Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Alpha-Tocopherol Contents of Peanut Seeds Depend on Varieties and Processing Methods

  • Published : 2007.12.30
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Abstract

The purpose of this study is to establish an extraction and analysis method for $\alpha$-tocopherol, and to then distinguish among varieties. The $\alpha$-tocopherol contents of 22 varieties of peanut seeds were analyzed by HPLC. Peanut seeds of cv. Palkwang were processed in two ways, by boiling and roasting. The $\alpha$-tocopherol contents of the two types of peanuts were compared with fresh seeds without any processing. $\alpha$-Tocopherol was detected at a retention time of 2.95 minutes. Five standards of $\alpha$-tocopherol covering a range of $20{\sim}100{\mu}g/ml$ were made up in 2% isopropyl alcohol/n-hexane and analyzed in duplicate. The $\alpha$-tocopherol contents differed according to extraction temperature. The contents were $85{\mu}g/g$ or less at $10^{\circ}C\;and\;20^{\circ}C$ and $94{\mu}g/g$ at $30^{\circ}C$, but they were decreased at $40^{\circ}C$ or higher. The $\alpha$-tocopherol contents in 22 peanut varieties were $61.36{\sim}96.80{\mu}g/g$ according to variety. Fresh peanuts contained $106.7{\mu}g/g$ of $\alpha$-tocopherol, while boiled peanuts contained $108.8{\mu}g/g$ of $\alpha$-tocopherol, and roasted peanuts contained $109.2{\mu}g/g$ of $\alpha$-tocopherol.

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References

  1. Andreoli, R., M. Careri, P. Manini, G. Mori and M. Musci. 1997. HPLC analysis of fat-soluble vitamins on standard and narrow bore columns with UV, electrochemical and particle beam MS detection. Chromatographia 44: 606-612
  2. Carpenter, A.P. 1979. Determination of tocopherols in vegetable oils. Journal of America Oil Chemistry Socity 63: 328
  3. Ching, L.S. and S. Mohamed. 2001. Alpha-tocopherol content in 62 edible tropical plants. J. Agric. Food Chem. 49: 3101-3105 https://doi.org/10.1021/jf000891u
  4. Delgado-Zamarreno, M.M., M. Bustamante-Rangel, A. Sanchez-Perez and J. Hernandez-Mendez, 2001. Analysis of vitamin E isomers in seeds and nuts with and without coupled hydrolysis by liquid chromatography and coulometric detection. J. Chromatography A. 935: 77-86 https://doi.org/10.1016/S0021-9673(01)00991-8
  5. Goffman, F.D., L. Velasco and W. Thies. 1999. Quantitative determination of tocopherols in single seeds of rapeseed (Brassica napus L.). Fett/Lipid 101: 142-145 https://doi.org/10.1002/(SICI)1521-4133(199904)101:4<142::AID-LIPI142>3.0.CO;2-J
  6. Kamal-Eldin, A., L. Lars- A ke and L. Appelqvist. 1996. The chemistry and antioxidant properties of tocopherols and tocotrienols. Lipds 31(7): 671-701 https://doi.org/10.1007/BF02522884
  7. Kim, S.O., N.S. Kim, B. S. Noh and D.S. Lee. 2002. Liquid chromatographic determination of $\alpha$-, $\beta$-, $\gamma$-, and $\delta$-tocopherol in sesame oils of different origin. Analytical Science & Technology 15(3): 248-255
  8. Kim, U.K. and K.J. Joo. 1994. EPA, DHA and tocopherols contents in fish oil products and fishes. J. Korean Soc. Food Nutr. 23(1): 68-72
  9. Lee, C.H., Y.J. Jung, D.K. Park, C.W. Kim, Y.B. Han, W.C. Lee and J.B. Kim. 1993. Effects of ascorbate and $\alpha$-Tocopherol administration on liver function in chronically ethanol-treated rats. J. Korean Soc. Food Nutr. 22(2): 132-137
  10. Lee, I.B., K.J. Choi, K.K. Yu and K.W. Chang. 1992. Tocopherols and fatty acids in plant seeds from Korea. J. Korean Agric. Chem. Soc. 35(1): 1-5
  11. Liebler, D.C., J.A. Burr, L. Philips and A.J.L. Ham. 1996. Gas chromatography-mass spectrometry analysis of vitamin E and its oxidation products. Anal. Biochem. 236: 27-34 https://doi.org/10.1006/abio.1996.0127
  12. McBride, H.D. and D.H. Evans. 1973. A rapid voltammetiric method for the determination of tocopherol and antioxidants in oil and fats. Anal. Chem. 45(3): 446-449 https://doi.org/10.1021/ac60325a003
  13. Meijboom, P.W. and G.A. Jongenotter. 1979. A quantitative determination of tocotrienols and tocopherols in palm oil by TLC-GLC. J. Am. Oil Chem. Soc. 56(1): 33-35 https://doi.org/10.1007/BF02671757
  14. Park, M.R, S.H. Baek, Y.K. Cheong, M.H. Lee, K.G. Choi and S.J. Yun. 2001. Tocopherol content and composition in peanut. Korean J. Crop Sci. 46(4): 279-283
  15. Pyka, A. and J. Sliwiok. 2001. Chromatographic separation of tocopherols. J. Chromatography A. 935: 71-76 https://doi.org/10.1016/S0021-9673(01)00944-X
  16. Slover, H.T. and J. Lehamann. 1969. Vitamin E in foods: determination of tocopherols and tocotrienols. J. Am. Oil Chem. Soc. 46(8): 417-420 https://doi.org/10.1007/BF02545626
  17. Strohecker, R. and H.M. Henning. 1966. Vitamin assay-tasted methods. CRC press. Cleveland. pp. 283-308
  18. Thompson, J.N., M. Erodody and W.B. Maxwell. 1972. Chromatographic separation and spectrophotofluorometric determination of tocopherols using hydroxyalkoxypropyl sephadex. Anal. Chem. 50: 267-280 https://doi.org/10.1021/ac50024a004
  19. Waltking, A.E., M. Kiernan and G.W. Bleffert, 1977. Evaluation of rapid polarographic method for determining tocopherols in vegetable oils and oil-based products. JAOAC 60(4): 890-894