한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제35권2호
  • /
  • Pages.150-157
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  • 2007
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

유류 분해 근권세균 Rhodococcus sp. 412와 옥수수를 활용한 유류 오염 토양의 정화

Bioremediation of Oil-Contaminated Soil Using an Oil-Degrading Rhizobacterium Rhodococcus sp.412 and Zea mays.

  • 홍선화 (이화여자대학교 환경공학과) ;
  • 박혜림 (이화여자대학교 환경공학과) ;
  • 고우리 (경기과학고등학교) ;
  • 유재준 (경기과학고등학교) ;
  • 조경숙 (이화여자대학교 환경공학과)
  • Hong, Sun-Hwa (Department of Environmental Science and Engineering, Ewha Womans University) ;
  • Park, Hae-Lim (Department of Environmental Science and Engineering, Ewha Womans University) ;
  • Ko, U-Ri (Gyeonggi Science High School) ;
  • Yoo, Jae-Jun (Gyeonggi Science High School) ;
  • Cho, Kyung-Suk (Department of Environmental Science and Engineering, Ewha Womans University)
  • 발행 : 2007.06.28
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초록

디젤 오염 토양 정화를 위해 식물과 미생물의 상호관계를 활용하는 생물복원에 관한 연구를 수행하였다. 디젤을 분해하는 근권 세균인 Rhodococcus sp. 412와 디젤에 내성을 가지고 있는 식물인 옥수수(Zea mays)를 이용하여 디젤로 오염되어진 토양의 디젤 제거능과 미생물 군집변화를 조사하였다. 실험 개시 30일 후, 디젤 오염 토양에서 Rhodococcus sp. 412를 접종한 토양의 옥수수의 성장이 412균주를 접종하지 않은 토양에서의 옥수수 성장보다 약간 우수하였다. 또한 식물을 식재하거나 412균주를 접종한 토양에서 존재하는 토양에서 디젤의 잔류농도도 낮게 나타났다. 이러한 결과를 디젤 오염 토양 정화를 위해 옥수수와 Rhodococcus sp. 412를 동시에 활용하는 것이 유리함을 의미한다. 토양세균 군집 변화를 16S rDNA-PCR과 DGGE(denaturing gradient gel electrophoresis) fingerprinting 방법을 이용하여 분석하였다. 비오염 토양 시료와 디젤 오염토양 시료의 DGGE fingerprint의 유사도는 $20.8{\sim}39.3%$이었다. 또한, 비오염 토양 시료 사이의 DGGE fingerprint의 유사도는 $21.9{\sim}53.6%$, 그리고 디젤 오염 토양 시료 사이의 유사도는 $31.6{\sim}50.0%$이었다. 이러한 결과는 디젤 오염으로 인해 토양 세균 군집구조가 영향을 받았음을 시사한다.

The advanced bioremediation of diesel-contaminated soil through the exploration of bacterial interaction with plants was studied. A diesel-degrading rhizobacterium, Rhodococcus sp.412, and a plant species, Zea mays, having tolerant against diesel was selected. Zea mays was seeded in uncontaminated soil or diesel-contaminated soil with or without Rhodococcus sp. 412. After cultivating for 30 days, the growth of Zea mays in the contaminated soil inoculated with Rhodococcus sp. 412 was better than that in the contaminated soil without the bacterium. The residual diesel concentrations were lowered by seeding Zea mays or inoculating Rhodococctis sp. 412. These results Indicate that the simultaneous use of Zea mays and Rhodococcus sp. 412 can give beneficial effect to the remediation of oil-contaminated soil. Bacterial community was characterized using a 16S rDNA PCR and denaturing gradient gel electrophoresis (DGGE) fingerprinting method. The similarities of DGGE fingerprints were $20.8{\sim}39.9%$ between the uncontaminated soil and diesel contaminated soil. The similarities of DGGE fingerprints were $21.9%{\sim}53.6%$ between the uncontaminated soil samples, and $31.6%{\sim}50.0%$ between the diesel-contaminated soil samples. This results indicated that the structure of bacterial community was significantly influence by diesel contamination.

키워드

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