Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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Cytotoxicity on Human Cancer Cells and Antitumorigenesis of Chungkookjang, a Fermented Soybean Product, in DMBA-Treated Rats

청국장의 암세포생장억제효과 및 흰쥐에서 DMBA 투여에 의한 유방종양발생 억제효과

  • Kwak Chune-Shil (Aging and Physical Culture Research Institute, Seoul National University) ;
  • Kim Mee-Yeon (Aging and Physical Culture Research Institute, Seoul National University) ;
  • Kim Sung-Ae (Department of Food and Nutrition, Hannam University) ;
  • Lee Mee-Sook (Department of Food and Nutrition, Hannam University)
  • 곽충실 (서울대학교 의학연구원 체력과학노화연구소) ;
  • 김미연 (서울대학교 의학연구원 체력과학노화연구소) ;
  • 김성애 (한남대학교 식품영양학과) ;
  • 이미숙 (한남대학교 식품영양학과)
  • Published : 2006.06.01
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Abstract

It is reported that a fermented soybean food, Doenjang, has srong antimutagenic and cytotoxic effect on cancer cells. This study investigated the effect of Chungkookjang, another traditional popular Korean soybean fermented food, on growth of cancer cells: HL-60, SNU-638 and MCF-7, and also its in vivo antitumorigenic effect in DMBA-induced mammary tumor rat model. For the in vitro study, Chungkookjang and steamed soybeans were extracted with ethanol and sequentially fractioned with 5 kinds of solvents differing in grades of polarity such as hexane, dichloromethane, ethylacetate, butanol and water. Almost all Chungkookjang extracts significantly inhibited the growth of HL-60 (human leukemic cancer cell), SNU-638 (human gastric cancer cell) and MCF-7 (human breast cancer cell) when compared to steamed soybean extracts. Butanol fraction of Chungkookjang extract especially showed a remarkable inhibitory effect in all the three kinds of cancer cells. To induce a mammary gland tumor, DMBA (50 mg/BW) was administered to 50 day-old female rats and followed by Chungkookjang or steamed soybean supplemented diets. Freezedried Chungkookjang powder (20% of diet in wet weight) was added to AIN-93G based diet for the Chungkookjang group of rats. Likewise, steamed soybean powder containing equal protein content to that of Chungkookjang powder was supplemented to soybean group of rats. At 13 weeks later, the mammary tumor incidence, average tumor number and tumor weight a rat were lower in Chungkookjang group compared to the control or soybean group. In conclusion, Chungkookjang showed a strong inhibitory effect on cancer cell growth in vitro, as well as a more preventive effect against chemically induced mammary tumorigenesis in vivo, while steamed soybeans did not. Therefore, these results suggest that Chungkookjang acquire its anticancer activity through the fermentation process.

Keywords

References

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