International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Cholinergic and Neurokinergic Agonist-induced $Ca^{2+}$ Responses in Rat von Ebner's Gland Acinar Cells

  • Chung, Ki-Myung (Department of Physiology and Neuroscience, College of Dentistry and Research Institute of Oral Sciences, Kangnung National University) ;
  • Kim, Sang-Hee (Department of Physiology and Neuroscience, College of Dentistry and Research Institute of Oral Sciences, Kangnung National University) ;
  • Cho, Young-Kyung (Department of Physiology and Neuroscience, College of Dentistry and Research Institute of Oral Sciences, Kangnung National University) ;
  • Roper, Stephen D. (Department of Physiology and Biophysics, Medical School, University of Miami) ;
  • Kim, Kyung-Nyun (Department of Physiology and Neuroscience, College of Dentistry and Research Institute of Oral Sciences, Kangnung National University)
  • Published : 2006.09.30
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Abstract

Von Ebner's glands (vEG) are minor salivary glands associated with circumvallate and foliate papilla. The secretions of vEG consist of microenvironment of the taste buds in the circumvallate and foliate papillae, and thus saliva from vEG plays a role in the perception of taste. The $Ca^{2+}$ signaling system in rat vEG acinar cell was examined using the $Ca^{2+}$-sensitive fluorescent indicator Fura-2. Agonist-induced increase in intracellular $Ca^{2+}\;([Ca^{2+}]_i)$ was stimulated by carbachol (CCh) and substance P (SP), but not by norepinephrine (NE), and recovered to control levels by their receptor antagonists dose-dependently. The effects were also observed in $Ca^{2+}$-free medium, suggesting mobilization from intracellular $Ca^{2+}$ store. These results in the vEG acinar cell indicate that 1) $[Ca^{2+}]_i$ is at least regulated by muscarinic and neurokininergic (NK1) receptors; 2) the increases in $[Ca^{2+}])i$ activated by CCh and SP are mainly mediated by discharge of cytosolic calcium pool.

Keywords

References

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