Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidant Effects of Red Ginseng Powder on Liver of Benzo(α)Pyrene-Treated Mice

벤조피렌을 투여한 생쥐의 간 조직에서의 홍삼분말의 항산화 효과

  • Kim, Hyun-Jeong (The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Lee, Ji-Won (Dept. of Food and Technology, Keimyung University) ;
  • Ji, Young-Ju (Dept. of Food and Technology, Keimyung University) ;
  • Yu, Me-Hei (Dept. of Food and Technology, Keimyung University) ;
  • Park, Jung-Hyun (The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Lee, Ki-Dong (Daegu Bio Industry Center, Daegu New Technology Agency) ;
  • Lee, In-Seon (The Center for Traditional Microorganism Resources, Keimyung University)
  • 김현정 (계명대학교 전통미생물자원개발 및 산업화연구센터) ;
  • 이지원 (계명대학교 식품가공학과) ;
  • 지영주 (계명대학교 식품가공학과) ;
  • 유미희 (계명대학교 식품가공학과) ;
  • 박정현 (계명대학교 전통미생물자원개발 및 산업화연구센터) ;
  • 이기동 (대구신기술사업단 바이오산업지원센터) ;
  • 이인선 (계명대학교 전통미생물자원개발 및 산업화연구센터)
  • Published : 2007.05.30
Download PDF
⟨ Previous Next ⟩

Abstract

The effects of red ginseng powder on hepatotoxicity in $benzo(\alpha)pyrene\;[B(\alpha)P]$-treated mice were investigated. Male ICR mice were pretreated with red ginseng powder (50 or 100 mg/kg/day, for 5 days, intraperitoneally) before treatment with $B(\alpha)P$ (0.5 mg/kg, i.p, single dose). The ability of red ginseng powder to protect against oxidative damage to the mouse liver was examined by determining the level of lipid peroxide, glutathione, and the antioxidant enzyme activities. The glutathione content depleted by $B(\alpha)P$ were significantly increased by red ginseng powder, but elevation of lipid peroxide content induced by $B(\alpha)P$ was decreased by red ginseng powder. The increased activities of superoxide dismutase, catalase and glutathione peroxidase after $B(\alpha)P$-treatment were decreased by the treatment of red ginseng Powder; however, glutathione S-transferase activity depleted by $B(\alpha)P$ were significantly increased. These results suggest that red ginseng powder can protect against $B(\alpha)P$ intoxification through its antioxidant properties.

홍삼분말이 $B(\alpha)P$의 투여에 의한 간 독성이 유발된 생쥐에서의 글루타치온 및 과산화지질 함량, 항산화효소 활성에 미치는 영향을 살펴보았다. $B(\alpha)P$ 투여에 의해 증가된 혈청내 ALT와 AST의 활성이 홍삼분말 전 처리군에서는 감소하였다. 간 조직중의 글루타치온 함량은 $B(\alpha)P$ 단독군에서는 감소되었다가 홍삼분말 투여시 유의적인 증가를 보였고, 지질과산화물 함량은 $B(\alpha)P$ 투여시 증가되었다가 홍삼분말의 투여시 유의적으로 감소되었다. 간 조직중의 SOD, catalase 그리고 GSH-Px의 활성도 $B(\alpha)P$ 투여로 유의적으로 증가되었다가, 홍삼분말의 투여로 이들 활성이 유의적으로 감소하였다. 반면, GST 활성은 $B(\alpha)P$ 단독군에서는 감소되었다가 홍삼분말 투여시 유의적인 증가를 보였다. 이상의 결과로 홍삼분말은 항산화효소의 활성에 의한 $B(\alpha)P$에 의한 간 손상에 대한 보호효과를 가지는 것으로 사료된다.

