한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제35권2호
  • /
  • Pages.98-103
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  • 2007
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

계분으로부터 Lactobacillus salivarius의 분리 및 생균제적 특성

Probiotic Properties of Lactobacillus salivarius CPM-7 Isolated from Chicken Feces.

  • 임수진 (단국대학교 생명자원과학대학 동물자원학과) ;
  • 장성식 (단국대학교 생명자원과학대학 동물자원학과) ;
  • 강대경 (단국대학교 생명자원과학대학 동물자원학과)
  • Lim, Soo-Jin (Department of Animal Resources Science, School of Bio-Resources Science, Dankook University) ;
  • Jang, Sung-Sik (Department of Animal Resources Science, School of Bio-Resources Science, Dankook University) ;
  • Kang, Dae-Kyung (Department of Animal Resources Science, School of Bio-Resources Science, Dankook University)
  • 발행 : 2007.06.28
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초록

산란계의 분변으로부터 생균제 특성을 가진 유산균을 분리하기 위하여, 무작위 선발법과 agar well diffusion assay법을 사용하여 다수의 유산균을 1차 선발하였으며 이 중에서 대장균 억제능이 가장 우수한 CPM-7 균주를 분리하였다. 최종 선발된 CPM-7 균주는 형태학적 특징, 당 이용성 및 16S rRNA 서별 분석을 통하여 Lactobacillus salivarius CPM-7으로 동정되었다. L. salivarius CPM-7은 내산성 및 내담즙성이 우수한 것으로 나타났는데, pH 2에서 30분 동안 및 pH 3에서 6시간 동안 생균수가 거의 유지되었으며, bile salt 0.2%가 첨가된 MRS 배지에서 증식할 수 있었다. L. salivarius CPM-7은 a-galactosidase를 포함한 다수의 효소를 생산하는 것으로 확인되었으며, 돼지의 장 상피세포에 흡착할 수 있었다. L. salivarius CPM-7의 배양액 및 중화액은 자돈 설사를 유발하는 것으로 알려진 E. coli K88에 대한 강력한 억제능을 나타내었다.

To isolate probiotic lactic acid bacteria for animal, we have screened the microorganisms from chicken feces, by random selection and agar well diffusion assay. Among them, CPM-7 strain showing superior inhibitory activity against Escherichia coli was selected. By examining carbohydrates utilization, morphologic property and 16S rRNA gene sequence, CPM-7 strain was identified as Lactobacillus salivarius, then named L. salivarius CPM-7. L. salivarius CPM-7 produced thirteen enzymes in the test using API ZYM kit, and showed resistance to low pH and bile salts. It survived at pH 2 for 30 min. and pH 3 for 6 hr. And, it was able to grow in MRS medium containing 0.2% (w/v) bile salts. L. salivarius CPM-7 adhered to the jejunal epithelium cells of pig. Both the supernatant of L. salivarius CPM-7 and the its neutralized one showed high inhibitory activity against E. coli K88.

키워드

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