Toxicological Research

  • 제23권4호
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  • Pages.347-352
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  • 2007
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  • 1976-8257(pISSN)
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  • 2234-2753(eISSN)
한국독성학회 (Korean Society of Toxicology & Korea Environmental Mutagen Society)
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복분자 추출물이 Lipopolysaccharide로 유도된 간 손상에 대한 항산화 효과

Anti-Oxidative Effects of Rubus coreanum Miquel Extract on Hepatic Injury Induced by Lipopolysaccharide

  • 김인덕 (신라대학교 의생명과학대학 제약공학과) ;
  • 강금석 (신라대학교 의생명과학대학 제약공학과) ;
  • 권륜희 (신라대학교 의생명과학대학 제약공학과) ;
  • 하배진 (신라대학교 의생명과학대학 제약공학과)
  • Kim, In-Deok (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Kang, Kum-Suk (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Kwon, Ryun-Hee (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Ha, Bae-Jin (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
  • 발행 : 2007.12.31
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초록

The protective effects of Rubus coreanum Miquel (RCM) extract against LPS-induced hepatotoxicity were studied in rats. Squrague-Dawley rats were intraperitoneally administered the RCM at 100 mg/kg per day for three weeks. Then single dose of LPS (5 mg/kg) was injected into rats. Four hours later, they were anesthesized with ether and dissected. We examined the levels of glutamate oxaloacetate transaminase (AST), glutamate pyruvate transaminase (ALT), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) in sera, superoxide dismutase (SOD) in mitochondrial fraction and catalase (CAT), glutathione peroxidase (GPx) in liver homogenate. LPS-treatment markedly increased the levels of AST, ALT, ALP, LDH and significantly decreased those of SOD, CAT and GPx. But RCM-pretreatment decreased the levels of AST, ALT, ALP and LDH by 57.9%, 37.4%, 62% and 69% respectively and increased those of SOD, CAT and GPx by 82.9%, 64.2% and 96.7% respectively. Subsequently, the protective effects of RCM was evaluated through histopathological examination of liver tissue. The LPS treatment increased the state of necrosis and cirrhosis surrounding the central veins (CV) and sinusoid, but RCM-treatment decreased the state of necrosis and cirrhosis in the liver tissue. These results demonstrated that protective effects of RCM against LPS-induced hepatotoxicity.

