Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Comparison of Expression Pattern of Housekeeping Genes in Mice fed Genetically Modified Rice

유전자 이입에 따른 GM쌀 섭취 마우스의 Housekeeping Gene 발현 패턴 비교

  • Lee, Dong-Yeob (Department of Food Science & Biotechnology, Kyungpook National University) ;
  • Heo, Jin-Chul (Food & Bio-industry Research Institute, Kyungpook National University) ;
  • Lee, Kyu-Hyun (Department of Food Science & Biotechnology, Kyungpook National University) ;
  • Kim, Dong-Ho (Department of Food Science & Biotechnology, Kyungpook National University) ;
  • U, Sang-Uk (Department of Food Science & Biotechnology, Kyungpook National University) ;
  • Cho, Hyun-Suk (National Institute of Agricultural Biotechnology, RDA) ;
  • Lee, Sang-Han (Department of Food Science & Biotechnology, Kyungpook National University)
  • 이동엽 (경북대학교 생명식품공학부) ;
  • 허진철 (경북대학교 식품생물산업연구소) ;
  • 이규현 (경북대학교 생명식품공학부) ;
  • 김동호 (경북대학교 생명식품공학부) ;
  • 우상욱 (경북대학교 생명식품공학부) ;
  • 조현석 (농업생명공학연구원 생물안전성과) ;
  • 이상한 (경북대학교 생명식품공학부)
  • Published : 2007.12.31
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Abstract

To evaluate the human risk of long-term intake of genetically modified (GM) rice, we carried out RT-PCR of housekeeping genes. Housekeeping genes, which show highly uniform expression in living organisms during various stages of development and under different environmental conditions, were normalized by RT-PCR. We assessed the expression of 10 common housekeeping genes (18s rRNA, 25S rRNA, UBC, UBQ5, UBQ10, ACT11, GAPDH, eEF-$1{\alpha}$, ${\beta}$-TUB, GAPDH, ${\beta}$-actin, B2m, G6pd2, Gyk, Gus, Hprt, Cyclophlin A, Tfrc, ${\alpha}$-tubulin and RPL13A) in the liver, stomach, small intestine, large intestine, kidney and spleen of mice fed GM or non-GM rice. We found no significant differences in the expression of housekeeping genes between the two groups of mice.

Keywords

References

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