Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
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Screening and Development of Novel Herbal Medicine for Bone Healing Treatment

골유합 촉진 치료제 개발을 위한 수종의 한약재 활성 검색

  • Lee, Jae-Dong (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-Hee University) ;
  • Baek, Yong-Hyeon (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-Hee University) ;
  • Choi, Do-Young (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-Hee University) ;
  • Huh, Jeong-Eun (Oriental Medicine Research Center for Bone & Joint Disease, Kyung-Hee University) ;
  • Yang, Ha-Ru (Oriental Medicine Research Center for Bone & Joint Disease, Kyung-Hee University) ;
  • Woo, Hyun-Su (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-Hee University) ;
  • Park, Dong-Suk (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-Hee University)
  • 이재동 (경희대학교 한의과대학 침구학교실) ;
  • 백용현 (경희대학교 한의과대학 침구학교실) ;
  • 최도영 (경희대학교 한의과대학 침구학교실) ;
  • 허정은 (경희대학교 골관절질환 한방연구센터) ;
  • 양하루 (경희대학교 골관절질환 한방연구센터) ;
  • 우현수 (경희대학교 한의과대학 침구학교실) ;
  • 박동석 (경희대학교 한의과대학 침구학교실)
  • Published : 2007.10.20
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Abstract

Objectives : The differentiation of osteoblasts is controlled by various growth factors and matrix protein expressed in bone. The aim of this study was to investigate the effects of many herbs medicine(KHBJs) for bone healing that induces osteogenic activity in human osteoblast-like SaOS-2 cells. Methods : The osteogenic effects of KHBJs were evaluated by using cell proliferation(WST-8) assay, alkaline phosphatase(ALP) activity assay, colorimetric analysis of vascular endothelial growth factor(VEGF) expression in human osteoblast like SaOS-2 cell. Also, osteogenic activity of KHBJ fractions(KHBJB and KHBJR) by activity guided fractionation were evaluated. Results : About 7 KHBJs had effect on the proliferation of osteoblast like SaOS-2 cells, and dose-dependently increased alkaline phosphatase(ALP) activity. KHBJs markedly increased expression for VEGF. Fractionated KHBJs(KHBJB or KHBJR) not enhanced more than KHBJs on osteogenic activity in SaOS-2 cells. Conclusions: This study found that 7 KHBJs had effect on proliferation, ALP activity, and VEGF expression in osteoblast like SaOS-2 cells. These results propose that KHBJs can play an important role in osteoblastic bone formation, and may possibly lead to the development of bone-forming drugs.

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