생약학회지 (Korean Journal of Pharmacognosy)

  • 제37권3호
  • /
  • Pages.143-150
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  • 2006
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  • 0253-3073(pISSN)
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  • 2288-9299(eISSN)
한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

한방방제의 제제학적 연구(1) - 추출방법에 따른 녹용사군자탕의 비교 -

Studies on Pharmaceutical Quality of Oriental Medicinal Preparations (I) - Studies on Decoction of Nokyong-Sagunja-Tang -

  • 최혁재 (경희대학교 동서의학연구소) ;
  • 김은진 (경희대학교 동서의학연구소) ;
  • 김성수 (경희대학교 한의과대학) ;
  • 김남재 (경희대학교 동서의학연구소)
  • Choi, Hyuck-Jae (East-West Medical Research Institute, Kyung Hee University) ;
  • Kim, Eun-Jin (East-West Medical Research Institute, Kyung Hee University) ;
  • Kim, Sung-Soo (College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Nam-Jae (East-West Medical Research Institute, Kyung Hee University)
  • 발행 : 2006.09.30
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초록

Decoction of oriental medicinal preparation is prepared in various manners, and changes of chemical constituents might be occurred depending on the processing techniques. The present study was undertaken to investigate the phγsio-chemical and pharmacological equivalence between two extraction methods of Nokyong-sagunja-Tang. Samples were Prepared as follows ; Sample-I was prepared by simultaneously extracting Sagunja-Tang and velvet antler in one vessel. Sample-lI was prepared by adding velvet antler exact to the water extract of Sagunja-Tang. Both sanples showed similar results of physiochemical parameters such as pH, yield, TLC and HPLC chromatogram, and contents of ginsenoside $Rb_1$ and glycyrrhizin. Also, there were little different between two samples in pharmacological effects such as DPPH free radical scavenging effect, and inhibitory effects on xanthine oxidase, hyaluronidase, trypsin, TBA-Rs formation and hemolysis in vitro. And both samples showed no significant difference in antifatigue activities in mice. These results suggest that there might be little difference between two extraction process when velvet antler added to Sagunia-Tang.

