Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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Effect of Docosahexaenoic Acid (DHA) on the Apoptosis of Human Endothelial ECV304 Cells

어유의 Docosahexaenoic Acid (DHA)가 인체혈관 내피세포(ECV304 Cells)에서의 Apoptosis에 미치는 영향

  • Kim Young-Youn (Division of Nutritional Medicine, Family Health and Welfare Center(FHWC), Sungshin Women's University) ;
  • Kim Hyo-Sook (Division of Nutritional Medicine, Family Health and Welfare Center(FHWC), Sungshin Women's University) ;
  • Kim Mae-Ha (Department of Food and Nutrition, Sungshin Women's University) ;
  • Jang Soo-Jeong (Department of Food and Nutrition, Sungshin Women's University) ;
  • Lee Myoung-Sook (Division of Nutritional Medicine, Family Health and Welfare Center(FHWC), Sungshin Women's University)
  • 김영연 (성신여자대학교 가족건강복지센터 영양의학실) ;
  • 김효숙 (성신여자대학교 가족건강복지센터 영양의학실) ;
  • 김매하 (성신여자대학교 식품영양학과) ;
  • 장수정 (성신여자대학교 식품영양학과) ;
  • 이명숙 (성신여자대학교 가족건강복지센터 영양의학실)
  • Published : 2006.06.01
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Abstract

DHA, one of w-3 fatty acids, modulates cell growth or death though the changes of apoptotic signaling in human endothelial ECV304 cells. We investigated the effects of DHA on the changes of apoptotic signaling in human vascular endothelial ECV304 cells using lipid peroxidation (LPO) metabolites. LPO could be originated by dietary polyunsaturated fatty acids such as linoleic acid(LA), arachidonic acid(AA) and docosahexaenoic acid (DHA). DHA caused cell death of ECV304 cells compared to LA, AA or control as evidenced by changes in cell morphology and MTT assay. LPO levels was significantly elevated by 10 fold in DHA-treated ECV 304 cells and caspase-3 activity was increased by DHA corresponding to increasing incubation times compared to control. One of reasons of the cell death in DHA-treated ECV304 cells could be expected that caspase activity, marker for mitochondrial damages, might be triggered by the increasing LPO levels. Our results strongly indicated that DHA induced LPO production has an important role on apoptotic signaling pathway in ECV304 cells. LPO production in endothelial cells which was metabolized by oxidation of dietary PUFA, might be one of risk factors in the initial progression of atherosclerosis.

Keywords

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