Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Antioxidative and Antimicrobial Activities of Pleurotus eryngii Extracts Prepared from Different Aerial Part

부위별 새송이버섯 추출물의 항산화 및 향균효과

  • Kim, Hyun-Jeung (Department of Food and Nutrition, Sungshin Women's University) ;
  • Ahn, Myung-Soo (Department of Food and Nutrition, Sungshin Women's University) ;
  • Kim, Gum-Hee (Department of Food Science and Nutrition, Hoseo University) ;
  • Kang, Myung-Hwa (Department of Food Science and Nutrition, Hoseo University)
  • 김현정 (성신여자대학교 식품영양학과) ;
  • 안명수 (성신여자대학교 식품영양학과) ;
  • 김금희 (호서대학교 식품영양학과) ;
  • 강명화 (호서대학교 식품영양학과)
  • Published : 2006.12.31
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Abstract

Antioxidative and antimicrobial activities were measured for the Pleurotus eryngii (P. eryngii) solvent extracts in order to discover new functional activities. In P. eryngii, the powder moisture was 9.0%, and the carbohydrate, crude protein, crude ash and crude fat contents were 63.06, 20.70, 5.20 and 2.0%, respectively. Among the detected minerals, potassium (K) had the highest levels and manganese (Mn) the lowest. The amount of polyphenol in EtEx (Ethanol Extract) was 387 mg% for the whole body, 158 mg% for the stipe, and 593 mg% for the pileus. Higher levels of polyphenol in the entire body were found in the BuEx (Butanol Extract) (594 mg%) and WaEx (Water extract) (404 mg%) of the P. eryngii powder. BuEx had the highest level in the pileus, and EtEx and BuEx were higher than the other extracts in the stipe. The electron donating ability (EDA) of EtEx of the P. eryngii powder was the highest, at 91.12%, for the whole body, while it was the lowest, at 62.90%, in the stipe. In addition, the EDA of WaEx was 90.39% for the whole body. These EDA values were similar to those for tocopherol (93.93%) and BHT (96.72%), supporting the potential of these extracts to act as antioxidants. A number of the extracts were certified to have antimicrobial activities for small number of microorganisms, especially for gram-negative microorganisms. In other words, BuEx and EAEx in the pileus and WaEx in the stipe were found to inhibit the growth of Pseudomonas aeruginosa (gram negative). Additionally, EtEx and WaEx in all parts were shown to act as antimicrobial agents for Escherichia coli of gram negative.

새송이버섯의 전체, 갓, 대 3부분으로 구분하여 일반성분과 총 폴리페놀함량을 측정하고 분획별 추출물의 수율 측정, 전자공여능, SOD 유사활성, 아질산염 소거능 및 항균활성을 측정하였다. 총 폴리페놀은 에틸아세테이트 추출물을 제외하고는 갓, 전체, 대의 순이었고 용매별로는 전체와 갓에서는 부탄올, 물, 에탄올 추출물의 함량이 높았다. 반면 대에서는 에틸아세테이트와 부탄올 추출물의 함량이 높았다. 전자공여능은 0.02% tocopherol, 0.02% BHT와 비교하여 전체와 갓의 물 추출물과 갓의 에탄올 추출물은 유사한 활성을 나타내었으며 에탄올, 부탄올, 물 추출물에서 활성이 높았다. SOD 유사활성과 아질산염 소거능은 갓의 부탄올, 물, 에탄올 추출물에서 높았다. SOD 유사활성은 대조군인 0.02% tocopherol 및 BHT 보다 낮았다. 전반적으로 대보다는 갓 부위의 추출물에서 항산화능이 높게 나타났다. 항균활성 측정에서 그램 양성균에서는 미비하였지만 그램 음성균 특히 Escherichia coli, Pseudomonas aeruginosa에서 에틸아세테이트와 물 추출물의 높은 항균활성이 나타내어 새송이버섯이 높은 항산화 및 항균효과가 확인되었다. 이를 바탕으로 가공 식품에 사용되는 합성 항산화제, 보존제 같은 첨가제로 새송이버섯을 이용한 천연 첨가제의 개발이 가능할 것으로 사료되며 높은 생리활성을 바탕으로 기능성 식품의 소재로 개발가능성이 시사되었다.

