Toxicological Research

  • 제23권4호
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  • Pages.353-362
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  • 2007
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  • 1976-8257(pISSN)
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  • 2234-2753(eISSN)
한국독성학회 (Korean Society of Toxicology & Korea Environmental Mutagen Society)
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Deoxynivalenol에 의한 생체독성 스크리닝 및 중독증 진단지표 확립

Clinical and Toxico-pathological Parameters for Deoxynivalenol Intoxication in B6C3F1 Mice

  • 김은주 (국립수의과학검역원 독성화학과 독성평가연구실) ;
  • 정상희 (국립수의과학검역원 독성화학과 독성평가연구실) ;
  • 구현옥 (국립수의과학검역원 독성화학과 독성평가연구실) ;
  • 강환구 (국립수의과학검역원 독성화학과 독성평가연구실) ;
  • 조준형 (국립수의과학검역원 독성화학과 독성평가연구실)
  • Kim, Eun-Joo (Toxicological Lab, Toxicological & Chemistry Division, National Veterinary Research & Quarantine Service) ;
  • Jeong, Sang-Hee (Toxicological Lab, Toxicological & Chemistry Division, National Veterinary Research & Quarantine Service) ;
  • Ku, Hyun-Ok (Toxicological Lab, Toxicological & Chemistry Division, National Veterinary Research & Quarantine Service) ;
  • Kang, Hwan-Goo (Toxicological Lab, Toxicological & Chemistry Division, National Veterinary Research & Quarantine Service) ;
  • Cho, Joon-Hyoung (Toxicological Lab, Toxicological & Chemistry Division, National Veterinary Research & Quarantine Service)
  • 발행 : 2007.12.31
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초록

Deoxynivalenol (DON) is a common food borne mycotoxin and occurs predominantly in grains such as wheat, barley, oats, etc. DON induces systemic health problems such as loss of appetite, emesis and diarrhea in both human and farm animals. Reliable diagnostic parameters for DON intoxication are needed to prevent deep health impact. In order to establish useful diagnostic parameters, we investigated clinical signs, hematological values, serum biochemical values, gross-, histo- and toxico-pathological findings in B6C3F1 male mice after oral administration of DON (0.83, 2.5 and 7.5 mg/kg) for 8 days. Body weight gain was significantly decreased at the highest dose of DON. Anorexia, ataxia, for crudness and lack of vigor were observed at the highest dose DON group. In hematological values, the numbers of WBC and platelets and hemoglobin content were reduced with decreased neutrophil and monocytes by 7.5 mg/kg DON. Prothrombin time (PT) and activated partial thromboplastin time (aPTT) were prolonged in a dose-dependent manner and the content of fibrinogen was elevated at high dose of DON. Of serum biochemical values, total protein, globulin, BUN, cholesterol and test-osterone were reduced but total bilirubin and albumin/globulin ratio increased. The enzyme activity of alkaline phosphatase was decreased while that of alanine aminotransferase was elevated. Relative organ weights of thymus, seminal vesicle/prostate and testes were dose-dependently reduced but those of liver and left adrenal gland increased with dose dependency. As for pathological findings, atrophy of thymus, seminal vesicle/prostate and testes and submucosal edema and ulceration in stomach and depletion of lymphocytes in thymus cortex were observed. In conclusion, these clinical, hematological, blood biochemical and patholgical parameters obtained in the present studies can be used for diagnosis of DON-mycotoxicosis, especially, low WBC, platelets, protein, BUN and testosterone and delayed prothrombin time can be available as for reliable diagnostic parameters.

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참고문헌

  1. Betina, V. (1989). Structure-activity relationships among mycotoxins. Chem. Biol. Interact., 71, 105-146 https://doi.org/10.1016/0009-2797(89)90030-6
  2. Creppy, E.E. (2002). Update of survey, regulation and toxic effects of mycotoxins in Europe. Toxicol. Lett., 127, 19-28 https://doi.org/10.1016/S0378-4274(01)00479-9
  3. Forsell, J.H., Witt, M.F., Tai, J.H., Jensen, R. and Pestka, J.J. (1986). Effects of 8-week exposure of the B6C3F1 mouse to dietary deoxynivalenol (vomitoxin) and zearalenone. Fd. Chem. Toxic., 24, 213-219 https://doi.org/10.1016/0278-6915(86)90231-0
  4. Forsyth, D.M., Yoshizawa, T., Morooka, N. and Tuite, J. (1977). Emetic and refusal activity of deoxynivalenol to swine. Appl. Envir. Microbiol., 34, 547
  5. Goyarts, T., Grove, N. and Dänicke, S. (2006). Effect of the Fusarium toxin deoxynivalenol wheat given subchronically or as one single dose on the in vivo protein synthesis of peripheral blood lymphocytes and plasma protein in the pig. Fd. Chem. Toxicol., 44, 1953-1965 https://doi.org/10.1016/j.fct.2006.06.017
  6. Kinser, S., Jia, Q., Li, M., Laughter, A., Cornwell, P.D., Christopher Corton, J. and Pestka, J.J. (2004). Gene expression profiling in spleens of deoxynivalenol-exposed mice: immediate early genes as primary targets. J. Toxicol. Environ. Health, 67, 1423-1441 https://doi.org/10.1080/15287390490483827
  7. Kubena, L.F., Huff, W.E., Harvey, R.B., Corrier, D.E., Philips, T.D. and Rottinghause, G.E. (1989). Individual and combined toxicity of deoxynivalenol and T-2 toxin in broiler chicks. Poult. Sci., 68, 622-626 https://doi.org/10.3382/ps.0680622
  8. Meky, F.A., Turner, P.C., Ashcroft, A.E., Miller, J.D, Qiao, Y.L., Roth, M.J. and Wild, C.P. (2003). Development of a urinary biomarker of human exposure to deoxynivalenol. Fd. Chem. Toxicol., 41, 265-273 https://doi.org/10.1016/S0278-6915(02)00228-4
  9. MHLW (Minisrty of Health, Labour and Welfare) (2006). The Nineteenth seminar for visiting food hygiene experts
  10. Osweiler, G.D., Carr, T.F., Sanderson, T.P., Carson, T.L. and Kinker, J.A. (1995). Water deprivation-sodium ion toxicosis in cattle. J. Vet. Diagn. Invest., 7, 583-585 https://doi.org/10.1177/104063879500700437
  11. Osweiler, G.D. (2000). Mycotoxins. Contemporary issue of food anomal health and productivity. Vet. Clin. North Am. Fd. Anim. Pract., 16, 511-530 https://doi.org/10.1016/S0749-0720(15)30084-0
  12. Pestka, J.J. and Smolinski, A.T. (2005). Deoxynivalenol: toxicology and potential effects on humans. J. Toxicol. Environ. Health, 8, 39-69 https://doi.org/10.1080/10937400590889458
  13. Rotter, B.A. (1996). Toxicology of deoxynivalenol (vomitoxin). J. Toxicol. Environ. Health, 48, 1-34
  14. Thomson, W.L. and Wannemacher, R.W. Jr. (1986). Structurefunction relationships of 12,13-epoxytrichothecene mycotoxins in cell culture: comparison to whole animal lethality. Toxicon., 24, 985-994 https://doi.org/10.1016/0041-0101(86)90004-8
  15. WHO (1990). Selected mycotoxins: Ochratoxin, tricothecenes, ergot. Environ. Health, Crit. No. 105
  16. WHO (2001). Biomarkers in risk assessment: Validity and Validation. Environ. Health, Crit. No. 222