Antioxidant and Antiproliferative Activities of Methanol Extracts from Leafy Vegetables Consumed in Korea

  • Kim, Hoi-Kyung (Department of Food Science and Technology, Chungbuk National University) ;
  • Bang, Chan-Sook (Department of Food Science and Technology, Chungbuk National University) ;
  • Choi, Young-Min (Department of Food Science and Technology, Chungbuk National University) ;
  • Lee, Jun-Soo (Department of Food Science and Technology, Chungbuk National University)
  • Published : 2007.10.31

Abstract

There has been growing interest in the antioxidative and anticarcinogenic effects of vegetables. This study was aimed at evaluating the antioxidative and antiproliferative activities of 13 leafy vegetables consumed in Korea. New-beet and ornamental kale contained >1,000 mg of polyphenolics/100g fresh weight (FW), which was the greatest amount among the test vegetable extracts. Ornamental kale also contained the greatest amount (232.84 mg/100g FW) of flavonoids. With the exception of chicory, values of 1.1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging activities showed similar trends. New-beet was found to be the greatest antioxidant among the test vegetable extracts. New-beet exhibited the highest antiproliferative activity (>60%) against all tumor cells. No relationship was found between antiproliferative activity and antioxidant contents or antioxidant activities among samples.

Keywords

References

  1. Doll R. An overview of the epidemiological evidence linking diet and cancer. P. Nutr. Soc. 49: 119-131 (1990) https://doi.org/10.1079/PNS19900018
  2. Bravo L. Polyphenols: Chemistry, dietary sources, metabolism, and nutritional significance. Nutr. Rev. 56: 317-333 (1998) https://doi.org/10.1111/j.1753-4887.1998.tb01670.x
  3. Croft KD. The chemistry and biological effects of flavonoids and phenolics acids. Ann. NY Acad. Sci. 854: 435-442 (1998) https://doi.org/10.1111/j.1749-6632.1998.tb09922.x
  4. Ross JA, Kasum CM. Dietary flavonoids: Bioavailability, metabolic effects, and safety. Annu. Rev. Nutr. 22: 19-34 (2002) https://doi.org/10.1146/annurev.nutr.22.111401.144957
  5. Duthie G, Crozier A. Plant-derived phenolics antioxidants. Curr. Opin. Lipidol. 11: 43-47 (2000) https://doi.org/10.1097/00041433-200002000-00007
  6. Chu YH, Chang CL, Hsu HF. Flavonoid content of several vegetables and their antioxidant activity. J. Sci. Food Agr. 80: 561- 566 (2000) https://doi.org/10.1002/(SICI)1097-0010(200004)80:5<561::AID-JSFA574>3.0.CO;2-#
  7. Fukumoto LR, Mazza G. Assessing antioxidant and prooxidant activities of phenolics compounds. J. Agr. Food Chem. 48: 3597- 3604 (2000) https://doi.org/10.1021/jf000220w
  8. Unno T, Sugimoto A, Kakuda T. Scavenging effect of tea catechins and their epimers on superoxideanion radicals generated by a hypoxanthine and xanthine oxidase system. J. Sci. Food Agr. 80: 601-606 (2000) https://doi.org/10.1002/(SICI)1097-0010(200004)80:5<601::AID-JSFA581>3.0.CO;2-O
  9. Noroozi M, Angerson WJ, Lean MEJ. Effects of flavonoids and vitamin C on oxidative DNA damage to human lymphocytes. Am. J. Clin. Nutr. 67: 1210-1218 (1998) https://doi.org/10.1093/ajcn/67.6.1210
  10. Kawaii S, Tomono Y, Katase E, Ogawa K, Yano M. Antiproliferative activity of flavonoids on several cancer cell lines. Biosci. Biotech. Bioch. 63: 896-899 (1999) https://doi.org/10.1271/bbb.63.896
  11. Manthey JA, Guthrie N. Antiproliferative activities of citrus flavonoids against six human cancer cell lines. J. Agr. Food Chem. 50: 5837- 5843 (2002) https://doi.org/10.1021/jf020121d
  12. Miranda CL, Stevens JF, Helmrich A, Henderson MC, Rodriguez RJ, Yang Y-H, Deinzer ML, Barnes DW, Buhler DR. Antiproliferative and cytotoxic effects of prenylated flavonoids from hops (Humulus lupulus) in human cancer cell lines. Food Chem. Toxicol. 37: 271-285 (1999) https://doi.org/10.1016/S0278-6915(99)00019-8
  13. Wenzel U, Kuntz S, Brendel MD, Daniel H. Dietary flavone is a potent apoptosis inducer in human colon carcinoma cells. Cancer Res. 60: 3823-3831 (2000)
  14. Singleton VL, Rossi JA Jr. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Viticult. 16: 144-158 (1965)
  15. Maria INM, Maria II, Antonio RS, Marta AV. Comparison of the free radical-scavenging activity of propolis from several region of Argentina. J. Ethnopharmacol. 71: 109-114 (2000) https://doi.org/10.1016/S0378-8741(99)00189-0
  16. Kim DO, Lee KW, Lee HJ, Lee CY. Vitamin C equivalent antioxidant capacity (VCEAC) of phenolic phytochemicals. J. Agr. Food Chem. 50: 3713-3717 (2002) https://doi.org/10.1021/jf020071c
  17. Leong LP, Shui G. An investigation of antioxidant capacity of fruits in Singapore markets. Food Chem. 76: 69-75 (2002) https://doi.org/10.1016/S0308-8146(01)00251-5
  18. Robert R, Nicoletta P, Anna P, Ananth P, Min Y, Catherine RE. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio. Med. 26: 1231-1237 (1999) https://doi.org/10.1016/S0891-5849(98)00315-3
  19. Forni LG, Mora-Arellano VO, Packer JE, Willson RL. Nitrogen dioxide and related free radicals: Electron-transfer reactions with organic compounds in solutions containing nitrite or nitrate. J. Chem. Soc. Perk. T. 2: 1-6 (1986)
  20. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 65: 55-63 (1983) https://doi.org/10.1016/0022-1759(83)90303-4
  21. Choi Y, Ku JB, Chang HB, Lee J. Antioxidant activities and total phenolics of ethanol extracts from several edible mushrooms produced in Korea. Food Sci. Biotechnol. 14: 700-703 (2005)
  22. Yoo KM, Kim DO, Lee CY. Evaluation of different methods of antioxidant measurement. Food Sci. Biotechnol. 16: 177-182 (2007)
  23. Crozier A, Lean MEJ, Mcdonald MS, Black C. Quantitative analysis of the flavonoid content of commercial tomatoes, onions, lettuce, and celery. J. Agr. Food Chem. 45: 590-595 (1997) https://doi.org/10.1021/jf960339y
  24. Heimler D, Vignolini P, Dini MG, Vincieri FF, Romani A. Antiradical activity and polyphenol composition of local Brassicaceae edible varieties. Food Chem. 99: 464-469 (2006) https://doi.org/10.1016/j.foodchem.2005.07.057
  25. Huang Z, Wang B, Eaves DH, Shikany JM, Pace RD. Phenolic compound profile of selected vegetables frequently consumed by African Americans in the southeast United States. Food Chem. 103: 1395-1402 (2007) https://doi.org/10.1016/j.foodchem.2006.10.077