{mu}g/ml$ and $1\;{mu}g/ml$, respectively. Calibration curve showed a good linearity ($r^2$=1.00000) in the range of $1{\sim}500\;{\mu}g/ml$ of silybin standard solutions.

Keywords

References

  1. Anonymous, Korean Pharmaceutical Codex second edition KFDA, Seoul, 373, 1303-1304 (1998)
  2. Anonymous, The Merck Index thirteenth edition Merck & Co., Inc. NJ. pp. 8607 (2001)
  3. Arnone, Merlini, and Zanarotti, Constituents of silybum marianum, Structure isosilybin and Stereochemistry of Silybin. J. Chem. Soc. Chem. Commun. 696-697 (1979)
  4. Brevoort, The U.S. botanical market-An overview. Herbalgram 36, 49-57 (1996)
  5. Carini, Comoglio, Albano, and Poli, Lipid Peroxidation and irreversible damage in the rat hepatocyte model: Protection by the silybin-phospholipid complex IdB 1016., Biochem. Pharmacol. 43, 2111-2115 (1992) https://doi.org/10.1016/0006-2952(92)90168-I
  6. Campodonico, Collado, Ricci, Pappa, Segall, Pizzorno, Dissolution test for silymarin tablets and capsules. Drug Dev Ind Pharm. 27(3), 261-265 (2001) https://doi.org/10.1081/DDC-100000244
  7. David, Lee and Yanze Liu, Molecular structure and stereochemistry of SilybinA, SilybinB, IsosilybinA, and IsosilybinB, Isolated from Silybum marianum (Milk thistle). J. Nat. Prod 66, 1171-1174 (2003) https://doi.org/10.1021/np030163b
  8. Ding, Tian, Zhang, Gu, Chen, Shi, and Sun, Determination of active component in silymarin by RP-LC and LC/MS, Pharm. Biomed Anal. 26(1), 155-161 (2001) https://doi.org/10.1016/S0731-7085(01)00364-8
  9. Flora, Hahn, Rosen, and Benner, Milk Thistle (silybum marianum)_for the therapy of liver disease. Am, J. Gastroenterol. 93(2), 139 (1998) https://doi.org/10.1111/j.1572-0241.1998.00139.x
  10. Fuk-Luen Lam, Holcomb, and Salvatore, Fusari, Liquid Chromatographic Assay of Ascorbic acid, Nicotinamide, Pyridoxine, Thiamine, and Riboflavin in Multivitamin-Mineral Preparations, J. Assoc, Off. Anal. Chem. 67(5), 1007-1011 (1984)
  11. Gyorgy, Antus, and Foldiak, Pluse radiolysis of silybin: one-electron oxidation of the flavonoid at neutral pH. Radiat. Phys. Chem. 39(1), 81-84 (1992)
  12. Hahn Von, Lehmann, Kurten, Uebel, and Vogel, On the pharmacology and toxicology of silymarin, an antihepatotoxic active principle from silybum marianum (L.) Garertn, Arzneimittelforschung. 18(6), 698-704 (1968)
  13. Kvasnicka, Biiba, Sevcik, Voldrich, and Kratka, Analysis of the active components of silymarin. J. Chromatogr A. 990, 239-245 (2003) https://doi.org/10.1016/S0021-9673(02)01971-4
  14. Lecomte, J., Proprietes pharmacologiques generales de la silybine et de la silymarine chez le rat. Arch. intl. Pharmacodyn. 214, 165-176 (1975)
  15. Lotter, and Wagner, The Stereochemistry of silybin, Z. Naturforsch. 38c, 339-341 (1983)
  16. Mira, Silva, and Manso, Scavenging of reactive oxygen species by silibinin dihemisuccinate. Biochem Pharmacol. 48, 753-7596 (1994) https://doi.org/10.1016/0006-2952(94)90053-1
  17. Pietrangelo, Borella, Casalgrandi, Montosi, Ceccarelli, Gallesi, Giovannini, Gasparetto, and Masini, Antioxidant activity of silybin in vivo during long-term iron overload in rats. Gastroenterology. 109, 1941-1949 (1995) https://doi.org/10.1016/0016-5085(95)90762-9
  18. Quaglia, Bossu, Donati, Mazzanti, and Brandt, Determination of silymarine in the extract from the dried silybum marianum fruits by high performance liquid chromatography and capillary electrophoresis. J. Pharm. Biomed. Anal. 19, 435-442 (1999) https://doi.org/10.1016/S0731-7085(98)00231-3
  19. Rickling, Hans, Kramarczyk, Krumbiegel, and Weyhenmeyer, Two high-performance liquid chromatographic assays for the determination of free and total silibinin diastercomers in plasma using column switching with electrochemical detection and reversed-phase chromatography with ultraviolet detection. J. Chromatagr. B. 670, 267-277 (1995) https://doi.org/10.1016/0378-4347(95)00168-9
  20. Takahara, Ohta, and Hirobe, Stimulatory effects of Silibinin on the DNA synthesis in partially hepatectomized rat livers: Non-response in hepatoma and other malignant cell lines. Biachem. Pharmacol. 35, 538-541 (1986) https://doi.org/10.1016/0006-2952(86)90233-9