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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Characterization of Choristoneura fumiferana Genes of the Sixth Subunit of the Origin Recognition Complex: CfORC6

  • Wang, Xaiochun (College of Life Science, Henan Agricultural University) ;
  • Carstens, Eric B. (Department of Microbiology and Immunology, Queen's University) ;
  • Feng, Qili (Great Lakes Forestry Centre, Canadian Forest Service)
  • Received : 2006.05.13
  • Accepted : 2006.10.05
  • Published : 2006.11.30
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Abstract

A new protein was cloned and identified as the sixth subunit of Choristoneura fumiferana origin recognition complex (CfORC6). The newly identified 43 kDa protein CfORC6 is much bigger than DmORC6 (25.7 kDa) and HsORC6 (28.1 kDa), though it's 23.85% identical to DmORC6 and 23.81% identical to HsORC6. Although the molecular weight of CfORC6 is close to ScORc6 (50 kDa), CfORC6 is only 14.03% identical to ScORC6. By alignment, it was found that the N-terminal of CfORC6 has about 30% identities with other ORC6s, but about 100aa of C-terminal of CfORC6 has no identity with other ORC6s. Like ScORC6, CfORC6 has many potential phosphorylation sites, (S/T)PXK. Like DmORC6, CfORC6 has leucine-rich region in the relevant site. Northern Blot showed that CfORC6 mRNA is about 2,000nt. Southern Blot confirmed that there is one copy of CfORC6 gene in spruce budworm genome. Western blot showed that infection of Cf124T cells with CfMNPV didn't affect the expression levels of CfORC6, at least up to 26 hr post infection.

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