Antimicrobial Effect of Lactic acid and Hydrogen Peroxide and Distribution of Vibrio parahaemolyticus from the Incheon Adjacent Sea

인천연안 Vibrio parahaemolyticus의 분포 및 유산과 과산화수소 처리에 의한 항균효과

  • Jang, Jae-Seon (Dept. of Food & Nutrition, Gachon University of Medicine and Science) ;
  • Cho, Woo-Kyoun (Dept. of Food & Nutrition, Gachon University of Medicine and Science) ;
  • Lee, Hye-Jeong (Dept. of Food & Nutrition, Gachon University of Medicine and Science) ;
  • Lee, Jea-Mann (Health and Environment Research Institute, Incheon) ;
  • Kim, Hye-Young (Health and Environment Research Institute, Incheon) ;
  • Kim, Yong-Hee (Health and Environment Research Institute, Incheon)
  • 장재선 (가천의과학대학교 식품영양학과) ;
  • 조우균 (가천의과학대학교 식품영양학과) ;
  • 이혜정 (가천의과학대학교 식품영양학과) ;
  • 이제만 (인천광역시 보건환경연구원) ;
  • 김혜영 (인천광역시 보건환경연구원) ;
  • 김용희 (인천광역시 보건환경연구원)
  • Published : 2006.12.30

Abstract

This study was carried out to investigate the distribution of Vibrio parahaemolyticus in the Incheon adjacent sea, and antimicrobial effect on growth of Vibrio parahaemolyticus in lactic acid and hydrogen peroxide and combination of lactic acid and hydrogen peroxide. The detected strains were compared geographical, months and sample types. The distribution of Vibrio parahaemolyticus was high at Ganghwa county with 66.1%(336 samples), on 7-9 months with 72.4%(386 samples) and from tireland with 75.0%(90 samples), respectively. The minimun inhibitory concentration (MIC) of lactic acid in Vibrio parahaemolyticus were 1250 ppm at pH 6.5 and 7.0, 625 ppm at pH 6.0. respectively. The minimun inhibitory concentration (MIC) of hydrogen peroxide in Vibrio parahaemolyticus were 25 ppm at pH 6.5 and 7.0, 12.5 ppm at pH 6.0, respectively. MICs of combined treatment of lactic acid and hydrogen peroxide in Vibrio parahaemolyticus were 625 ppm of lactic acid with 12.5 ppm of hydrogen peroxide. The correlations between MICs of lactic acid and hydrogen peroxide in Vibrio parahaemolyticus were obtained through the coefficient of determination($R^2$). $R^2$ value were 1.0000. The antimicrobial effect of lactic acid and hydrogen peroxide in Vibrio parahaemolyticus could be confirmed from the result of this experiment.

Keywords

References

  1. 이한기, 이신호, 유영균, 장재선 : 식품위생학, 대학서림, 2005
  2. A. Chiou, and S. K. Chen : Foodborne illness in Taiwan, Food Aust., 43:70-71, 1990
  3. H. C. Yang, S. S. Hong, K. H. Kim, S. H. Chi and H. J. Chung : Distribution of Vibrio vulnificus in Chonnam coastal area, Kor. J. Appl. Microbiol. Biotechnol., 27: 70-74, 1999
  4. 보건복지부, 보건복지통계연보, 2006
  5. N. Hamad, and E. H. Marth : Behavior of Listeria monocytogenes at 7, 13, 25 and $35^{\circ}C$ in trytose broth acidified with acetic, citric or lactic acid. J. Food Protection, 52:688-693, 1989 https://doi.org/10.4315/0362-028X-52.10.688
  6. L. Dominguez, J. F. Garayazabal, E. R. Ferri, J. A. Vazquez, E. Gomezlucia, C. Ambrosio, and G. Suarez : Viability of Listeria monocytogenes in milk treated with hydrogen peroxide. J. Food Protection, 50:636-640, 1987 https://doi.org/10.4315/0362-028X-50.8.636
  7. L. De Vuyst and E. J. Vandamme : Antimicrobial potential of lactic acid bacteria. In Bacteriocins of Lactic Acid Bacteria. Chapman & Hall Inc., New York, 91-142, 1994
  8. H. E. Rubin, T. Nerad and F. Vaughan : Lactate acid inhibition of Salmonella typhimurium in yogurt. J. Dairy Sci. 65, 197-203, 1982 https://doi.org/10.3168/jds.S0022-0302(82)82177-2
  9. 장재선, 이미연, 이제만, 김용희 : 과산화수소와 유산이 Escherichia coli O157:H7, Salmonella Enteritidis 및 Listeria monocytogenes의 증식 억제에 미치는 영향, 대한위생학회지 19(4), 69-75, 2004
  10. 강동훈, 전상수, 정덕화, 조성환 : 남해안연안에 분포되어 있는 Vibrio parahaemolyticus의 성상 및 Grapefruit Seed Extract 처리에 의한 항균효과, J. food Hyg. Safety 9(3), 141-149, 1994
  11. 김상철, 김두희, 박순우 : Vibrio parahaemolyricus의 환경인자에 대한 저항성, Korean J. of Preventive Medicine, 21:183-194, 1988
  12. C. M. Lin, S. S. Moon, M. P. Doyle and K. H. Mcwatters : Inactivation of E. coli O157:H7, Salmonella enteritica Serotype Enteritidis, Listeria monocytogenes on Lettuce by Hydrogen Peroxide and Lactic acid and Hydrogen Peroxide with Mild Heat, J. of Food Protection, 65(8):1215-1220, 2002 https://doi.org/10.4315/0362-028X-65.8.1215
  13. 장재선, 고종명, 김용희 : Staphylococcus aureus와 Bacillus cereus에 대한 유산과 과산화수소의 증식 억제에 미치는 영향, 한국환경보전학회지, 31(2), 115-119, 2005