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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Systematic Studies of 12S Seed Storage Protein Accumulation and Degradation Patterns during Arabidopsis Seed Maturation and Early Seedling Germination Stages

  • Li, Qing (The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University) ;
  • Wang, Bai-Chen (The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University) ;
  • Xu, Yu (The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University) ;
  • Zhu, Yu-Xian (The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University)
  • Published : 2007.05.31
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Abstract

Seed storage proteins (SSPs) are important for seed germination and early seedling growth. We studied the accumulation and degradation profiles of four major Arabidopsis 12S SSPs using a 2-DE scheme combined with mass spectrometric methods. On the 2-DE map of 23 dpa (days post anthesis) siliques, 48 protein spots were identified as putative full-length or partial $\alpha$, $\delta$ subunits. Only 9 of them were found in 12 dpa siliques with none in younger than 8 dpa siliques, indicating that the accumulation of 12S SSPs started after the completion of cell elongation processes both in siliques and in developing seeds. The length and strength of transcription activity for each gene determined the final contents of respective SSP. At the beginning of imbibition, 68 SSP spots were identified while only 2 spots were found at the end of the 4 d germination period, with $\alpha$, subunits degraded more rapidly than the $\alpha$ subunits. The CRC $\delta$ subunit was found to degrade from its C-terminus with conserved sequence motifs. Our data provide an important basis for understanding the nutritional value of developing plant seeds and may serve as a useful platform for other species.

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References

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