Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

Dietary Effect of Silk Protein Sericin or Fibroin on Plasma and Epidermal Amino Acid Concentration of NC/Nga Mice

실크 단백질 Sericin 및 Fibroin의 식이 공급이 아토피 피부염 동물 모델 NC/Nga Mice의 혈장과 표피의 유리 아미노산 함량에 미치는 영향

  • Kim, Hyun-Ae (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Park, Kyung-Ho (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Yeo, Joo-Hong (Department of Agricultural Biology, National Institute of Agricultural Science and Technology) ;
  • Lee, Kwang-Gili (Department of Agricultural Biology, National Institute of Agricultural Science and Technology) ;
  • Jeong, Do-Hyeon (Nutrex Co., Ltd.) ;
  • Kim, Sung-Han (Nutrex Co., Ltd.) ;
  • Cho, Yun-Hi (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
  • 김현애 (경희대학교 동서의학대학원 의학영양학과) ;
  • 박경호 (경희대학교 동서의학대학원 의학영양학과) ;
  • 여주홍 (농업진흥청 농업생물부) ;
  • 이광길 (농업진흥청 농업생물부) ;
  • 정도현 ((주)뉴트렉스) ;
  • 김성한 ((주)뉴트렉스) ;
  • 조윤희 (경희대학교 동서의학대학원 의학영양학과)
  • Published : 2006.09.01
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Abstract

Free amino acids in epidermis function as a major component of Natural Moisturizing Factor (NMF), which maintains the optimal level of water in skin even at the low humidity. In fact, the depletion of free amino acids is reported in the epidermis of atopic dermatitis, the skin condition involving dryness. As an effort searching the dietary source for improving the level of water and free amino acid in epidermis, the dietary effects of silk protein, sericin (S) and fibroin (F) on trans epidermal water loss (TEWL), and plasma and epidermal levels of free amino acids were compared in this study. Thirty of male NC/Nga mice, an animal model of atopic dermatitis, were divided into three groups: group CA as an atopic control with control diet, group S: 1% sericin diet and group F: 1% fibroin diet. Ten of male BALB/c mice were served as group C (control group) with control diet. All mice were fed on diet and water ad libitum for 10weeks. Dry skin condition was established in group CA as TEWL was increased (148.7% of group C). In parallel, epidermal level of glutamate, one of major amino acids functioning as NMF, was dramatically decreased and epidermal levels of methionine and alanine were inversely elevated. Dietary supplementation of sericin (group S) reduced TEWL at the similar level with group C and increased epidermal levels of glutamate as well as serine and glycine, the other major amino acids as NMF. Despite a marked decrease of methionine and alanine, the reduction of TEWL and epidermal levels of glutamate, serine and glycine of group F were less than of group S. Furthermore, in contrast to similar levels of other free amino acids in plasma and epidermis of group S and group C, plasma and epidermal levels of other free amino acids, specifically phenylalanine, isoleucine, cysteine and tyrosine in epidermis of group F, were significantly higher than of group C. Together, our data demonstrate that dietary supplementation of sericin is more effective at improving dry skin condition that paralleled with the normalization of free amino acids in plasma and epidermis of NC/Nga mice.