Keywords

References

  1. Ma SJ. Effects of the substances from dried mushroom by several organic solvents on the stability of fat. J. Food Sci. 15: 150-154 (1983)
  2. Chung SY, Kim SH, Kim HS, Kang JS, Cheong HS, Kim GJ and KIM HJ. Effects water soluble extract of Ganoderma lucidum, Kale Juice and sodium dextrothyroxine on hormone and lipid metabolism in hyper-cholesterolemic rats 1. Concentrations of triiodothyronine, thyroxine, blood sugar and lipid composition in serum. J. Korean Soc. Food Sci. Nutr. 19: 381-386 (1990)
  3. Song JH, Lee HS, Hwang JK, Chung TY, Hong SR, Park LM Physiological activities of Phellinus ribis extracts. J. Food Sci. Technol. 35: 690-695 (2003)
  4. Park MH, Oh KY, Lee BW. Anti-cancer activity of lentinus edoeds and Pleurtus astreatus. J. Food Sci. Technol. 30: 702-708 (1998)
  5. Park SS, Lee KD, Min TJ. Study on the screening and development of antibiotics in the mushrooms. Korean J. Mycol. 23: 28-36 (1995)
  6. Wang H, Ng TB. Eryngin, a novel antifungal peptide from fruiting bodies of the edible mushroom Pleurotus erngii. Peptides 25: 1-5 (2004) https://doi.org/10.1016/j.peptides.2003.11.014
  7. Kang TS, Kang MS, Sung JM, Kang AS, Shon HR, Lee SY. Effect of Pleurotus erngiion the blood glucose and cholesterol in diabetic rats. Korean J. Mycol. 29: 86-90 (2001)
  8. Hwang YJ, Nam HK, Chang MJ, Noh GW, Kim SH. Effect of lentinus edodes and Pleurotus erngii extracts on proliferation and apoptosis in human colon cancer cell lines. Food Sci. Nutr. 32: 217-222 (2003) https://doi.org/10.3746/jkfn.2003.32.2.217
  9. Kang TS, Jeong HS, Lee MY, Park HJ, Jho TS, Ji ST, Shin MK. Mycelial growth using the natural product and angiotensin converting enzyme inhibition activity of Pleurotus erngii. Korean J. Mycol.31: 175-180(2003) https://doi.org/10.4489/KJM.2003.31.3.175
  10. Hui YF, Den ES, Chi TH. Antioxidant and free radical scavenging activities of edible mushrooms. J. Food Lipids. 9: 35-46 (2002) https://doi.org/10.1111/j.1745-4522.2002.tb00206.x
  11. Jeong CH, Shim KW. QuaIity characteristics of sponge cakes with addition of Pleurotus erngii mushroom powders. Food Sci. Nutr. 33: 716-722 (2004)
  12. Ahn MS, Won JS, Kim HJ, Han MN. A study on the antioxidative and antimicrobial activities of the Chopi solvents extracts. Korean J. Food Culture 19: 170-176 (2004)
  13. AOAC. Official methods of analysis, 15th ed., Assosiation of official analytical chemists society, Washington, D.C., 994 (1990)
  14. Slavin, S. Emission Spectrochemical Analysis, Wiley interscience, New York. p. 171 (1971)
  15. Jeong DH, Jang HK. Food Analysis Method. Samjoogdang, Seoul. p. 159 (1982)
  16. Choi YH, Kim MJ, Lee HS, Yun BS, Hu C, Kwak SS. Antioxidative compounds in aerial parts of potentilla fragariodes. Korean J. Pharmacogn. 29: 79-85 (1998)
  17. Williams BW, Cuvelier ME, Berset C. Use of free radical method to evaluate antioxidant. Lebensm-Wissu Technol. 28: 25-30 (1995) https://doi.org/10.1016/S0023-6438(95)80008-5
  18. Beaucham C, Fridovich I. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 44: 276-287 (1971) https://doi.org/10.1016/0003-2697(71)90370-8
  19. Kato H. Lee IE. Chuyen NV. Kim SB, Hayase F. Inhibition of nitrosamine formation by nondialyzable melanoidins. Agr. Biol. Chem. Tokyo 51: 1333-1338 (1987) https://doi.org/10.1271/bbb1961.51.1333
  20. Ahn MS, Kim HJ. A study on the antioxidative and antimicrobial activities of the applenmint solvents extracts. Sungshin women's university. J. Living Culture Research 15: 33-51 (2001)
  21. Davison PM, Parish ME. Methods for testing the efficacy of food antimicrobials. Food Technol. 1: 148 (1989)
  22. Judie DD. Antimicrobial agents. Food Technol. 40: 104-110 (1986)
  23. Hong KH, Kim BY, Kim HK. Analysis of nutritional components in Pleurotus ferulea. J. Food Sci. Technol. 36: 563-567 (2004)
  24. Kim JY, Moon KD, Lee SD, Cho SH, Kang HI, Yee ST, Seo Kl. Physicochemical properties of Pleurutus eryngii. J. Food Preserv. 11: 347-351 (2004)
  25. Yim SB, Kim MO, Koo SJ, Dertermination of dietary fiber contents in mushrooms. J. Soc. Food Sci, 7: 69-76 (1991)
  26. Kim HK, Choi YJ, Kim KH. Functional activities of microwave-assisted extracts from Flammulina velutipes. Food Sci. Technol. 34: 1013-1017(2002)
  27. Kim HK, Choi YJ, Jeong SW, Kim KH. Functional activities of microwave-assisted extracts from Lyophyllum ulmarium. Food Preserv. 9: 385-390 (2002)
  28. Song JH, Lee HS, Hwang JK, Han JW, Ro JG, Keum DH, Park KM. Physiological activity of Sarcodon aspratus extracts. Korean J. Food Sci. 23: 172-179 (2003)
  29. Lee GD, Chang HG, Kim HK. Antioxidative and nitrite-scavenging activities of edible Mushrooms. Korean J. Food Sci. 29: 432-436 (1997)