KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Optimization of Submerged Cultivation of Hericium erinaceum

Hericium erinaceum 액체배양의 최적화

  • Jung, Jae-Hyun (Department of Food Science and Technology, Chungju National University) ;
  • Lee, Keun-Eok (Department of Biotechnology and Engineerign, Kangwon National University) ;
  • Lee, Shin-Young (Department of Biotechnology and Engineerign, Kangwon National University)
  • Published : 2006.04.28
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Abstract

Recently, it has been known that Hericium erinaceum is a one of the very useful functional materials with great attention in mushroom processing industry. In present study, a liquid culture which was not studied systematically until now, was conducted as a method of cultivation for H. erinaceum, and also examined the characteristics of the liquid culture and conditions of process optimization. A good basal medium was selected through the cultivation of 16 species mushroom media and the optimum condition for medium and cultivation were chosen by response surface method. From these results, the optimum condition of medium for mushroom was 3% glucose, 0.2% yeast extract/peptone(1:1) and 0.1% $KH_2PO_4/MgSO_4$(1:1) and also the optimal culture condition was obtained at inoculum of 13.42%, temperature of $22.3^{\circ}C$ and pH of 5.7. The mycelial dry weight of 9 g/I was obtained under these conditions and this amount was about 1.7 times higher than that which were cultivated in basal medium for 8 days.

H. erinaceum은 식용은 물론 각종 약리효과를 나타내어 현재 산업적으로 매우 주목되고 있는 기능성 소재들 중의 하나이다. 본 연구에서는 H. erinaceum의 기능성 소재화 연구의 일환으로, 지금까지 체계적으로 연구된 바 없는 H. erinaceum의 액체배양을 시도하고. 이 버섯 액체배양의 최적화를 수행하였다. 16종 버섯 배지의 플라스크 배양을 통해 우수 기본 배지를 선발하였고, 이의 반응표면분석에 의해 배지 및 배양조건의 최적화를 수행한 결과, 최적 배지 조성은 glucose 3%, yeast extract/peptone(1:1) 0.2%, $KH_2PO_4/MgSO_4$(1:1) 0.2%이었으며, 최적 배양조건은 $22.28^{\circ}C$, pH 5.67, 접종량 13.42%에서 얻어졌다. 이러한 최적 배지 및 배양조건하에서 얻어진 균사체량은 약 9 g/L 이었으며, 이는 기본배지에서 배양 8일 후 얻어진 최대값인 5.40 g/L에 비하면 약 1.7배 향상된 결과이었다. 하지만 H. erinaceum의 액체배양시 낮은 균체량과 비교적 느린 생육이 관찰되어 보다 높은 생산성의 향상을 위해서는 이에 적합한 새로운 형태의 fermenter의 선정 및 새로운 배양 system의 적용이 필요한 것으로 나타났다.

Keywords

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