대한치과보철학회지 (The Journal of Korean Academy of Prosthodontics)

  • 제44권4호
  • /
  • Pages.466-476
  • /
  • 2006
  • /
  • 0301-2875(pISSN)
  • /
  • 2005-3789(eISSN)
대한치과보철학회 (The Korean Academy of Prosthodonitics)
권호 표지

의치 연성이장재에 대한 Candida albicans의 부착과 침투연구

IN VITRO STUDY ON THE ADHERENCE AND PENETRATION OF CANDIDA ALBICANS INTO DENTURE SOFT LINING MATERIALS

  • 김민주 (고려대학교 임상치의학대학원 고급보철학과) ;
  • 신상완 (고려대학교 임상치의학대학원 고급보철학과) ;
  • 이정열 (고려대학교 임상치의학대학원 고급보철학과)
  • Kim Min-Ju (Department of Advanced Prosthodontics, Graduate School of Clinical Dentistry, Korea University) ;
  • Shin Sang-Wan (Department of Advanced Prosthodontics, Graduate School of Clinical Dentistry, Korea University) ;
  • Lee Jeong-Yeol (Department of Advanced Prosthodontics, Graduate School of Clinical Dentistry, Korea University)
  • 발행 : 2006.08.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Purpose : Colonization of denture soft lining materials by Candida albicans can result in clinical problem, and deterioration of the materials. This study aimed to compare the retention and penetration of C. albicans into four denture soft lining materials commonly used. Materials and methods : Four denture soft lining materials (Coe-comfort$^{(R)}$, Coe-soft$^{(R)}$, GC soft liner$^{(R)}$, and Tissue conditioner$^{(R)}$) discs were prepared to glass slide and dental stone. Adherence of yeast to surfaces was monitored after one hour incubation of standardized washed cell suspension with test disc surfaces. Adherent cells stained with acridine orange were counted fluorescence microscopy. Penetration of yeast into materials bonded with acrylic resin after 1, 2, 3,4, 5, 6 and 7 days incubation was observed through sections stained using acridine orange and estimated to quantitative analysis using radioisotope. Results : There was statistical significance in cell numbers between smooth and rough surfaces(p<0.05). Higher numbers of cells were observed on rough surfaces. There was statistical significance in adherent cell numbers into smooth and rough surfaces individually(p<0.05). According to the increase of incubation periods, the cells penetrated into denture soft lining materials were shown to increase. The differences among all kinds of soft liner were statistically significant(p<0.05),and the largest number of cells penetrated into soft liners was observed in the Coe-soft$^{(R)}$. Conclusion : Initial adherence and penetration of yeast into denture soft lining materials has been influenced by surface roughness and chemical composition of them. The selection of appropriate materials and their fabrication may promote clinical performance.

