Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Cytotoxic Effects of Decursin from Angelica gigas Nakai in Human Cancer Cells

당귀로부터 정제한 Decursin의 인체암세포주에 대한 세포독성

  • Park, Kyung-Wuk (Dept. of Food Nutrition, Sunchon National University) ;
  • Choi, Sa-Ra (Dept. of Food Nutrition, Sunchon National University) ;
  • Shon, Mi-Yae (Dept. of Food Nutrition, Gyeongsang National University) ;
  • Jeong, Il-Yun (Radiation Application Research Division, Korea Atomic Energy Research Institute) ;
  • Kang, Kap-Suk (Dept. of Culinary Arts, Busan College of Information Technology) ;
  • Lee, Sung-Tae (Dept. of Biology, Sunchon National University) ;
  • Shim, Ki-Hwan (Division of Applied Life Sciences, Graduate School, Gyeongsang National University) ;
  • Seo, Kwon-Il (Dept. of Food Nutrition, Sunchon National University)
  • 박경욱 (순천대학교 식품영양학과) ;
  • 최사라 (순천대학교 식품영양학과) ;
  • 손미예 (경상대학교 식품영양학과) ;
  • 정일윤 (한국원자력연구소 방사선식품생명공학연구팀) ;
  • 강갑석 (부산정보대학 호텔조리과) ;
  • 이성태 (순천대학교 생물학과) ;
  • 심기환 (경상대학교 대학원 응용생명과학부) ;
  • 서권일 (순천대학교 식품영양학과)
  • Published : 2007.11.30
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Abstract

Anticarcinogenic-active compound was isolated and purified from Angelica gigas Nakai. The compound was identified as decursin ($C_{19}H_{20}O_5$; molecular weight 328) by mass, IR spectrophotometry $^1H-NMR$ and $^{13}C-NMR$. The proliferation decreased in a dose dependant fashion in the MCF-7 cells treated with decursin for 24 hours over the concentration of $20{\mu}g/mL$. The $IC_{50}$ value of the decursin treatment for 24 hours were 31.04, 33.60, 27,24, $20.45{\mu}g/mL$ in the SW480, 293, HepG2 and MCF-7 cells, respectively, The growth inhibitory effect was stronger in the MCF-7 cells compared to other cells including 293 of human normal cells. The chromatin condensation, apoptotic body formation and DNA fragmentation were examined in the cells treated with decursin. These results suggest that decursin from Angelica gigas Nakai inhibited the growth through apoptosis in MCF-7 cells.

당귀로부터 항암활성을 화합물을 silicagel column chromatography를 이용하여 분리 및 정제한 후, $^1H$와 $^{13}C-NMR$ MS, HMQC 및 COSY spectrum(500 MHz $CDCl_3$) 분석을 통하여 분자량 328의 decursin임을 구조 동정하였다. 정제된 decursin을 MCF-7에 24시간 처리한 결과 $20{\mu}g/mL$ 이상에서 농도 의존적으로 암세포주의 성장을 억제하였으며, SW480, HepG2, MCF-7및 293과 같은 인체 암세포주에 대한 decurcin의 $IC_{50}$값은 각각 31.04, 27.24, 20.45 및 $33.60{\mu}g/mL$로 나타나, decursin은 인체 정상세포주인 293세포를 포함한 다른 세포에 비하여 MCF-7에 대하여 가장 높은 성장억제 활성을 보여주었다. MCF-7세포에 decursin을 처리한 결과 대조군에서 균일한 핵의 형태가 관찰된 것에 반해 처리군의 경우는 핵의 응축과 apoptotic body를 보였고, DNA 절편이 관찰되었다. 이 결과는 당귀에서 분리한 decursin은 MCF-7세포에서 apoptosis를 통하여 세포의 성장을 억제하는 것을 암시한다.

Keywords

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