키워드

참고문헌

  1. 이상인 등 공편역 (1992) 방제학, 29, 도서출판 영림사, 서울
  2. 동의학연수소 편저 (1994) 동의학개론, 333, 여강출판사, 서울
  3. 식품의약품안전청 (2005) 대한약전외 한약(생약) 규격집, 99, 식품의약품안전청, 서울
  4. 이상인, 지형준 (1998) 대한약전외 한약(생약) 규격집 주해, 103, 한국메디칼 인덱스사, 서울
  5. Pathak, N. N., Pattanaik, A. K., Patra, R. C. and Arora, B. M. (2001) Mineral composition of antlers of three deer species reared in captivity, Small Ruminant Research, 42: 61-65 https://doi.org/10.1016/S0921-4488(01)00218-8
  6. Barling, P. M., Lai, A. K. W. and Nicholson, L. F. B. (2005) Distribution of EGF and its receptor in growing red deer antler, Cell Biology International, 29: 229-236 https://doi.org/10.1016/j.cellbi.2004.12.004
  7. 전국한의과대학 본초학교실 공편저 (1991) 본초학, 545, 도서출판 영림사, 서울
  8. 허준 (동의학연구소 譯) (1994) 동의보감(내경편), 18, 2614, 여강출판사, 서울
  9. Mthing, J. (1996) High-resolution thin-layer chromatography of gangliosides, J. Chromato. A, 720: 3-25 https://doi.org/10.1016/0021-9673(95)00499-8
  10. Li, L., Zhang, J. L., Sheng, Y. X., Ye, G, Guo, H. Z. and Guo, D. A. (2004) Liquid chromatographic method for determination of four active saponins from Panax notoginseng in rat urine using solid-phase extraction, J. Chromato. B, 808: 177-183 https://doi.org/10.1016/j.jchromb.2004.05.002
  11. Kitagawa, I., Chen, W. Z., Taniyama, T., Harada, E., Hori, K., Kobayashi, M. and Ren. J. (1998) Quantitative determination of constituents in various Licorice roots by means of High Performance Liquid Chromatography, Yakugaku Zasshi, 118: 519-528 https://doi.org/10.1248/yakushi1947.118.11_519
  12. Blois, M. S. (1958) Antioxidant determinations by the use of a stable free radical, Nature, 181: 1199-1200 https://doi.org/10.1038/1811199a0
  13. Yokozawa, T., Dong, E., Wu, L. Z., Oura, H. and Nishioka, I. (1996) Antioxidant activity of Wen-Pi-Tang in vitro, Natural Medicines, 50: 243-246
  14. Elliott, A. J., Scheiber, S. A., Thomas, C. and Pardini, R. S. (1992) Inhibition of glutathione reductase by flavonoids, Biochem. Pharmacol., 44: 1603-1608 https://doi.org/10.1016/0006-2952(92)90478-2
  15. Kim, Y. S., Noh, Y. K., Lee, G. I., Kim, Y. K., Lee, K. S. and Min, K. R. (1995) Inhibitory effects of herbal medicines on hyaluronidase activity, Kor. J. Pharmacogn., 26(3): 265-272
  16. Anson, M. L. (1938) The estimation of pepsin, trypsin, papain and cathepsin with hemoglobin, J. Gen. Physiol., 22: 79-89 https://doi.org/10.1085/jgp.22.1.79
  17. Tsutomu, U., Hiroshi, K. and Zen-ichi, O. (1989) Antiinflammatory effect of extract from Phellodendri cortex, J. Med. Pharma. Society for Wakan-Yaku, 6: 158-164
  18. Dai, F., Miao, Q., Zhou, B., Yang, L. and Liu, Z. L. (2006) Protective effects of flavonols and their glycosides against free radical-induced oxidative hemolysis of red blood cells, Life Sciences, 78: 2488-2493 https://doi.org/10.1016/j.lfs.2005.10.009
  19. Moriura, T., Matsuda, H. and Kubo, M. (1995) Pharmacological study on Agkistrodon blomhoffii BOlE V. Antifatigue effect of the 50% ethanol extract in acute weightloaded forced swimming-treated rats. Biol. Pharm. Bull. 19(1): 62-66
  20. Junge, W., Wilke, B., Halabi, A. and Klein, G. (2004) Determination of reference intervals for serum creatinine, creatinine excretion and creatinine clearance with an enzymatic and a modified Jaffinethod, Clinica Chimica Acta, 344: 137-148 https://doi.org/10.1016/j.cccn.2004.02.007
  21. Wroblewskin, F. and LaDue, J. S. (1955) Lactic dehydrogenase activity in blood, Proc. Soc. Exp. Biol. Med., 90: 210-213
  22. Akahori, A. and Kagawa, K. (1978) Quantities of saikosaponins, baicalin, cinnamic aldehyde and cinnamic acid in decoctions and extracts of Saikokeishi-to. Shoyakugaku Zasshi, 32(1): 24-32
  23. Noguchi, M., Kubo, M., Hayashi, T. and Ono, M. (1978) Studies on the pharmaceutical quality evaluation ofthe crude drug preparations used in oriental medicine 'Kampoo'(I)-Precipitation reaction ofthe components of Cotidis Rhizoma and these of Glycyrrhizae Radix or Rhei Rhizoma in decoction solution. Shoyakugaku Zasshi, 32(2): 104-110
  24. 絲川秀治(1987) Chemical changes of constituents on the process of producing various chinese medicinal decoction. 現代東洋醫學, 8(3): 77-82
  25. Duran-Reynals (1939) A general permeability increasing from manmalian testis on blood capillary J. Biol. Med. 11: 601-12
  26. Satosh, M., Andrew, S. M., and Hirohito, K. (2001) Trypsin induces activation and inflammatory mediator release from human eosinophils through protease-activated receptor-2. J. Immunol. 167: 6615-6622 https://doi.org/10.4049/jimmunol.167.11.6615
  27. 김기홍 (1980) 검사성적의 임상적 활용, 162, 고문사, 서울