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참고문헌

  1. Aebi, H. (1984). Catalase in vitro methods. Enzymology, 105,121-126 https://doi.org/10.1016/S0076-6879(84)05016-3
  2. Ambrogi, L.P. (1982). Manual of histological and special staining technics. Armed. Forces. Institute of Pathology, 33, 407-445
  3. Beauchamp, C. and Fridovich, I. (1971). Superoxide dismutase improved assays and an assay applicable to acrylamide gels. Anal. Biochem., 44, 276-287 https://doi.org/10.1016/0003-2697(71)90370-8
  4. Borek, C. (2001). Antioxidant health effects of aged garlic extract. The Journal of Nutrition, 131, 1010-1025
  5. Cha, H.S., Park, K.S. and Park, K.M. (2001). Physiological activities of Rubus coreanus Miquel. Korean J. Food. Sci. Technol., 33, 409-415
  6. Dan, B. and Andrew, G. (1993). Chinese herbal medicine; materia medica. Eastland Press, 8, 375-392
  7. Dantzer, R., Bluthe, R.M., Gheusi, G., Cremona, S., Laye, S., Parnet, P. and Kelley, K.W. (1998). Molecular basis of sickness behavior. Annals N. Y. Acad. Sci., 856, 126-134
  8. Enomoto, N., Yamashina, S. and Kono, H. (1999). Development of a new simple rat model of early alcohol-induced liver injury based on sensitization of kupffer cells. Hepatology, 29, 1680-1689 https://doi.org/10.1002/hep.510290633
  9. Fridovich, I. (1986). Biologic effects of the superoxide radicals. Arch. Biochem. Biophysm., 247, 1-15 https://doi.org/10.1016/0003-9861(86)90526-6
  10. Gabriel, L.P. and Hewitt, R.W. (1982). Principles and methods of toxicology. Raven Press, 8, 407-445
  11. Gutteridge, J.M.C., Beard, A.P.C. and Quinlan, G.J. (1983). Superoxide-dependent lipid peroxidation; Problem with the use of catalase as a specific probe for Fenton-derived hydroxyl radicals. Biochem. Biophys. Res. Commun., 117, 901-907 https://doi.org/10.1016/0006-291X(83)91681-9
  12. Halliwell, B. (1990). How to charactrize a biological antioxidant. Free Radic. Res. Commun., 9, 1-12 https://doi.org/10.3109/10715769009148569
  13. Jean, L., Bret, D. and Chistophe, C. (1997). Systemic capsaicin pretreatment fails to block the decrease in food-motivated behavior induced by lipopolysaccharide and interleukin-1$\beta$. Brain Research Bul., 42, 443-449 https://doi.org/10.1016/S0361-9230(96)00370-X
  14. Jeong, C.S., Jung, K.W. and Jeong, J.S. (1999). Hepatoprotective effect of subfractions of Carthamus tinctorius L. Semen on the reversal of biotransformation enzyme activities in $CCl_{4}$-induced hepatotoxic rats. J. Fd. Hyg. Safety, 14, 172-178
  15. Kim, E.J., Lee, Y.J., Shin, H.K. and Park, J.H. (2005). Induction of apoptosis by the aqueous extract of Rubus coreanum in HT-29 human colon cancer cells. Nutrition, 21, 1141-1148 https://doi.org/10.1016/j.nut.2005.02.012
  16. Kim, H.C. and Lee, S.I. (1991). Comparision of the pharmacological effects of kinds of Rubi Fructus. J. Herbology, 6, 3-12
  17. Kim, T.J. (1997). Korean resources plants. Seoul University Republished, 11, 276-285
  18. Kuresh, A.Y. and James, A.J. (2001). A possible emerging role of phytochemicals in improving age-related neurological dysfunctions: A multiplicity of effects. Free Radical Biology. and Med., 30, 583-594 https://doi.org/10.1016/S0891-5849(00)00510-4
  19. Kwak. J.S., Kim, K.S. and Kim, D.H. (1995). The changes of the liver tissue induced by lipopolysaccharide. The Kyungpook University Medical Journal, 36, 507-521
  20. Lawrence, R.A and Burk, R.F. (1976). Glutathione peroxidase activity in selenium-deficient rat liver. Biochem. Biophys. Res. Commun., 71, 952-958 https://doi.org/10.1016/0006-291X(76)90747-6
  21. Lee, H.Y. (2003). Screening of biological activities of the extracts from Rubus coreanus Miq. Korean J.. Medicinal Crop. Sci., 11, 5-12
  22. Lee, M.W. (1995). Phenolic compounds from the leaves of Rubus coreaum. Korean J. Pharmacogn., 39, 200-204
  23. Lee, Y.A. and Lee, M.W. (1995). Tannins from Rubus coreanum. Korean J. Pharmacogn., 6, 27-30
  24. Lowry, O.H., Rosenbrough, N.J., Farr, A.S. and Randall, R.J. (1951): Protein measurenment with folin phenol reagent. J. Biol. Chem., 193, 256-261
  25. Middleton, E. (1996). Biological properties of plant flavonoids an overview. Int. J. Pharmcognosy, 34, 344-348 https://doi.org/10.1076/phbi.34.5.344.13245
  26. Park, J.H., Lee, H.S., Mun, H.C., Kim, D.H., Seong, N.S., Jung, H.G., Bang, J.K. and Lee, H.Y. (2004a). Effect of Ultrasonification process on enhanscement of immunostimulatory activity of Epherdra sinica Stapf and Rubus coreanus Miq. Korean J. Biotechnol. Bioeng., 19, 113-117
  27. Park, J.H., Lee, H.S., Mun, H.C., Kim, D.H., Seong, N.S., Jung, H.G., Bang, J.K. and Lee, H.Y. (2004b). Improvement of anticancer activation of ultrasonificated extracts from Acanthopanax senticosus Harms, Ephedra sinica Stapf and Rubus coreanus Miq. Korean J. Biotechnol. Bioeng., 12, 273-278
  28. Rosen, D.R., Jakobisiak, M., Hartmann, R.P., Ortega, S. and Johnston, D.J. (1993). Mutations with familial amyotrophic lateral sclerosis. Nature, 362, 59-62 https://doi.org/10.1038/362059a0
  29. Tainer, J.A., Getzoff, E.D., Richardson, J.S. and Richardson, D.C. (1983). Structure and mechanism of Cu, Zn, superoxide dismutase. Nature, 306, 274-287 https://doi.org/10.1038/306274a0
  30. Takaharu, N. and Kiyonori, Y. (1999). Low-dose ${\gamma}$-ray irradiation reduces oxidative damage induced by $CCl_{4}$ in mouse liver. Free Radical Biology & Medicine, 27, 1324-1333 https://doi.org/10.1016/S0891-5849(99)00180-X
  31. Yang, H.M., Lim, S.S., Lee, Y.S., Shin, H.K., Oh, Y.S. and Kim, J.K. (2007). Comparison of the Anti-inflammatory effects of the extracts from Rubus coreanus and Rubus occidentalis. Korean J. Food Sci. Technol., 39, 342-347
  32. Yosjikawa, T., Murakami, M., Yoshida, N., Seto, O. and Kondo, M. (1983). Effects of superoxide dimutase and catalase on disseminated intravascular coagulation in rats. Thromb. Haemostas., 50, 869-872