키워드

참고문헌

  1. Bulad K, Taylor RL. Verran J, McCord JF. Colonization and penetration of denture soft lining materials by Candida albicans. Dent Mater 2004;20: 167-75 https://doi.org/10.1016/S0109-5641(03)00088-5
  2. Nikawa H, Yamamoto T, Hayashi S, Nikawa Y, Hamada T. Growth and/or acid production of Candida albicans on soft lining materials in vitro. J Oral Rehabi 1994;21:585-94 https://doi.org/10.1111/j.1365-2842.1994.tb01173.x
  3. Dorey JL, Blasberg B, MacEntee MI, Conklin RJ. Oral mucosal disorders in denture wearers. J Prosthet Dent 1985; 53:210-3 https://doi.org/10.1016/0022-3913(85)90111-8
  4. Okita N, Orstavik D, Orstavik J, Ostby K. In vivo and in vitro studies on soft denture materials: microbial adhesion and tests for antibacterial activity. Dent Mater 1991;7: 155-60 https://doi.org/10.1016/0109-5641(91)90035-W
  5. Nikawa H, Yamamoto T, Hamada T, Rahardjo MB, Murata H, Nakanoda S. Antifungal effect of zeolite-incorporated tissue conditioner against Candida albicans growth and/or acid production. J Oral Rehabil 1997; 24: 350-7 https://doi.org/10.1046/j.1365-2842.1997.d01-297.x
  6. Waters MG, Williams DW. Jagger RG, Lewis MA. Adherence of Candida albicans to experimental denture soft lining materials. J Prosthet Dent 1997;77:306-12 https://doi.org/10.1016/S0022-3913(97)70188-4
  7. Gruber RG, Lucatarto EM, Molnar EJ. Fungus growth on tissue conditioners and soft denture liners. Rev Dent Liban 1968; 18:36-43
  8. Williamson RT. Clinical application of a soft denture liner: a case report. Quintessence Int 1995;26:413-8
  9. Wright PS, Young KA, Riggs PD, Parker S, Kalachandra S. Evaluating the effect of soft lining materials on the growth of yeast. J Prosthet Dent 1998;79:404-9 https://doi.org/10.1016/S0022-3913(98)70153-2
  10. Khan Z, Martin J, Collard S. Adhesion characteristics of visible light-cured denture base material bonded to resilient lining materials. J Prosthet Dent 1989; 62: 196-200 https://doi.org/10.1016/0022-3913(89)90313-2
  11. Radford DR, Sweet SP, Challacombe SJ, Walter JD. Adherence of Candida albicans to denture-base materials with different surface finishes. J Dent 1998;26:577-83 https://doi.org/10.1016/S0300-5712(97)00034-1
  12. Yamauchi M, Yamamoto K, Wakabayashi M, Kawano J. In vitro adherence of microorganisms to denture base resin with different surface texture. Dent Mater J 1990;9; 19-24 https://doi.org/10.4012/dmj.9.19
  13. Nikawa H, Hamada T, Yamamoto T, Kumagai H. Effects of salivary or serum pellicles on the Candida albicans growth and biofilm formation on soft lining materials in vitro. J Oral Rehabil 1997;24:594-604 https://doi.org/10.1046/j.1365-2842.1997.00539.x
  14. Nikawa H, Jin C, Hamada T, Makihira S, Kumagai H, Murata H. Interactions between thermal cycled resilient denture lining materials. salivary and serum pellicles and Candida albicans in vitro. Part II. Effects on fungal colonization. J Oral Rehabil 2000;27:124-30 https://doi.org/10.1046/j.1365-2842.2000.00485.x
  15. Nikawa H, Jin C, Makihira S, Egusa H, Hamada T, Kumagai H. Biofilm formation of Candida albicans on the surfaces of deteriorated soft denture lining materials caused by denture cleansers in vitro. J Oral Rehabil 2003; 30: 243-50 https://doi.org/10.1046/j.1365-2842.2003.01024.x
  16. Nikawa H, Hayashi S, Nikawa Y, Hamada T, Samaranayake LP. Interactions between denture lining material. protein pellicles and Candida albicans. Arch Oral Biol 1993;38:631-4 https://doi.org/10.1016/0003-9969(93)90132-6
  17. Frisch J, Levin MP, Bhaskar SN. Clinical study of fungal growth on tissue conditioners. J Am Dent Assoc 1968;76:591-2 https://doi.org/10.14219/jada.archive.1968.0084
  18. Abe Y. Ishii M, Takeuchi M, Ueshige M, Tanaka S, Akagawa Y. Effect of saliva on an antimicrobial tissue conditioner containing silver-zeolite. J Oral Rehabil 2004:31 :568-73 https://doi.org/10.1111/j.1365-2842.2004.01267.x
  19. Burns DR, Burns DA, DiPietro GJ, Gregory RL. Response of processed resilient denture liners to Candida albicens. J Prosthet Dent 1987;57:507-12 https://doi.org/10.1016/0022-3913(87)90026-6
  20. Tang RY. Gonzalez JB, Roberts GD. Polyurethane elastomer as a possible resilient material for denture protheses: a microbiological evaluation. J Dent Res 1975; 54: 1039-45 https://doi.org/10.1177/00220345750540050601
  21. Verran J, Maryan CJ. Retention of Candida albicans on acrylic resin and silicone of different surface topography. J Prosthet Dent 1997;77:535-9 https://doi.org/10.1016/S0022-3913(97)70148-3
  22. Taylor R, Maryan C, Verran J. Retention of oral microorganisms on cobalt-chromium alloy and dental acrylic resin with different surface finishes. J Prosthet Dent 1998;80:592-7 https://doi.org/10.1016/S0022-3913(98)70037-X
  23. Price C, Waters MG, Williams DW, Lewis MA, Stickler D. Surface modification of an experimental silicone rubber aimed at reducing initial candidal adhesion. J Biomed Mater Res 2002; 63: 122-8 https://doi.org/10.1002/jbm.10094
  24. Olan-Rodriguez L, Minah GE, Driscoll CF. Candida albicans colonization of surface-sealed interim soft liners. J Prosthodont 2000;9: 184-8 https://doi.org/10.1111/j.1532-849X.2000.00184.x
  25. Graham BS, Jones DW, Burke J, Thompson JP. In vivo fungal presence and growth on two resilient denture liners. J Prosthet Dent 1991;65: 528-32 https://doi.org/10.1016/0022-3913(91)90294-7
  26. Masella RP, Dolan CT, Laney WR. The prevention of the growth of Candida on silastic 390 soft liner for dentures. J Prosthet Dent 1975;33:250-7 https://doi.org/10.1016/S0022-3913(75)80081-3