여드름병변에서 채취한 Propionibacterium acnes의 분리, 동정 및 생화학적 특성 연구

Biochemical Properities, Isolation & Identification of the Propionibacterium acnes Picked from Acne Lesion

  • 이경숙 (경남정보대학 피부미용과) ;
  • 최정숙 (경북도립 경도대학 뷰티디자인과)
  • Lee, Kyung-Suk (Dept. of Skin Care & Beauty Science, Kyungnam College of Information & Technology) ;
  • Choi, Jeung-Sook (Dept. of Beauty Design, Gyeongdo Provincial College)
  • 투고 : 2006.06.12
  • 발행 : 2006.10.31

초록

피부관리실을 방문한 서른명의 얼굴 여드름 부위로부터 30주의 P. acnes를 분리하였다. 이들 균 주들을 형태학적, 생화학적 특징 등을 조사하여 P. acnes로 동정하였으며, 분리된 균 주들간의 생화학적특징은 유의한 차이를 나타내지 않았다. 생물학적 균 주 분류(biotyping)한 결과 comedone을 나타내는 여드름증상으로부터 23주의 biotype I, 구진(papule) 여드름증상으로부터 1주의 biotype II, 구진과 농포의 여드름증상으로부터 5주의 biotype III, 구진(papule) 여드름증상으로부터 1주의 biotype IV가 각각 분리되었으며, biotype V형의 균 주는 분리되지 않았다.

Thirty P. acnes were isolated from the 30 acne patients who visited some skin care shops from Feburary of 2001 to August of 2001. They were identified by morphological features (gram stain), biochemical properties. Only P. acne strains are isolated among Propionibacteria sp. and biochemical properities of P. acnes were not different from P. acnes ATCC 11827 strain significantly. The results of the biotyping was as follow: twenty three strains of biotype I from comedone, 1 strain of biotype II from pauple, 5 strains of biotype III from pauple and pustule, and 1 strain of biotype IV from pauple were isolated respectively.

