Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Gamma Irradiation on Color Changes and Biological Activities of Ethanol Extract of a Mechanically Pressed Juice of Bokbunja (Rubus coreanus Miq.)

감마선 조사에 의한 복분자 착즙액 에탄올 추출물의 색상 및 생리활성 변화

  • Kim, Hee-Jung (Radiation Fond Science and Biotechnology Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Jo, Cheor-Un (Radiation Fond Science and Biotechnology Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Hyun-Joo (Radiation Fond Science and Biotechnology Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Shin, Dong-Hwa (Dept. of Food Science and Technology, Chonbuk National University) ;
  • Son, Jun-Ho (Dept. of Cosmeceuticals, Daegu Hanny University) ;
  • Byun, Myung-Woo (Radiation Fond Science and Biotechnology Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 김희정 (한국원자력연구소 방사선식품.생명공학연구팀) ;
  • 조철훈 (한국원자력연구소 방사선식품.생명공학연구팀) ;
  • 김현주 (한국원자력연구소 방사선식품.생명공학연구팀) ;
  • 신동화 (전북대학교 식품공학과) ;
  • 손준호 (대구한의대학교 화장품소재공학과) ;
  • 변명우 (한국원자력연구소 방사선식품.생명공학연구팀)
  • Published : 2006.03.01
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Abstract

A mechanically pressed juice of Bokbunja (Rubus coreanus Miq.) extract was prepared using 70% ethanol solution. The extract was subjected to gamma-irradiation treatment (20 kGy) and investigated for its change of color and biological activities. Hunter $L^*$ values of the irradiated Bokbunja extract were increased in comparison with the non-irradiated extracts, and the $a^*\;and\;b^*$values decreased by the irradiation treatment. The content of the total phenolic compounds in the non-irradiated and irradiated extracts were 58.4 and 56.5 mg/g, respectively. The 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging activities of non-irradiated and irradiated extracts at a 250 ppm level were 80% and 79%, respectively. Lipid oxidation was retarded by addition of Bokbunja powder. Nitrite scavenging activity was the highest in the Bokbunja powder at pH 1.2 and the effect was not changed by irradiation. The Bokbunja powder showed antimicrobial activity against Salmonella Typhimurium and Bacillus cereus. However, irradiation of Bokbunja did not affect any physiological functions (p>0.05). A Salmonella mutagenicity assay indicated that the irradiated Bokbunja extract did not show any mutagenicity. Therefore, Bokbunja extract could be used in various applications as a functional material, such as ingredients of food and cosmetic, compositions with functions.

본 연구에서는 복분자 착즙액을 에탄올로 추출하여 감마선 조사(20kGy)한 후 색상 및 생리활성의 변화를 살펴보았다. 복분자 에탄올 추출물의 감마선 조사에 의한 색도변화는 명도($L^*$)의 경우 조사 후에 증가한 반면, 적색도($a^*$) 및 황색도($b^*$)의 경우에는 감소하였다. 복분자 에탄올 추출물의 총 폴리페놀 화합물의 함량은 비조사구와 조사구 각각 58.4 및 56.5 mg/g으로 조사후의 유의적인 차이는 없었다. 전자공여능은 복분자 에탄올 추출물 250 ppm 이상의 모든 시료에서 80%이상의 활성을 나타내었으며 추출물(1%)을 돈육에 적용하여 저장시간에 따른 지질산패도를 TBARS로 측정한 결과 추출물을 첨가 시 지질산패도를 억제하는 것으로 확인하였다. 아질산염 소거능도 pH 1.2, 4.2 및 6.0에서 각각 51.1%, 8.3% 및 7.5%로 나타났으며 전자공여능, 지질산패억제 및 아질산염 소거능 모두 조사에 의한 변화는 관찰되지 않았다. 복분자 에탄올 추출물에 대한 항균활성은 농도에 따른 생육 저해효과를 측정한 결과 10,000 ppm에서 B. Cereus 및 S. Typhimurium의 생육이 억제되었으며 Ames test를 이용한 복귀돌연변이 시험에서는 $10,000{\mu}g/plate$이하일 때 복분자 에탄올 추출물의 돌연변이원성이 나타나지 않는 것으로 확인되었다. 따라서, 복분자 추출물에 감마선 조사기술을 이용한다면 산업적으로 활용도가 높은 천연소재를 제조할 수 있으리라 사료된다.

Keywords

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