Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
권호 표지

Detection of Escherichia coli(K99), Clostridium perfringens and Cryptosporidium parvum in Diarrhetic Feces of Korean Native Calves

한우 송아지의 설사 분변에서 Escherichia coli(K99), Clostridium perfringens와 Cryptosporidium parvum의 검출

  • Published : 2007.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

The prevalence of E. coli(K99), Clostridium perfringens and Cryptosporidium parvum on acute diarrhea in suckling Korean native calves was evaluated in the field by a veterinary practice. In diagnosis, fecal samples were directly collected from calves that had diarrhea between 2 and 98 days of age. 40 samples were analyzed in October, 2006 and December, 2006. Clostridium perfringens and Cryptosporidium parvum were detected in 15(37.5%) and 4(10.0%) of the samples from diarrhetic calves, respectively. However, E. coli(K99) was not detected in the samples from diarrhetic calves. There was no significant difference(p>0.05) between October(5, 25.0%) and December(10, 50.0%) in incidence of detected Clostridium perfringens from diarrhetic calves. On the other hand, significant differences (p<0.05) in the detection rate of Clostridium perfringens were found between the within 1 month age and all other age groups. In the detection of Cryptosporidium parvum, there was no significant difference(p<0.05) between October (2, 10.0%) and December(2, 10.0%) in the incidence of detected Cryptosporidium parvum from diarrhetic calves. These results suggest that causative agents of calf diarrhea occurred frequently with Clostridium perfringens infection than E. coli(K99) and Cryptosporidium parvum.

