Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Thermococcus onnurineus sp. nov., a Hyperthermophilic Archaeon Isolated from a Deep-Sea Hydrothermal Vent Area at the PACMANUS Field

  • Bae, Seung-Seob (Marine Biotechnology Center, Korea Ocean Research & Development Institute) ;
  • Kim, Yun-Jae (Marine Biotechnology Center, Korea Ocean Research & Development Institute) ;
  • Yang, Sung-Hyun (Marine Biotechnology Center, Korea Ocean Research & Development Institute) ;
  • Lim, Jae-Kyu (Marine Biotechnology Center, Korea Ocean Research & Development Institute) ;
  • Jeon, Jeong-Ho (Marine Biotechnology Center, Korea Ocean Research & Development Institute) ;
  • Lee, Hyn-Sook (Marine Biotechnology Center, Korea Ocean Research & Development Institute) ;
  • Kang, Sung-Gyun (Marine Biotechnology Center, Korea Ocean Research & Development Institute) ;
  • Kim, Sang-Jin (Marine Biotechnology Center, Korea Ocean Research & Development Institute) ;
  • Lee, Jung-Hyun (Marine Biotechnology Center, Korea Ocean Research & Development Institute)
  • Published : 2006.11.30
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Abstract

A novel hyperthermophilic, anaerobic, heterotrophic archaeon, designated strain $NA1^T$, was isolated from a deep-sea hydrothermal vent area (depth, 1,650 m) within the Papua New Guinea-Australia-Canada-Manus (PACMANUS) field. Cells of this strain were motile by means of polar flagella, coccoid-shaped with a diameter of approximately $0.5-1.0{\mu}m$, and occurred as single cells. Optimal temperature, pH, and NaCl concentration for growth were $80^{\circ}C$, 8.5, and 3.5%, respectively. The new isolate was an obligate heterotroph that utilized yeast extract, beef extract, tryptone, peptone, casein, and starch as carbon and energy sources. Elemental sulfur was required for growth and was reduced to hydrogen sulfide. The G+C content of the genomic DNA was 52.0 mol%. Phylogenetic analysis of the 16S rRNA gene indicated that strain $NA1^T$ belongs to the genus Thermococcus, and the organism is most closely related to T. gorgonarius, T. peptonophilus, and T. celer; however, no significant homology was observed among species by DNA-DNA hybridization. Strain $NA1^T$ therefore represents a novel species for which the name Thermococcus onnurineus sp. novo is proposed. The type strain is $NA1^T$ (=KCTC 10859, =JCM 13517).

Keywords

References

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