Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Novel Pathogenetic Mechanism in a Clinical Isolate of Yersinia enterocolitica KU14

  • Sato Yoshinori (Department of Environmental Infectious Disease, Graduate School of Medical Sciences Kitasato University) ;
  • Kaneko Kenichi (Department of Environmental Infectious Disease, Graduate School of Medical Sciences Kitasato University) ;
  • Sasahara Takeshi (Department of Microbiology, School of Medicine, Kitasato University) ;
  • Inoue Matsuhisa (Department of Environmental Infectious Disease, Graduate School of Medical Sciences Kitasato University, Department of Microbiology, School of Medicine, Kitasato University)
  • Published : 2006.02.01
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Abstract

Yersinia enterocolitica induces a broad range of gastrointestinal syndromes, including acute enteritis. We previously reported that the clinical isolate, Y. enterocolitica KU14, which lacks pYV, was still capable of causing clinical infection. The present study demonstrated that KU14 did not trigger the death of macrophages in vitro, unlike WA-314 (ATCC51871, which harbors the pYV virulence plasmid). However, the intracellular growth of KU14 in the macrophages was greater than that of WA-C (ATCC51872, a non-plasmid harboring the derivative pYV plasmid). Treatment with a cholesterol-binding drug $(\beta-cyclodextrin)$ that affected lipid rafts resulted in a dramatic reduction in the inracellular growth of KU14. These data clearly indicate that the enhanced inracellular growth of KU14 is related to lipid raft-mediated infection.

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References

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