Keywords

References

  1. 강문일, 한동운, 정용운, 정도영, 이채용, 이정길, 위성환, 조재진. 한우 송아지의 질병발생과 폐사율 조사. 한국가축 위생학회지 2001; 24: 223-241
  2. 김두, 유영수, 유한상, 윤충근. 한우 송아지의 포유기간 중 의 설사발생에 관한 연구. 대한수의학회지 1990; 30: 255-260
  3. 김종만, 윤용덕, 박정문, 김봉환. 송아지 대장균 Pilus Vaccine 개발에 관한 연구: 1. 송아지 설사원인 대장균의 분포 및 Pilus 정제시험. 대한수의학회지 1986; 26: 97-102
  4. 위성환, 이정길, 강영배, 주후돈, 주이석, 박용호, 최상호. 송아지의 실험적 크립토스포리디움. 대한수의학회지 1995; 35: 107-113
  5. 조현주, 박명호, 임영철, 정삼용, 신정균, 정성목, 서강문. 국내 Holstein 젖소와 한우의 질병 발생 양상에 대한 조사 연구-충북지역을 중심으로-.한국임상수의학회지 2003; 20: 185-197
  6. Acres SD, Saunders JR, Radostits OM. Acute undifferentiated neonatal diarrhea of beef calves: the prevalence of enterotoxigenic E. coli, reo-like (rota) virus and other enteropathogens in cow-calf herds. Can Vet J 1977; 18, 274-280
  7. Baker JC. The clinical manifestations of bovine viral diarrhea infection. Vet Clin North Am Food Anim Pract 1995; 11: 425-445 https://doi.org/10.1016/S0749-0720(15)30460-6
  8. Bjorkman C, Svensson C, Christensson B, de Verdier K. Cryptosporidium parvum and Giardia intestinalis in calf diarrhoea in Sweden. Acta Vet Scand 2003; 44: 145-152 https://doi.org/10.1186/1751-0147-44-145
  9. de Leeuw PW, Ellens DJ, Straver PJ, van Balken JA, Moerman A, Baanvinger T. Rotavirus infections in calves in dairy herds. Res Vet Sci 1980; 29, 135-141
  10. De Rycke J, Bernard S, Laporte J, Naciri M, Popoff MR, Rodolakis A. Prevalence of various enteropathogens in the feces of diarrheic and healthy calves. Ann Rech Vet 1986; 17: 159-168
  11. Firehammer BD, Myers LL. Campylobacter fetus subsp jejuni: its possible significance in enteric disease of calves and lambs. Am J Vet Res 1981; 42: 918-922
  12. Harp JA and Goff JP. Strategies for the control of Cryptospordium parvum infection in calves. J Daily Sci 1998; 81: 289-294 https://doi.org/10.3168/jds.S0022-0302(98)75578-X
  13. Haschek B, Klein D, Benetka V, Herrera C, Sommerfeld-Stur I, Vilcek S, Moestl K, Baumgartner W. Detection of bovine torovirus in neonatal calf diarrhoea in Lower Austria and Styria (Austria). J Vet Med B Infect Dis Vet Public Health 2006; 53: 160-165 https://doi.org/10.1111/j.1439-0450.2006.00936.x
  14. Holley DL, Allen SD, Barnett BB. Enzyme-linked immunosorbent assay, using monoclonal antibody, to detect enterotoxic Escherichia coli K99 antigen in feces of dairy calves. Am J Vet Res 1984; 45: 2613-2616
  15. Isaacson RE, Moon HW, Schneider RA. Distribution and virulence of Escherichia coli in the small intestines of calves with and without diarrhea. Am J Vet Res 1978; 39: 1750-1755
  16. Moore DA, Zeman DH. Cryptosporidiosis in neonatal calves: 277 cases (1986-1987). J Am Vet Med Assoc 1991; 198: 1969-1971
  17. Naciri M, Lefay MP, Mancassola R, Poirier P, Chermette R. Role of Cryptosporidium parvum as a pathogen in neonatal diarrhoea complex in suckling and dairy calves in France. Vet Parasitol 1999; 85: 245-257 https://doi.org/10.1016/S0304-4017(99)00111-9
  18. O'Donoghue P. Cryptospordium and cryptospordiosis in man and animals. Int J Parasitol 1995; 25: 139-195 https://doi.org/10.1016/0020-7519(94)E0059-V
  19. O'Handley RM, Cockwill C, McAllister TA, Jelinski M, Morck DW, Olson ME. Duration of naturally acquired giardiosis and cryptosporidiosis in dairy calves and their association with diarrhea. J Am Vet Med Assoc 1999; 214: 391-396
  20. Reynolds DJ, Morgan JH, Chanter N, Jones PW, Bridger JC, Debney TG, Bunch KJ. Microbiology of calf diarrhoea in southern Britain. Vet Rec 1986; 119: 34-39 https://doi.org/10.1136/vr.119.2.34
  21. Roeder BL, Chengappa MM, Nagaraja TG, Avery TB, Kennedy GA. Experimental induction of abdominal tympany, abomasitis, and abomasal ulceration by intraruminal inoculation of Clostridium perfringens type A in neonatal calves. Am J Vet Res 1988; 49: 201-207
  22. Sinks GD, Quigley JD 3rd, Reinemeyer CR. Effects of lasalocid on coccidial infection and growth in young dairy calves. J Am Vet Med Assoc 1992; 200: 1947-1951
  23. Snodgrass DR, Terzolo HR, Sherwood D, Campbell I, Menzies JD, Synge BA. Aetiology of diarrhoea in young calves. Vet Rec 1986; 119: 31-34 https://doi.org/10.1136/vr.119.2.31
  24. Songer JG, Miskimins DW. Clostridial abomasitis in calves: Case report and review of the literature. Anaerobe 2005; 11: 290-294 https://doi.org/10.1016/j.anaerobe.2004.12.004
  25. Timoney JF, Gillespie JH, Scott FW, Barlough JE. The genus Clostridium. In: Hagan and Bruner's microbilogy and infectious diseases of domestic animals, 8th ed. Comstock Publishing Assoc. 1988: 214-240
  26. Trotz-Williams LA, Jarvie BD, Martin SW, Leslie KE, Peregrine AS. Prevalence of Cryptosporidium parvum infection in southwestern Ontario and its association with diarrhea in neonatal dairy calves. Can Vet J 2005; 46: 349-351