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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Differentially Expressed Genes under Cold Acclimation in Physcomitrella patens

  • Sun, Ming-Ming (College of Life Science, Capital Normal University) ;
  • Li, Lin-Hui (Department of Biology, China West Normal University) ;
  • Xie, Hua (Beijing AgroBiotechnology Research Center) ;
  • Ma, Rong-Cai (Beijing AgroBiotechnology Research Center) ;
  • He, Yi-Kun (College of Life Science, Capital Normal University)
  • Published : 2007.11.30
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Abstract

Cold acclimation improves freezing tolerance in plants. In higher plants, many advances have been made toward identifying the signaling and regulatory pathways that direct the low-temperature stress response; however, similar insights have not yet been gained for simple nonvascular plants, such as bryophytes. To elucidate the pathways that regulate cold acclimation in bryophytes, we used two PCR-based differential screening techniques, cDNA amplified fragment length polymorphism (cDNA-AFLP) and suppression subtractive hybridization (SSH), to isolate 510 ESTs that are differentially expressed during cold acclimation in Physcomitrella patens. We used realtime RT-PCR to further analyze expression of 29 of these transcripts during cold acclimation. Our results show that cold acclimation in the bryophyte Physcomitrella patens is not only largely similar to higher plants but also displays distinct differences, suggests significant alteration during the evolution of land plants.

Keywords

References

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