Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
권호 표지

Screening and Mechanism Study of Angiogenesis in Many Herbs Medicine

수종의 한약재에서 신생혈관형성 활성 검색 및 기전 연구

  • Huh, Jeong-Eun (Oriental Medicine Research Center for Bone & Joint Disease, Kyung-Hee University) ;
  • Baek, Yong-Hyeon (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-Hee University) ;
  • Lee, Jae-Dong (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-Hee University) ;
  • Choi, Do-Young (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-Hee University) ;
  • Park, Dong-Suk (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-Hee University)
  • 허정은 (경희대학교 골관절질환 한방연구센터) ;
  • 백용현 (경희대학교 한의과대학 침구학교실) ;
  • 이재동 (경희대학교 한의과대학 침구학교실) ;
  • 최도영 (경희대학교 한의과대학 침구학교실) ;
  • 박동석 (경희대학교 한의과대학 침구학교실)
  • Published : 2007.10.20
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : Angiogenesis consists of the proliferation, migration, and differentiation of endothelial cells, and angiogenic factors and matrix protein interactions modulate this process. The aim of this study was to determine whether herbs medicine(KHBJs) could induce angiogenic activity in human umbilical vein endothelial cells(HUVECs). Methods : The angiogenic activity of KHBJs were evaluated by proliferation using BrdU assay, chemotactic migration assay, tube formation assay, and measurement of basic fibroblast growth factor (bFGF) and vascular endothelial growth factor(VEGF) in HUVECs. Also, In order to identify enhance angiogenic activity by activity guided fractionation, the angiogenic activity of fractions of KHBJs such as KHBJB or KHBJR were evaluated in vitro and in vivo Matrigel plug angiogenesis asaay. Results : About 9 KHBJs significantly increased HUVECs proliferation in a dose-dependent manner. In addition, 9 herbs medicine(KHBJs) increased migration and tube-like formation in HUVECs. Interestingly the expression of bFGF and VEGF, an angiogenesis-inducing growth factor, were dose-dependently increased by KHBJs. However, angiogenic activity of fractionated KHBJs(KHBJB or KHBJR) not enhanced more than KHBJs in HUVECs and Matrigel plug in vivo angiogenesis assay. Conclusions : 9 KHBJs significantly induces angiogenesis in in vitro and in vivo. These results suggest that 9 KHBJs potent angiogenic agents and promising drug for the induction of neovascularization.

Keywords