Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Antioxidation and Inhibition of Matrix Metalloproteinase in UV-irradiated Human Dermal Fibroblast by Selaginella tamariscina

자외선이 조사된 사람 피부 섬유아세포에서 권백의 항산화와 MMP 발현에 미치는 영향에 대한 연구

  • 심관섭 (한불화장품(주) 기술연구소) ;
  • 김진화 (한불화장품(주) 기술연구소) ;
  • 김진희 (한불화장품(주) 기술연구소) ;
  • 이범천 (한불화장품(주) 기술연구소) ;
  • 표형배 (한불화장품(주) 기술연구소)
  • Published : 2006.06.30
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Abstract

In this study. we evaluated anti-aging activity of medical plants that protect the skin cell damage induced by UV irradiation. We have investigated diverse biological activities of Selaginella tamariscina as an anti-aging ingredient of cosmetics. S. tamariscina was found to show scavenging activities of radicals and reactive oxygen species (ROS) with the $IC_{50}$ values of $65.1{\mu}g/mL$ against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and $40.9 {\mu}g/mL$ against superoxide radicals in the xanthine/xanthine oxidase system, respectively. For testing intracellular ROS scavenging activity, the cultured human dermal fibroblasts were analyzed by increase in dichlorofluorescein (DCF) fluorescence upon exposure to UVB $20 mJ/cm^2$ after treatment of S. tamariscina. UVA-induced MMP-1 protein and mRNA expression in human dermal fibroblasts were reduced in a dose-dependent manner by S. tamariscina. Moreover, S. tamariscina inhibited MMP-2 (gelatinase) activity in UVA-irradiated human dermal fibroblasts assayed by zymography and semi-quantitative RT-PCR. Taken together, these results suggest that S. tamariscina may act as an anti-aging agent by Increasing collagen and preventing the skin cell damage induced by UV irradiation, and imply that S. tamariscina nay be useful as a new ingredient for anti-aging cosmetics.

다양한 약용식물의 피부노화에 미치는 영향을 평가하였으며, 이 중 권백(Selaginella tamariscina)은 동양에서 암환자 치료를 위한 전통약용식물로 알려져 있다. 우리는 피부노화에 대한 화장품 소재로 권백에 대한 다양한 생물학적 평가를 하였다. 권백의 항산화 효과를 알아보기 위해 DPPH radical과 superoxide anion radical 소거효과를 측정하였다. 그 결과 DPPH radical의 $IC_{50}$ 값은 $65.1{\mu}g/mL$이고, xanthine/xanthine oxidase에 의한 superoxide anion radical의 $IC_{50}$ 값은 $40.9 {\mu}g/mL$이었다. 세포내 활성산소 소거평가를 위해 사람 진피 섬유아세포(human dermal fibroblast)를 배양하여 UVB $20 mJ/cm^2$에 의해 증가된 세포내 활성산소(ROS)가 권백을 처리함으로써 활성산소 소거효과가 증가하였다. 사람 진피 섬유아세포에서 UVA에 의해 발현된 MMP-1 단백질과 mRNA가 권백에 의해 농도 의존적으로 감소하였다. 뿐만 아니라, 권백은 zymography와 semi-quantitative RT-PCR을 이용하여 UVA 조사된 사람 진피 섬유아세포에 MMP-2 (gelatinase)의 활성 감소를 확인하였다. 결론적으로 권백은 자외선에 의한 세포손상을 보호하여 항노화 화장품의 새로운 소재로 이용될 것으로 사료된다.

Keywords

References

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