KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Preparation and Characterization of Poly[3-hydroxybutyrate-co-4-hydroxybutyrate] Microsphere

Poly[3-hydroxybutyrate-co-4-hydroxybutyrate] 미립구의 제조 및 특성화

  • Kang, Hye-Su (Department of Chemical Engineering, College of Engineering, Chungbuk National University) ;
  • Kim, Beom-Soo (Department of Chemical Engineering, College of Engineering, Chungbuk National University)
  • 강혜수 (충북대학교 공과대학 화학공학부) ;
  • 김범수 (충북대학교 공과대학 화학공학부)
  • Published : 2007.06.30
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Abstract

Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] microspheres were prepared using solvent evaporation technique. P(3HB-co-4HB) with 3.9 mol% 4HB was synthesized by fed-batch culture of Ralstonia eutropha. The effects of concentration and type of surfactant (Tween 80, sodium dodecylsulfate, and polyvinyl alcohol), addition of dispersion stabilizer (Acacia), concentration of polymer and model drug (bovine serum albumin) on particle size of the microspheres and their in vitro drug release characteristics were investigated. The average particle size of the microspheres decreased with the addition of dispersion stabilizer and increased with the concentration of surfactant, drug and polymer. Amount of drug release increased with decreasing particle size of the microspheres.

Poly (3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] 미립구를 용매증발법으로 제조하였다. 3.9 mol% 4HB 조성의 P(3HB-co-4HB)를 Ralstonia eutropha의 유가식 배양으로부터 합성하였다. 계면활성제의 농도 및 종류(Tween 80, sodium dodecylsulfate, polyvinyl alcohol), 분산안정제 (Acacia)의 첨가, 고분자 및 모델 약물 (bovine serum albumin)의 농도 등이 미립구 입자 크기에 미치는 효과 및 in vitro 약물 방출 특성을 조사하였다. 평균 입자크기는 분산 안정제 첨가시 감소하였으며, 계면활성제, 약물 및 고분자의 농도가 증가할수록 증가하였다. 약물 방출량은 입자 크기가 감소할수록 증가하였다.

Keywords

References

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