Keywords

References

  1. Aktay G, Deliorman D, Ergun E, Ergun F, Yesilada E, Cevik C. 2000. Hepatoprotective effects of turkish folk remedies on experimental liver injury. J Ethernopharm 73: 121-129 https://doi.org/10.1016/S0378-8741(00)00286-5
  2. Yun HS, Chang IM, Chi HJ, Lee SY. 1980. Plants with liver protective activities (IV). Korean J Pharmacog 11: 57-60
  3. Muriel P, Garciapina T, Perez-alvarez V, Mourelle M. 1992. Silymarin protects against paracetamol-induced lipid-peroxidation and liver damage. J Appl Toxicol 12: 439-442 https://doi.org/10.1002/jat.2550120613
  4. Lee BC, Park JO, Ryu BH. 1997. Antioxidative effects of silymarin and silybin purified from Silybum marianum on lipid peroxidation. Korean J Food & Nutr 10: 37-43
  5. Kim BS, Kim HK, Choi JW, Lee CK. 1996. The effects of meliae toosendan fructus on liver function. III. Korean J Pharmacog 27: 47-52
  6. Reynolds J. 1989. The extra pharmacopoeia. 29th ed. The pharmaceutical press, London. p 1613-1620
  7. Jeon BH, Seong GS, Chun SG, Sung JH, Chang CC. 2005. Antioxidantive effects of white ginseng and red ginseng on liver of high fat diet-treated mice. J Ginseng Res 29: 138-144 https://doi.org/10.5142/JGR.2005.29.3.138
  8. Choi HJ, Zhang YB, An BJ, Choi C. 2002. Identification of biologically active compounds from Panax ginseng C. A. Meyer. Korean J Food Sci Technol 34: 493-497
  9. Ha DC, Ryu GH. 2005. Chemical components of red, white and extruded root ginseng. J Korean Soc Food Sci Nutr 34: 247-254 https://doi.org/10.3746/jkfn.2005.34.2.247
  10. Lee SE, Lee SU, Bang JK, Yu YJ, Seong RS. 2004. Antioxidant activities of leaf, stem, and root of Panax ginseng C. A. Meyer. Korean J Medicinal Crop Sci 12: 237- 242
  11. Kim CS, Jang DS, Che SY. 2006. Histological charateristics of Korean red ginseng in steaming processes. Korean J Medicinal Crop Sci 14: 36-40
  12. Sung KS, Chun CC, Kwon YH, Kim KH, Chang CC. 2000. Effects of red ginseng component on the antioxidative enzymes activities and lipid peroxidation in the liver of mice. J Ginseng Res 24: 29-34
  13. Sung GS, Chun SG, Chang CC. 2005. Hepatoprotective effects of white and red ginseng extracts on acetaminophen- induced hepatotoxicity in mice. J Ginseng Res 29: 131-137 https://doi.org/10.5142/JGR.2005.29.3.131
  14. Lee CK, Han YN, Kim NY, Choi JW. 2003. The therapeutic effects of Korea red ginseng on carbone tetrachloride- and galactosamine-induced hepatoxicity in rats. J Ginseng Res 27: 11-16 https://doi.org/10.5142/JGR.2003.27.1.011
  15. Kim DJ, Seug KS, Kim DW, Ko SR, Chang CC. 2004. Antioxidantive effects of red ginseng saponins on paraquot-induced oxidative stress. J Ginseng Res 28: 5-10 https://doi.org/10.5142/JGR.2004.28.1.005
  16. Sung KS, Chun C, Kwon YH, Chang CC. 2000. Effects of red ginseng component administration on glutathione and lipid peroxidation levels in mice liver. J Ginseng Res 24: 176-182
  17. Song YB, Kwak YS, Park KH, Chang SK. 2002. Effects of total saponin from red ginseng on activities of antioxidant enzymes in pregnant rats. J Ginseng Res 26: 139-144 https://doi.org/10.5142/JGR.2002.26.3.139
  18. Hwang EY, Choi SY. 2006. Quantitative analysis of phenolic compounds in different parts of Panax ginseng C. A. Meyer and its inhibitory effect on melanin biosysthesis. Korean J Medicinal Crop Sci 14: 148-152
