Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Inhibitory Effects of Phyto Extract Mixture (PEM381) on Type I Allergic Reaction

식물추출 복합물(PEM381)의 제I형 알레르기 반응 억제 효과

  • Kim, Kyung-Bum (Technology Research Center, Ben's Lab Co., Ltd.) ;
  • Lee, Eu-Gene (Technology Research Center, Ben's Lab Co., Ltd.) ;
  • Chai, Ok-Hee (Dept. of Anatomy, Chonbuk National University Medical School) ;
  • Song, Chang-Ho (Dept. of Anatomy, Chonbuk National University Medical School) ;
  • Jeong, Jong-Moon (Dept. of Life Science, The University of Suwon)
  • 김경범 ((주)벤스랩 중앙연구소) ;
  • 이유진 ((주)벤스랩 중앙연구소) ;
  • 채옥희 (전북대학교 의학전문대학원 해부학교실) ;
  • 송창호 (전북대학교 의학전문대학원 해부학교실) ;
  • 정종문 (수원대학교 생명과학과)
  • Published : 2007.02.28
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Abstract

The purpose of this study is to investigate the anti type I allergic effects and mechanisms of the phyto extract mixture (PEM381) which contains Camellia sinensis (leaf), Psidium guajava (leaf), and Rosa hybrida (flower). PEM381 was tested for its inhibitory effects on arachidonic acid cascade related enzymes (5-lipoxygenase and cyclooxygenase), the mast cell mediated allergic reaction and passive cutaneous anaphylaxis. $IC_{50}$ value of PEM381 against 5-lipoxygenase was $14.11{\pm}0.51ppm$ while that of positive control (nordihy-droguaiaretic acid) was $0.54{\pm}0.08ppm$. PEM381 also exhibited considerable selective inhibition of cyclooxygenase-2. PEM381 could inhibit both degranulation and histamine release in a dose dependent manner from rat peritoneal mast cells activated by compound 48/80. In addition, oral administration of PEM381 showed an inhibitory effect on passive cutaneous anaphylaxis reaction activated by anti-dinitrophenyl IgE antibody in mice. These results suggest that PEM381 may be useful for the prevention and treatment of type Ⅰ allergy related diseases.

본 연구에서는 녹차(Camellia sinensis)잎 추출물, 구아바(Psidium guajava)잎 추출물 그리고 장미(Rosa hybrida)꽃잎 추출물로 이루어진 식물추출 복합물(PEM381)에 대하여 제I형 알레르기 반응 억제 효능 및 생화학적 기전을 밝히기 위하여 COX 억제 활성, LO 억제 활성, compound 48/80에 의한 랫드 복강 비만세포의 탈과립과 히스타민 유리에 미치는 영향 그리고 수동피부아나필락시스 반응 억제효능을 측정하였다. 그 결과 PEM381은 5-LO $IC_{50}$ 값이 $14.11{\pm}0.51ppm$으로 나타났고, COX-1에 비해 COX-2를 선택적으로 억제하는 것으로 나타났다. 또한 PEM381은 compound 48/80에 의한 비만세포의 탈과립과 히스타민 유리를 농도 의존적으로 억제할 뿐만 아니라 수동피부아나필락시스 반응도 억제하였다. 이상의 결과로 PEM381은 알레르기성 비염, 아토피 피부염 및 기관지천식 같은 제I형 알레르기 질환에 대하여 예방 및 치료에 유용하게 사용될 수 있을 것으로 생각된다.

Keywords

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