Comparison of insecticide susceptibility and enzyme activities of biotype B and Q of Bemisia tabaci

담배가루이 Biotype B와 Q의 약제감수성과 효소활성 비교

  • Kim, Eun-Hee (Dept. of Plant Medicine, Coll. of Agri., Chungbuk National University) ;
  • Sung, Jae-Wook (National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Yang, Jeong-Oh (Dept. of Plant Medicine, Coll. of Agri., Chungbuk National University) ;
  • Ahn, Hee-Geun (Dept. of Plant Medicine, Coll. of Agri., Chungbuk National University) ;
  • Yoon, Chang-Mann (Dept. of Plant Medicine, Coll. of Agri., Chungbuk National University) ;
  • Seo, Mi-Ja (Dept. of Plant Medicine, Coll. of Agri., Chungbuk National University) ;
  • Kim, Gil-Hah (Dept. of Plant Medicine, Coll. of Agri., Chungbuk National University)
  • 김은희 (충북대학교 농업생명환경대학 식물의학과) ;
  • 성재욱 (농업과학기술원 농약평가과) ;
  • 양정오 (충북대학교 농업생명환경대학 식물의학과) ;
  • 안희근 (충북대학교 농업생명환경대학 식물의학과) ;
  • 윤창만 (충북대학교 농업생명환경대학 식물의학과) ;
  • 서미자 (충북대학교 농업생명환경대학 식물의학과) ;
  • 김길하 (충북대학교 농업생명환경대학 식물의학과)
  • Published : 2007.12.30


Mitochondrial 16S DNA sequences of Bemisia tabaci which were collected on rose greenhouse of Iwol and Jinchen in Chungbuk and red pepper field of Miryang, Gyeongnam, were analyzed. The mtCOI PCR product of B. tabaci collected on red pepper field of Miryang were digested with EcoT14I (Sty I) into two fragments 555bp and 311bp, while the PCR product of B. tabaci collected on rose greenhouse of Iwol were digested with Sty I into two fragments of 560bp and 306bp. As a result, B. tabaci collected on red pepper reveal biotype Q and those on rose greenhouse was biotype B. These was difference between two biotypes in insecticide susceptibility, and the biotype B was more susceptible than biotype Q. As a result of foliar systemic test, root-uptake systemic test and residual effect, the biotype B was more susceptible. In case of inhibition effect on enzyme activities of fenitrothion (organophosphorous) and fenothiocarb (carbamate), those of biotype Q was higher than those of biotype B. These results indicate that biotype Q was more resistant than biotype B against 12 insecticides.

충북 진천군 이월면 장미재배지와 경남 밀양시 고추밭에서 채집한 담배가루이에 대해서 16S DNA 염기서열을 분석하였다. 각각의 PCR 산물을 EcoT14 I (Sty I)과 Stu I을 처리한 결과 경남 밀양시 고추밭에서 채집한 담배가루이는 Sty I 에서 555pp와 311bp 두 개의 단편이 만들어져 biotype Q임을 확인하였고, 충북진천군 장미재배지에서 채집한 담배가루이는 Stu I에서 560bp와 3060p 두 개의 단편이 만들어서 biotype B임을 확인하였다. 두 biotype에 대한 12종 살충제로 발육단계별 약제감수성, 침투이행성 및 잔효성을 비교하였고, esterase, acetylcholinesterase (AChE), glutathione S-transferase 등의 효소활성에 미치는 저해정도를 검토하였다. 두 biotype간에 약제감수성 차이가 있었고, biotype B가 Q보다 더 감수성이었다. 엽면침투이행성과 근부침투이행성 및 잔효성에서도 biotype B가 Q보다 더 감수성이었다. 유기인계인 fenitrothion과 카바메이트계인 fenothiocarb의 저해제에 대해서 esterase, acetylcholinesterase (AChE), glutathione S-transferase등의 효소활성에 미치는 영향을 검토한 결과, biotype Q가 B보다 활성이 높게 나타났다. 이상의 결과를 종합하여 볼 때 담배가루이 biotype Q가 B보다 약제에 대해서 저항성임을 알 수 있었다.


