Journal of Acupuncture Research

  • 제24권4호
  • /
  • Pages.157-166
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  • 2007
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  • 2586-288X(pISSN)
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  • 2586-2898(eISSN)
대한침구의학회 (Korean Acupuncture & Moxibustion Medicine Society)
권호 표지

홍화자약침의 윤활관절막내에서의 MIF활성 억제를 통한 LPS 유발 관절염의 치료 효과

The Treatment Effect of Honghwa-ja Herbal Acupuncture on Inhibitory MIF Activation on Synovial Membrane by LPS-induced Arthritis

  • 박원 (동국대학교 한의과대학 침구학교실) ;
  • 김경호 (동국대학교 한의과대학 침구학교실) ;
  • 이창환 (동국대학교 한의과대학 침구학교실) ;
  • 이동건 (동국대학교 한의과대학 침구학교실) ;
  • 이현진 (동국대학교 한의과대학 침구학교실) ;
  • 황지혜 (동국대학교 한의과대학 침구학교실) ;
  • 김갑성 (동국대학교 한의과대학 침구학교실)
  • Park, Won (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University) ;
  • Kim, Kyung-Ho (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University) ;
  • Lee, Chang-Hwan (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University) ;
  • Lee, Dong-Gun (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University) ;
  • Lee, Hyun-Jin (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University) ;
  • Hwang, Ji-Hye (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University) ;
  • Kim, Kap-Sung (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University)
  • 발행 : 2007.08.20
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초록

Objective : The aim of this study was to investigate anti-arthritic effects of Honghwa-ja herbal acupuncture extract through inhibitory MIF activation. Methods: After Rheumatoid arthritis(RA) knee joint was induced by lipopolysaccharide(LPS) in vivo, Honghwa-ja herbal acupuncture solution was applied on $ST_{35}$(犢鼻) and $EX-LE_{202}$(內膝眼) coresponding human body. To research the effect on the expression of MIF, COX-2, MMP-9 mRNA, RT-PCR was performed on LPS-stimulated Raw 264.7 cells. Results: In the Honghwa-ja herbal acupuncture solution treated Raw 264.7 cell, the mRNA expression of cytokines, RA related inflammation factors, such as the MIF, COX-2, and MMP-9 reduced concentration dependently. Positive reaction of RA-related cytokines MIF, $IL-6R-{\alpha}$, STAT3, COX-2, MMP-9 was reduced. Conclusion : Honghwa-ja herbal acupuncture extract has significant protecting ability against acute progressive RA by inhibiting the production of MIF, as a top in cytokines related to inflammation.

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