  19. Kim KH, Sung KS, Chang CC. 2000. Effects of the antioxidative components to ginsenoside in the liver of 40-week-old mice. J Ginseng Res 24: 162-167
  20. Gelboin HV. 1980. Benzo(${\alpha}$)pyrene metabolism, activation and carcinogenesis: role and regulation of mixed-function oxidase and related enzymes. Physiol Rev 60: 1107-1166 https://doi.org/10.1152/physrev.1980.60.4.1107
  21. Ellman GL. 1959. Tissue sulfhydryl group. Arch Biochem Biophys 82: 70-72 https://doi.org/10.1016/0003-9861(59)90090-6
  22. Ohkawa H, Ohishi N, Yagi K. 1979. Assay for lipid peroxides in animal tissue by thiobarbituric acid reaction. Anal Biochem 95: 351-358 https://doi.org/10.1016/0003-2697(79)90738-3
  23. Reitman S, Frankel S. 1957. A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. Am J Clin Pathol 28: 56-63 https://doi.org/10.1093/ajcp/28.1.56
  24. Marklund S, Marklund CT. 1974. Involvement of the superoxide anion radical in the autooxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47: 469-474 https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  25. Aebi H. 1974. Catalase. In Methods of Enzymatic Analysis. Vergmeyer HU, ed. Academic press, New York, USA. Vol 2, p 673-698
  26. Paglia ED, Valentine WN. 1967. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70: 158-169
  27. Habig WH, Pabst MJ, Jakoby WB. 1974. Glutathione S-transferase: the first enzymatic step in mercapturic acid reaction. Anal Biochem 249: 7130-7139
  28. Lowry OH, Rosebrough NH, Farr AL, Randall RJ. 1951. Protein measurement with Folin phenol reagent. J Biol Chem 193: 265-275
  29. Yoon SH, Park EJ, Oh KH, Chung YG, Kwon OJ. 1993. The effect of lithospermi radix benzo($\alpha$)pyrene-induced hepatotoxicity. J Korean Soc Food Nutr 22: 144-148
  30. Cheigh HS. 1994. Lipid peroxidation and its nutritional significance. J Korean Soc Food Nutr 23: 867-871
  31. Halliwell B, Gutterridge JM. 1990. Roles of free radicals and catalytic metal ions in human disease. In Methods Enzymol. Fleischer S, Packer L, eds. Academic Press, New York, USA. Vol 186, p 1-12
  32. Kim HJ, Lee KR. 2003. Effect of Ramaria botrytis methanol extract on antioxidant enzyme activities in benzo(${\alpha}$)pyrene-treated mice. Korean J Food Sci Technol 354: 286-290
  33. Park SH, Kim JY, Chang JS, Oh EJ, Kim OM, Bae JT, Kim HJ, Hae DJ, Lee KR. 2001. Protective effect of Hericium erinaceus extracts on hepatic injury induced by benzo(${\alpha}$)pyrene in mice. J Korean Soc Food Sci Nutr 30: 928-932
  34. Bompart GJ, Prevot DS, Basacands JL. 1990. Rapid automated analysis of glutathione reductase, peroxidase and S-transferase activity: application to cisplatin induced toxicity. Clin Biochem 23: 501-504 https://doi.org/10.1016/0009-9120(90)80039-L
  35. Cohen GM, Freedman RB. 1982. Roles and functions of glutathione. Biochem Soc Trans 10: 78-85 https://doi.org/10.1042/bst0100078

Cited by

  1. Synergetic Hepatoprotective Effects of Korean Red Ginseng and Pueraria Radix on the Liver Damaged-Induced by Carbon Tetrachloride (CCl4) in Mice vol.23, pp.2, 2017, https://doi.org/10.20307/nps.2017.23.2.132
  2. 팽화홍삼분말이 벤조피렌을 투여한 마우스의 항산화 효소 활성에 미치는 영향 vol.37, pp.7, 2007, https://doi.org/10.3746/jkfn.2008.37.7.847
  3. Does Consumption of Organic Foods Contribute to Korean Consumers’ Subjective Well-Being? vol.11, pp.19, 2007, https://doi.org/10.3390/su11195496