Korean Journal of Materials Research (한국재료학회지)

Materials Research Society of Korea (한국재료학회)
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Influence of Anodic Oxidation Film Formed on Titanium onto Cell Attachment and Proliferation

양극 산화에 의해 티타늄 표면에 형성된 산화 피막이 세포 부착 및 성장에 미치는 영향

  • Noh, Se-Ra (Dept. of Dental Materials and Dental Materials Research Institute, School of Dentistry, Chonnam National University) ;
  • Lee, Yong-Ryeol (Dept. of Dental Materials and Dental Materials Research Institute, School of Dentistry, Chonnam National University) ;
  • Song, Ho-Jun (Dept. of Dental Materials and Dental Materials Research Institute, School of Dentistry, Chonnam National University) ;
  • Park, Yeong-Joon (Dept. of Dental Materials and Dental Materials Research Institute, School of Dentistry, Chonnam National University)
  • 노세라 (전남대학교 치의학전문대학원 치과재료학교실 및 치과재료연구소) ;
  • 이용렬 (전남대학교 치의학전문대학원 치과재료학교실 및 치과재료연구소) ;
  • 송호준 (전남대학교 치의학전문대학원 치과재료학교실 및 치과재료연구소) ;
  • 박영준 (전남대학교 치의학전문대학원 치과재료학교실 및 치과재료연구소)
  • Published : 2006.10.27
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Abstract

This study was purposed to evaluate the influence of anodically oxidized film on titanium (Ti) onto MG-63 osteoblast-like cell attachment and activity. Only scratch lines created by polishing were seen in ASR and ANO-1 groups. About $1.5{\mu}m$-thick homogeneous oxide film which has pores of about $0.5{\mu}m$ diameter were formed in ANO-12. The crystalline structure of the oxide films formed by anodization in phosphoric acid electrolyte was $TiP_2O_7$. The total protein amounts of ANO-1 and ANO-12 groups showed higher values of maximum protein amount than that of AS-R group. At 3 days of incubation, total protein amount showed higher value in ANO-2 when comparing to that of AS-R (p<0.05). Based on the results of ALPase activity test, the degree of MG-63 cell differentiation for initial mineralization matrix formation was similar. For all the test groups after 1 day of incubation, MG-63 cells grew healthily in mono-layer with dendritic extensions. After incubation for 3 days, the specimen surfaces were covered more densely by cells, and numerous micro filaments were extruding to the extracellular matrix.

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References

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