Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Toxicity Evaluation of Organochloride Pesticide, Endosulfan and its Metabolites Using Microalgae

미세조류를 이용한 유기염소계 농약 Endosulfan 및 Endosulfan 분해산물의 독성평가

  • Sohn, Ho-Yong (Dept. of Food and Nutrition, Andong National University) ;
  • Kum, Eun-Joo (Dept. of Food and Nutrition, Andong National University) ;
  • Kim, Jong-Sik (Department of Biological Science, Andong National University) ;
  • Lee, Jung-Bok (Department of Biological Science, Andong National University) ;
  • Kwon, Gi-Seok (The School of Bioresource Sciences. Andong National University)
  • Published : 2006.12.28
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Abstract

A growth inhibition assay using Chlorella sp. AG 10002 based on the OECD 201 standard test procedure was applied to the toxicity testing of endosulfan and its reported metabolites. Comparison of dry cell weight, optical density (OD) at 680 nm, and chlorophyll a concentration indicated that optical density at 680 nm of culture broth is convenient, rapid, and accurate method for cell growth. In this microalgae system, the $IC_{50}$ values of endosulfan, endosulfan sulfate, endosulfan lactone, and endosulfan ether were determined as 9.45, 18.8, 18.2 and 37.5 mg/L, respectively. In a while, endosulfan diol did not show a significant toxicity up to 50 mg/L. Since endosulfan is liable at acidic or alkaline conditions, treatment of endosulfan in pH 3, 4, and 11 for 3 days resulted in reduced toxicity, as expected. These results suggested that the microalgae system is useful to evaluate various toxic chemicals and provide a new notion for bioremediation of endosulfan in aqueous systems.

Keywords

References

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