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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Induction of Growth Hormone Release by Glycyrrhizae Radix on Rat

  • Lee, Ho-Young (Department of Drug Research & Development, Korea Institute of Oriental Medicine) ;
  • Jung, Dae-Young (Department of Drug Research & Development, Korea Institute of Oriental Medicine) ;
  • Ha, Hye-Kyung (Department of Drug Research & Development, Korea Institute of Oriental Medicine) ;
  • Kang, Sam-Sik (Natural Products Research Institute, Seoul National University) ;
  • Kim, Ju-Sun (Natural Products Research Institute, Seoul National University) ;
  • Kim, Chung-Sook (Department of Drug Research & Development, Korea Institute of Oriental Medicine)
  • Published : 2007.11.30
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Abstract

Induction of growth hormone (GH) by Glycyrrhizae Radix (GR), one of the most popular herbal medicine, and its major ingredients were studied in rat pituitary cells in vitro and in vivo assay. The MeOH extract and the n-hexane (HX) fraction of GR induced rat GH (rGH) release up to 1.89 times ($0.34{\pm}0.04 nM$) and 4.59 times ($0.83{\pm}0.03 nM$), compared to the basal level (p < 0.05). Among many ingredients isolated and purified from GR both glycyrrhetinic acid and glycyrrhizin induced significantly rGH release compared to the control (p < 0.05). After an intravenous injection of rat growth hormone releasing hormone (rGHRH) ($10{\mu}g$/kg) as positive control, in SD rats, $T_{max}$ of plasma rGH level was 10 min, $C_{max}$ was $3.84{\pm}0.01 nM$ (n = 3), and enhanced plasma rGH level returned to the baseline in 90 min. Both $AUC_{0-90}$ (area under the curve) of plasma rGH level after HX fraction and that after rGHRH administration were increased significantly from the basal level, respectively (p < 0.01). In conclusions, HX fraction is the most active fraction of MeOH extract of GR in rGH induction.

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References

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