  1. Ahm, K. S., K. Y. Lee, M. H. Choi, J. W. Kim and G. H. Kim (2001) Effect of temperature and host plant on development and reproduction of the sweetpotato whitefly, Bemisia tabaci (Hopmoptera: Aleyrodidae). Korean J. Appl. Entomol. 43:203-209
  2. Bedford, I. D., R. W. Briddon, J. K. Briddon, R. C. Rosell and P. G. Markham (1994) Geminivirus transmission and biological characterization of Bemisia tabaci (Gennadius) biotypes from different geographic regions. Ann Appl. BioI. 125:311-325
  3. Bellows, T. S., T. M. Perring, R. J. Gill and D. H. Headrick (1994) Description of a species of Bemisia. Ann. Entomol. Soc. Am. 87: 195-206
  4. Brown, J. K., D. R. Frohlich and R. C. Rosell (1995) The sweetpotato/silverleaf whiteflies: biotypes of Bemisia tabaci germ, or a species complex? Ann. Rev. Entomol. 40:511-534
  5. Burban, C., L. D. C. Fishpool, C. Fauquet, D. Fargette and J. C. Thouvenel (1992) Host associated biotypes within West African population of the whitefly Bemisia tabaci (Germ.) (Hom., Aleyrodidae). J. Appl. Entomol. 113:416-423
  6. Byrne, D. N., P. K. van Bretzel, and C. J. Hoffman (1986) Impact of trap design and placement when monitoring for the banded winged whittly and sweetpotato whitefly(H). Environ. Entomol. 15:300-304
  7. Cottage, E. L. and R. V. Gunning (2006) Buprofezin inhibits acetylcholinesterase activity in B-biotype Bemisia tabaci. Journal of molecular neuroscience : MN, 30 (1-2):39-40
  8. Dennehy, T. J. and L. Williams (1997) Management of resistance in Arizona cotton. Pestic. Sci. 51:398-406
  9. Devine, G. J., I. Ishaaya, A. R. Horowitz and I. Denholm (1999) The response of pyriproxyfen-resistant and susceptible Bemisia tabaci (Homoptera: Aleyrodidae) to pyriproxyfen and fenoxycarb alone and in combination with piperonyl butoxide. Pestic. Sci. 55:405-411
  10. EPPO (2004) Diagnostic protocols for regulated pests, Bemisia tabaci. EPPO Bulletin 34:281-288
  11. Ellman, G. L., K. D. Coutey, V. Andres, J. and B. C. Featherstone (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol. 7:88-95
  12. Frohlich, D. R, I. Torres-Jerez, I. D. Bedford, P. G. Markham, and J.K. Brown (1999) A phylogeographical analysis of the Bemisia tabaci species complex based on mitochondrial DNA markers. Mol. Entomol. 8:1683-1691
  13. Guirao, P., F. Beitia and J. L. Cenis (1997) Biotype determination of Spanish populations of Bemisia tabaci (Homoptera: Aleyrodidae). Bull. Entomol. Res. 87:587-593
  14. Habig, W. H, M. J. Pabst and W. B. Jakoby (1974) Glutathione S-transferase: The first enzymatic step in mecapturic acid formation. J. BioI. Chem. 249:7130-7139
  15. Ha, T. K, I. C. Hwang, J. K. Kim, Y. H. Song, G. H. Kim and Y. M. Yu (2003) Toxicities and control effect of three insecticides to greenhouse whitefly, Trialeurodes vaporariorum and sweetpotato whitefly Bemisia tabaci (Homoptera: Aleyrodidae). Korean J. Pesticide Sci. 3:29-36
  16. Horowitz, A. R. Z. Mendelson, M. Cahill and I. Ishaaya (1999) Managing resistance to the insect growth regulator, pyriproxyfen, in Bemisia tabaci. Pestic. Sci. 55:272-232
  17. Horowitz, A. R. Z. Mendelson and I. Ishaaya (1997) Effect of abamectin mixed with mineral oil on the sweetpotato whitefly (Homoptera: Aleyrodidae). J. Econ. Entomol. 90:349-353
  18. Ishaaya. I., and A. R. Horowitz (1995) Pyriproxyfen, a novel insect growth regulator for controlling whiteflies: mechanism and resistance (Homoptera: Aleyrodidae). Pestic. Sci. 43:227-232