키워드

참고문헌

  1. 김낙인 (1991) '직업성여드름의 임상과 치료, 임상약학'. 도서출판 고려의학, 서울, pp.54-57
  2. 김문주 (2000) 여대생들의 여드름관리 행태와 인삼을 활용한 여드름 관리에 대한 연구, 한국미용학회지, 6(1), 149-163
  3. 김주덕 . 권경옥 . 박성순 . 권연두 . 이화순 . 김상진 (1997) '신화장품학'. 도서출판 동화기술, 서울, pp.48-51
  4. 김현주 . 임연순 . 최혜영 . 명기범 (1998) 여드름이 삶의 질에 미치는 영향에 관한 연구. 대한피부과학회지, 36(5), 850-854
  5. 성진하 (1999) 여드름을 유발하는 Propionibacterium acne 배양 상징액의 독성과 생약제 추출물의 효과. 건국대학교 대학원 석사학위논문
  6. 안성구 . 이승헌 . 박윤기 (1993) '흔히 보는 피부질환'. 도서출판 고려의학, 서울, pp.64-71
  7. 전장원 . 김중환 (1982) 단순포진의 임상연구와 인삼사포닌연고의 치료효과. 대한피부과학회지, 20(3), 375-381
  8. 정해윤 . 지선영 (1997) 여드름(acne)에 대한 동서의학적 고찰. 경산대학교 동서의학, 46-47
  9. Bojar R.A., Cunliffe W.J. and Holland K.T. (1995) The short-term treatment of acne vulgaris with benzoyl peroxide: effects on the surface and follicular cutaneous microflora. British Journal of Dermatology, 132, 204-208 https://doi.org/10.1111/j.1365-2133.1995.tb05014.x
  10. Dowell, Y.R.,Jr and Hawkins (1977) T.M Laboratory methods in anaerobic microbiology. CDC laboratory Manual. DREW Pub. No.(CDC) 78-8272. CDC, Atlanta, GA
  11. Fitzpatrick T.B., Johnson R.A., WoffK. Polano M.K. and Sunnod D. (1999) 'Cor Atals and Synopsis of Clinical Dermatology', McGraw-Hil
  12. Fulton J.E. and Black E. (1983) 'Dr Fulton's Step by Step Program for Clearing Acne'. Harper & Row pub., New York
  13. Fulton J.E. and Black E. (1983) 'Clearing Acne'. Harper & Row pub., New York
  14. Harris H.H., Downing D.T. and Stewart M.E. (1983) Sustainable rates of sebum secretion in acne patient and matched normal control subjects. J. Am. Acad. Dermatol., 8, 200-203 https://doi.org/10.1016/S0190-9622(83)70023-X
  15. Higaki S., Kitagawa T., Kegoura M., Morohashi M. and Yamagishi T. (2000) Correlation between Propionibacterium acnes biotype, lipase activity and rash degree in acne patients. J. Dermatol., 27(8), 519-522 https://doi.org/10.1111/j.1346-8138.2000.tb02219.x
  16. Higaki S., Kitagawa T., Kagoura M., Morohashi M. and Yamagishi T. (2000) Relationship between Propionibacterium acnes biotype and Jumi-haidoku-to. J. of Dermatology, 27, 653-638
  17. Kishishita M. (1978) The bacteriological and biological significance of P. acnes -The indigenous bacteria of human skin- Biological and serological study on P. acnes isolated from human skin and distribution of sero and biotype. Acta Dermatologica. 73, 17-23
  18. Kligman A.M., Leyden J.J. and Stewart R. (1977) New uses for benzoylperoxide: a broad-spectrum antimicrobial agent. Int. J. Dermatol., 16, 413-417 https://doi.org/10.1111/j.1365-4362.1977.tb00769.x
  19. Marshall J., Leeming J.P. and Holland K.T. (1987) The cutaneous microflora of nonnal human feet. J. Appl. Bacteriol., 62, 139-146 https://doi.org/10.1111/j.1365-2672.1987.tb02391.x
  20. Mills O.H. and Kligman A.M. (1983) The follicular Biopsy. J. Dermatol., 167, 57-63 https://doi.org/10.1159/000249749
  21. Mugglestone C.J. and Rhodes E.I. (1982) The treatment of acne with an anti-androgen/estrogen combination. Clin. Exp. Dermatol., 7, 593-598 https://doi.org/10.1111/j.1365-2230.1982.tb02482.x
  22. Peck G.L., Olsen T.G. and Yoder E.W. (1979) Prolonged remission of cystic and conglobate acne with 13-cis-retinoic acid. New Engl. J. Med., 300, 329-333 https://doi.org/10.1056/NEJM197902153000701
  23. Pierard-Franchimont C., Pierard G.E., and Saint-Leger D. (1991) Comparison of the kinetics of sebum secretion in young women with and without acne. Dermatol., 183, 120-122 https://doi.org/10.1159/000247650
  24. Puhvel S.M. and Sakamote M.A (1977) Reevaluation of fatty acids as inflammatory agents in acne. J. Invest. Dermatol., 68(2), 93-97 https://doi.org/10.1111/1523-1747.ep12491639
  25. Puhvel S.M. and Amirian D.A. (1996) Bacterial flora of comedones. British Journal of Dermatology, 101, 543-548 https://doi.org/10.1111/j.1365-2133.1979.tb11883.x
  26. Resh W. and Stoughton R.B. (1976) Topical clindamycin in the control of acne vulgaris. Cuitis, 17, 551-554
  27. Robert M.L., James J.L. and Kenneth J.M. (1981) The relationship between bacteria and the abnormal follicular keratinization in acne vulgaris. J. of Invest. Dermatology, 169, 325-330
  28. Russel J.J. (2000) Topical therapy for acne. Am. Fam. Physician, 61(2), 357-366
  29. Strauss J.S. and Kligman A.M. (1960) The pathologic dynamics of acne vulgaris. Arch. Dermatol., 62, 779-790
  30. Ushijima T. (1981) Comparsion of bacteria flora in closed pustules of the acne vulgaris on human facial skin and on normal skin of periphery of the acne. Medicine and biology, 102, 121-124
  31. Webster G.F. (1995) Inflammation in acne vulgaris. J. of Am. Acad. of Dermatology, 247-253
  32. Williams, S.T., Sharp, M.E. and Holt, J.G. (1989) 'Bergey's manual of Systemtic Bacteriology vol. 4'. Williams and Wilkins, Baltmore, pp.1346-1349