Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Studies for Component Analysis and Antioxidant Effect, Antimicrobial Activity in Acanthopanax senticosus HARMS

가시오갈피 (Acanthopanax senticosus) 열매의 성분 분석과 항산화 효과 및 항균활성

  • Kim, Min-Kyeong (Division of Biotechnology, Kangwon National University) ;
  • Jin, Ying-Shan (Division of Biotechnology, Kangwon National University) ;
  • Heo, Seong-Il (Division of Biotechnology, Kangwon National University) ;
  • Shim, Tae-Heum (Gangwon Research Institute of Health and Environment) ;
  • Sa, Jae-Hoon (Gangwon Research Institute of Health and Environment) ;
  • Wang, Myeong-Hyeon (Division of Biotechnology, Kangwon National University)
  • 김민경 (강원대학교 생명공학부) ;
  • 김영선 (강원대학교 생명공학부) ;
  • 허성일 (강원대학교 생명공학부) ;
  • 심태흠 (강원도 보건환경연구원 식의약품분석과) ;
  • 사재훈 (강원도 보건환경연구원 식의약품분석과) ;
  • 왕명현 (강원대학교 생명공학부)
  • Published : 2006.09.30
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Abstract

In the present studγ, we investigated the component analγsis, measurements of antioxidant activities and anti-microbial activities from fruit of Acanthopanax senticosus HARMS in order to detect the biological activities and develop novel functional resources. Different solvents extraction ratios were different according to different solvent extraction of Acanthopanax senticosus HARMS. Composition analysis we highly composed as 72.33%. The minerals of different organs were highly composed of potasium as 5951.3 mg/100g. The monosaccharldes are composed of the arabinose, xylose, mannose, galactose, glucose. Antioxidamt activity was measured in water extracts: $57.3\;{mu}/ml$, ethanol extracts $630.1\;{\mu}g/ml$, methanol extracts: $248.5\;{\mu}g/ml$, 71% ethanol extracts: $198.97\;{\mu}g/ml$, 75% methanol exracts: $96.77\;{\mu}g/ml$, chloroform extracts. $1194.83\;{\mu}g/ml$ at $IC_{50}$ value. The antimicrobial activities were observed in extracts from Acanthopanax senticosus HARMS against am negative bacteria and gram positive bacteria.

Keywords

References

  1. Lee, W. T. (1979) Distribution of Acanthopanax plants in korea. Kor. J. Pharmocol. 10: 103-107
  2. 이창복 (2003) 원색대한식물도감, 812. 향문사, 서울
  3. Tang, W. and Eisenbrand, G. (1992) Chinese drugs of plant origin. 1-12, Springer-Verlag, Berlin, Heidelberg
  4. Whang, W. K., Choi, S. B. and Kim, I. H. (1996) Physiolosical activities of mixed extracts of Acantopancis senticosi Radicis Cortex and Eucommiae Cortex. Kor. J. Pharmacogn 27: 65-74
  5. Han, Y. N., Kwon, Y. K. and Han, B. H. (1981) Comparison on the protective effect of the root of Panax ginseng and the Root bark of Acanthopanax senticosus against lipid peroxidation. Kor. J. Pharmacog. 12: 26-30
  6. Lee, Y. S., Jung, S. H., Lim, S. S., J., J., Lee, S. H. and Shin, K. H. (2001) Effects of the water extract from the stem bark of Acanthopanax senticosus on hyperlipidemia in rats. Kor. J. Pharmacogn. 32: 103-107
  7. Jin, K. A., Lee, W. Y, Sung, J. O., Park, Y. H., Hur, S. D. and Choi, M. S. (2000) The contents of chlorogenic acid and eleutheroside E in Eleutherococcus senticosus (Rupr. et Maxim.) Harms. J. Korean For. Soc. 89: 216-222
  8. Wiseman, H. (1996) Dietary influences on membrane function; important in protection against oxidative damage and disease. Nutrit. Biochem. 7: 2-6 https://doi.org/10.1016/0955-2863(95)00152-2
  9. Shin, D. H. (1997) The study course and movement of natural antioxidants. Kor. Food Sci & Tech. 30: 14-18
  10. Frankel, E. N. (1996) Antioxidants in lipid foods and their on food quality. Food Chem. 57: 51-54 https://doi.org/10.1016/0308-8146(96)00067-2
  11. A. O. A. C (1980) Official methods of analysis, 129-133. 4th ed. Association of official analytical chemist. Washington D. C.
  12. 식품약품안전청 (2002) 301-304, 식품공전 별책
  13. Bligh, E. G and Dyer, W. J. (1959) A rapid methods of total lipid extraction and purification. Can. J Biochem. Physil. 37: 911-917 https://doi.org/10.1139/o59-099
  14. Folch, J., Lee, M. and Sloane Stanley, G. H. (1957) A simple methods for the isolation and purification oftotallipids from animal tissues. J. Biol. Chem. 226: 497-509
  15. Metcalfe, L. D. and Schmitz, A. A. (1961) The rapid preparation of fatty acid esters for gas chromatographic analysis. Anal. Chem. 33: 363-364 https://doi.org/10.1021/ac60171a016
  16. Shim, T. H., Han, K. S., Lee, T. J., Cheong, E. H. and Lee, J.K. (1994) Composition oflipid and amino acid in Semisulcospira gottschei tissues. J. Food Hyg. Saf. 9: 81-87
  17. Blakeney, A. B., Harris, P. J., Henry, R. J. and Stone, B. A. (1983) A simple and rapid preparation of auditor acetates for monosaccharide analysis. Carbohydr. Res. 113: 291-299 https://doi.org/10.1016/0008-6215(83)88244-5
  18. Kumaran, A. and Joel Karunakaran, R. (2006) Antioxidant and free radical scavenging activity of and aqueous extract of Coleus aromaticus. Food Chem. 97: 109-114 https://doi.org/10.1016/j.foodchem.2005.03.032
  19. MacLowry, J. D. and Jaqua, M. J. (1970) Detailed methodology and implementation semiautomated serial dilution microtechnique for antimicrobial susceptibility testing. Appl. Microbiol. 20: 46-53
  20. Jin, L. H., Han, S. S. and Choi, Y. S. (2002) Aantioxidant effects of the extracts of Acanthopanax senticosus. Kor. J. Pharmacogn. 33: 359-363
  21. Yoshida, T., Mori, K., Hatano, T., Okumura, T., Uehar, I., Komagoe, K., Fujita, Y, and Okuda. T. (1989) Studies on inhibition mechanism of autooxidation by tannins and flavonoids. V Radical scavenging effects of tannins and related polyphenols on 1,1-diphenyl-2-picrylhydrazyl radical. Chem. Pharm. Bull. 37: 1919-1923 https://doi.org/10.1248/cpb.37.1919
  22. 안진권, 이위영, 오성진, 박유헌, 허성두, 최명석 (2000) 가시오갈피나무의 eleutheroside E 및 chlorogenic acid 성분함량, 한국임학회지 89: 216-222