  19. Kim, G. H, Y. S. Lee, I. H. Lee and K. S. Ahn (2000) Susceptibility of sweetpotato whitefly, Bemisia tabaci, (Homoptera: Aleyrodidae) to commercially registered insecticides in Korea. Korean J. Pesticide Sci. 4(1):51-58
  20. Larew, H. G, J. J. Knodel-Montz, R. F. Webb, and J. D. Warthen (1985) Liriomyza trifolii (Burgess) (Diptera: Agromyzidae) control on chrysanthermum by neem seed extract applied to soil. J. Econ Entomol. 78:80-84
  21. Lee, M. L., S. B. Ahn, and W. S. Cho (2000) Morphological characteristics of Bemisia tabaci (Germadius) (Homoptera: Aleyrodidae) and discrimination of their Biotypes in Korea by DNA markers. Korean J. Appl. Entomol. 39:5-12
  22. Lee, M. H., S. Y. Kang, S. G. Lee, J. Y. Choi, G. S. Lee, W. Y. Kim, S. W. Lee, S. G. Kim and K. B. Uhm (2005) Occurrence of the B- and Q-biotypes of Bemisia tabaci in Korea. Korean J. Appl. Entomol. 44(3):169-175
  23. Liu, T. X. and P. A. Stansly (1997) Effects of pyriproxyfen on three species of Encarsia formosa (Hymenoptera: Aphelinidae), endoparasitoids of Bemisia argentifolii (Homoptera: Aleyrodidae). J. Econ. Entomol. 43:227-232
  24. Matsui, M. (1995) Efficiency of Encarsia formosa in suppressing population density of Bemisia tabaci on tomatoes in plastic greenhouse. Jpn. J. Appl. Entomol. Zool. 39:25-31
  25. Palumbo, J. C, A. .Horowitz, N. Prabhaker (2001) Insecticidal control and resistance management for Bemisia tabaci. Crop Protection 20:739-765
  26. Perring, T. M. (2001) The Bemisia tabaci species complex. Crop Protection 20:725-737
  27. Prabhaker, N., N. C. Toscano, T.M. Perring, G. Nuessly, K. Kido and R. R. Youngman (1992) Resistance monitoring of the sweetpotato whitefly (Homoptera: Alcyrodidae) in the Imperial Valley of California. J. Econ. Entomol. 85:1063-1068
  28. Prabhaker, N., N. C. Toscano, and T. J. Henneberry (1999) Comparison of neem, urea, and amitraz as oviposition suppressants and larvicides against Bemisia argentifolii (Homoptera: Aleyrodidae). J. Econ. Entomol. 92(1):40-46
  29. Rauch. N. and R. Nauen (2004) Characterization and molecular cloning of a glutathione S-tranferase from the whitefly Bemisia tabaci (Homoptera: Aleyrodidae). Insect Biochem. Mol. Biol. 34:321-329
  30. Rubinstein, G., S. Morin and H. Cosnek (1999) Transmission of tomato yellow leaf curl geminivirus to imidacloprid treated tomato plants by the whitefly, Bemisia tabaci (Hemoptera : Aleyrodidae). J. Econ. Entomol. 92:658-662
  31. SAS Institute, 2003. SAS/STAT User's Guide: Statistics, version 9.1. SAS Institute, Cary, N.C., U.S.A
  32. Susan, A. C. J. K. Brown and L. H. Donald (2003) Biochemical characterization of biotype-specific esterases in the whitefly, Bemisia tabaci Genn (Homoptera: Aleyrodidae). Insect Biochem. Mol. Biol. 24(7):723-728
  33. Ueda, S. (2006) Simple and Rapid Detection by mtCOI PCR-RFLP to distinguish the Q biotype of Bemisia tabaci. Kyushu Plant Prot. Res. 52:44-48
  34. Ueda, S. and J. Brown (2006) First report of the Q biotype of Bemisia tabaci in Japan by mitochondrial cytochrome oxidase I sequence analysis. Phytoparasitica 34:405-411
  35. Van Asperen, K. J. (1962) A study of housefly esterase by means of sensitive colorimetric method. J. Insect Pathol. 8:401-416
  36. Van Lenteren, J. C. and J. Woets (1988) Biological and integrated pest control in greenhouses. Ann. Rev. Entomol. 33:239-269