Keywords

References

  1. Loden M. Role of topical emollients and moisturizers in the treatment of dry skin barrier disorder. Am J Clin Dermatol 4: 771-88, 2003 https://doi.org/10.2165/00128071-200304110-00005
  2. Sator PG, Schmidt JB, Honigsmann H. Comparison of epidermal hydration and skin surface lipids in healthy individuals and in patients with atopic dermatitis. J Am Acad Dermatol 48: 352-358, 2003 https://doi.org/10.1067/mjd.2003.105
  3. Rawlings AV, Harding CR. Moisturization and skin barrier function. Dermatol Ther 17: 43-48, 2004 https://doi.org/10.1111/j.1396-0296.2004.04S1005.x
  4. Potter EL, Purser DB, Cline JH. Effect of various energy sources upon plasma free amino acids in sheep. J Nutr 95: 655-663, 1968 https://doi.org/10.1093/jn/95.4.655
  5. Boomgaardt I, McDonald BE. Comparison of fasting plasma amino acid patterns in the pig, rat and chicken. Can J Physiol Pharmacol 47: 392-395, 1969 https://doi.org/10.1139/y69-069
  6. Tabachnick J, Labadie JH. Studies on the biochemistry of epidermis. J Invest Dermatol 54(1): 24-31, 1970 https://doi.org/10.1111/1523-1747.ep12551492
  7. Steven AC, Steinert PM. Protein composition of cornified cell envelopes of epidermal keratinocytes. J Cell Sci 107: 693-700, 1994
  8. Koyame J, Horii I, Kawasaki K, Nakayama Y, Morikawa M, Mitsui T. Free amino acids of stratum corneum as a biochemical marker to evaluate dry skin. J Soc Cosmet Chem 35: 183-195, 1984
  9. Yamamura T, Tezuka T. The water-holding capacity of the stratum corneum measured by 1H-NMR. J Invest Dermatol 93: 160- 164, 1989 https://doi.org/10.1111/1523-1747.ep12277392
  10. Bikowski J. The use of therapeutic moisturizers in various dermatologic disorders. Cutis 68: 3-11, 2001
  11. Aioi A, Tonogaito H, Hamada H, Ra C, Ogawa H, Maibach H, Matsuda H. Impairment of skin barrier function in NC/Nga Tnd mice as a possible model for atopic dermatitis. Br J Dermatol 144: 12-18, 2000 https://doi.org/10.1046/j.1365-2133.2001.03946.x
  12. Lee YW, Lee KG, Yeo JH. Health method with silk. Joongang publishing Co., 2003
  13. Marstein S, Jellum E, Eldjarn L. The concentration of pyroglutamic acid (2-pyrrolidone-5-carboxylic acid) in normal and psoriatic epidermis, determined on a microgram scale by gas chromatography. Clin Chim Acta 49: 389-395, 1973 https://doi.org/10.1016/0009-8981(73)90237-4
  14. Seong K, Kim JY, Lee JH, Park SK, Cho Y. Arctii Fructus is a prominent dietary source of linoleic acid for revrsing epidermal hyperproliferation of guinea pigs. Korean J Nutrition 36: 819- 827, 2003
  15. Chung S, Kong S, Seong K, Cho Y. $\gamma$-Linoleic acid in borage oil reverses epidermal hyperproliferation in guinea pigs. J Nutr 132: 3090-3097, 2002 https://doi.org/10.1093/jn/131.10.3090
  16. Taniguchi Y, Kohno K, Inoue SI, Koya-Miyata S, Okamoto I, Arai N, Iwaki K, Ikeda M, Kurimoto M. Oral administration of royal jelly inhibits the development of atopic dermatitis-like skin lesions in NC/Nga mice. Int Immunopharmacol pp.313-1324, 2003
  17. Masumoto M, Kotani M, Fujita A, Higa S, Kishimoto T, Suemura M, Tanaka T. Oral administration of persimmon leaf extract ameliorates skin symptoms and transepidermal water loss in atopic dermatitis model, NC/Nga. Br J Dermatol 146: 221-227, 2002 https://doi.org/10.1046/j.1365-2133.2002.04557.x
  18. Lee KG, Yeo JH, Lee YW, Kweon HY, Woo SO, Han SM, Kim JH. Studies on industrial utilization of silk protein. Food Science and Industry 36: 25-37, 2003
  19. Lee KG, Yeo JH, Lee YW, Kweon HY, Kim JH. Bioactive and skin-compatible properties of silk sericin. Korean J Seric Sci 43(2): 109-115, 2001
  20. Engel W. The effect of sericin shampoos. Arztl Kosmetol 17: 91- 110, 1987
  21. Kato, N, Sato S, Yamanaka A, Yamada H, Fuwa N, Nomura M. Silk protein, sericin inhibits lipid peroxidation and tyrosinase activity. Biosci Biotech Biochem 62: 145-147, 1998 https://doi.org/10.1271/bbb.62.145
  22. Suto H, Matsuda H, Mitsuishi K. NC/Nga mice: a mouse model for atopic dermatitis. Int Arch Allergy Immunol 120: 70-75, 1999 https://doi.org/10.1159/000053599
  23. Madyarov S, Lee KG, Yeo JH, Nam J, Lee YW. Improved method for the preparation of silk fibroin hydrolysates. Korean J Seric Sci 41(2): 102-110, 2000
  24. The Korean society of food science and nutrition. Amino acid and protein. In: Handbook of Experiments in Food Science and Nutrition, Part of Nutrition, pp.181-189, Hyoil publishing Co, Seoul, 2000
  25. Moore S, Stein WH. Chromatographic determination of amino acids by the use of automatic recording equipment. In: Colowick Sp, Kaplan No, eds. Methods in Enzymology, Vol 6, pp.819-831, Academic Press, New York, 1963
  26. NC/Nga mice In: 2003 Data of laboratory animals, pp.89-91, Japan SLC, Inc, Kwandong, 2003
  27. BALB/c mice In: 1998 Data of laboratory animals, pp.29-30, Japan SLC, Inc, Kwandong, 1998
  28. Rivera S, Lopez-Soriano J, Azcon-Bieto J, Argile JM. Blood amino acid compartmentation in mice bearing lewis lung carcinoma. Cancer Res 47: 5644-5646, 1987
  29. Stegink LD, Shepherd JA, Brummel MC, Murray LM. Toxicity of protein hydrolysate solutions: correlation of glutamate dose and neuronal necrosis to plasma amino acid levels in young mice. Toxicology 2: 285-299, 1974 https://doi.org/10.1016/0300-483X(74)90020-1
  30. Raitala A, Karjalainen J, Oja SS, Kosunen TU, Hurme M. Indoleamine 2,3-dioxygenase (IDO) activity is lower in atopic than in non-atopic individuals and is enhanced by environmental factors protecting from atopy. Mol Immunol 43: 1054-1056, 2006 https://doi.org/10.1016/j.molimm.2005.06.020
  31. von Bubnoff D, Fimmers R, Bogdanow M, Matz H, Koch S, Bieber T. Asymptomtic atopy is associated with increased indoleamine 2,3-dioxygenase activity and interleukin-10 production during seasonal allergen exposure. Clin Exp Allergy 34: 1056-1063, 2004 https://doi.org/10.1111/j.1365-2222.2004.01984.x
  32. Rawlings AV, Scott IR, Harding CR, Bowser PA. Stratum corneum moisturization at the molecular level. J Invest Dermatol 103(5): 731-741, 1994 https://doi.org/10.1111/1523-1747.ep12398620
  33. Groff JL, Gropper SS. Protein. In: Advanced Nutrition and Human Metabolism 3rd ed. pp.178-204, Wadsworth/Thomson learning, Connecticut, 1999
  34. Zhaorigetu S, Sasaki M, Watanabe H, Kato N. Supplemental silk protein, sericin, suppresses colon tumorigenesis in 1,2-dimethylhydrazine-treated mice by reducing oxidative stress and cell proliferation. Biosci Biotechnol Biochem 65(10): 2181-2186, 2001 https://doi.org/10.1271/bbb.65.2181