Changes in the Antioxidant Activity of Onion (Allium cepa) Extracts with Heat Treatment

  • Woo, Koan-Sik (Department of Food Science and Technology, Chungbuk National University) ;
  • Hwang, In-Guk (Department of Food Science and Technology, Chungbuk National University) ;
  • Kim, Tae-Myoung (Laboratory of Cancer Prevention, College of Veterinary Medicine, Chungbuk National University) ;
  • Kim, Dae-Joong (Laboratory of Cancer Prevention, College of Veterinary Medicine, Chungbuk National University) ;
  • Hong, Jin-Tae (College of Pharmacy, Chungbuk National University) ;
  • Jeong, Heon-Sang (Department of Food Science and Technology, Chungbuk National University)
  • Published : 2007.10.31

Abstract

We evaluated the effects of heat treatment on various properties of onion extracts (Allium cepa). Onion was heated at various temperatures ($110-150^{\circ}C$) for various times (1-5 hr), and the total polyphenol, flavonoid, and free sugar contents, and antioxidant activity were investigated. With increased heating temperatures and exposure times, the total contents of polyphenols and flavonoids, as well as antioxidant activity increased. The highest total polyphenol content (189.80 mg/100g) occurred after heating for 2 hr at $140^{\circ}C$. The highest total flavonoid content (252.51 mg/100g) occurred after heating for 3 hr at $150^{\circ}C$. The antioxidant activity assessed using the DPPH method was highest, at 2.1 mg/mL ($IC_{50}$ value), after heating for 2 hr at $150^{\circ}C$. Correlations between antioxidant activity and the total polyphenol, total flavonoid, and fructose contents were highly significant (all p<0.01). The optimal heating time and temperature were 2 hr and $130^{\circ}C$.

Keywords

References

  1. Kim SJ, Kim GH. Quantification of quercetin in different parts of onion and its DPPH radical scavenging and antibacterial activity. Food Sci. Biotechnol. 15: 39-43 (2006)
  2. Lee JH, Kim SD, Lee JY, Kim KN, Kim HS. Analysis of flavonoids in concentrated pomegranate extracts by HPLC with diode array detection. Food Sci. Biotechnol. 14: 171-174 (2005)
  3. Saleheen D, Ali SA, Yasinzai M. Antileishmanial activity of aqueous onion extract in vitro. Fitoterapia 75: 9-13 (2004) https://doi.org/10.1016/j.fitote.2003.07.010
  4. Block E, Naganathan S, Putman D, Zhao SH. Allium chemistry: HPLC analysis of thiosulfonates from onion, garlic, wild garlic, leek, scallion, shallot, elephant garlic, and Chinese chive. Uniquely high allyl to methyl ratios in some garlic samples. J. Agr. Food Chem. 40: 2418-2430 (1992) https://doi.org/10.1021/jf00024a017
  5. Whitemore BB, Naidu AS. Thiosulfinates. pp. 265-380. In: Natural Food Antimicrobial Systems. Naidu AS (ed). CRC Press, Boca Raton, FL, USA. (2000)
  6. Ibarz A, Pagán J, Garza S. Kinetic models for color changes in pear puree during heating at relatively high temperatures. J. Engin. 39: 415-422 (1999)
  7. Dewanto V, Wu X, Adom KK, Liu RH. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J. Agr. Food Chem. 50: 3010-3014 (2002) https://doi.org/10.1021/jf0115589
  8. Kim WY, Kim JM, Han SB, Lee SK, Kim ND, Park MK. Steaming of ginseng at high temperature enhances biological activity. J. Natur. Prod. 63: 1702-1704 (2000) https://doi.org/10.1021/np990152b
  9. Yang SJ, Woo KS, Yoo JS, Kang TS, Noh YH, Lee J, Jeong HS. Change of Korean ginseng components with high temperature and pressure treatment. Korean J. Food Sci. Technol. 38: 521-525 (2006)
  10. Woo KS, Jang KI, Kim KY, Lee HB, Jeong HS. Antioxidative activity of heat treated licorice (Glycyrrhiza uralensis Fisch) extracts. Korean J. Food Sci. Technol. 38: 355-360 (2006)
  11. Kwon OC, Woo KS, Kim TM, Kim DJ, Hong JT, Jeong HS. Physicochemical characteristics of garlic (Allium sativum L.) on the high temperature and pressure treatment. Korean J. Food Sci. Technol. 38: 331-336 (2006)
  12. Hwang IG, Woo KS, Kim TM, Kim DJ, Yang MH, Jeong HS. Change of physicochemical characteristics of Korean pear (Pyrus pyrifolia Nakai) juice with heat treatment conditions. Korean J. Food Sci. Technol. 38: 342-347 (2006)
  13. Choi Y, Lee SM, Chun J, Lee HB, Lee J. Influence of heat treatment on the antioxidant activities and polyphenolic compounds of shiitake (Lentinus edodes) mushroom. Food Chem. 99: 381-387 (2006) https://doi.org/10.1016/j.foodchem.2005.08.004
  14. Mokbel MS, Hashinaga F. Evaluation of the antioxidant activity of extracts from buntan (Citrus grandis Osbeck) fruit tissues. Food Chem. 94: 529-534 (2006) https://doi.org/10.1016/j.foodchem.2004.11.042
  15. Jia Z, Tang M, Wu J. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64: 555-559 (1999) https://doi.org/10.1016/S0308-8146(98)00102-2
  16. Bae SK, Lee YC, Kim HW. The browning reaction and inhibition on apple concentrated juice. J. Korean Soc. Food Sci. Nutr. 30: 6-13 (2001)
  17. Tepe B, Sokmen M, Akpulat HA, Sokmen A. Screening of the antioxidant potentials of six Salvia species from Turkey. Food Chem. 95: 200-204 (2006) https://doi.org/10.1016/j.foodchem.2004.12.031
  18. Lim HK, Yoo ES, Moon JY, Jeon YJ, Cho SK. Antioxidant activity of extracts from dangyuja (Citrus grandis Osbeck) fruits produced in Jeju island. Food Sci. Biotechnol. 15: 312-316 (2006)
  19. Dewanto V, Xianzhong W, Liu RH. Processed sweet corn has higher antioxidant activity. J. Agr. Food Chem. 50: 4959-4964 (2002) https://doi.org/10.1021/jf0255937
  20. Stewart AJ, Bozonnet S, Mullen W, Jenkins GI, Michael EJ, Crozier A. Occurrence of flavonols in tomatoes and tomato-based products. J. Agr. Food Chem. 48: 2663-2669 (2000) https://doi.org/10.1021/jf000070p
  21. Jeong SM, Kim SY, Kim DR, Jo SC, Nam KC, Ahn DU, Lee SC. Effect of heat treatment on the antioxidant activity of extracts from citrus peels. J. Agr. Food Chem. 52: 3389-3393 (2004) https://doi.org/10.1021/jf049899k
  22. Kim MJ, Kim CY, Park I. Prevention of enzymatic browning of pear by onion extract. Food Chem. 89: 181-184 (2005) https://doi.org/10.1016/j.foodchem.2004.02.018
  23. Velioglu YS, Mazza G, Gao L, Oomah BD. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J. Agr. Food Chem. 46: 4113-4117 (1998) https://doi.org/10.1021/jf9801973
  24. Manzocco L, Calligaris S, Mastrocola D, Nicoli MC, Lerici CR. Review of non-enzymatic browning and antioxidant capacity in processed foods. Trends Food Sci. Tech. 11: 340-346 (2001) https://doi.org/10.1016/S0924-2244(01)00014-0
  25. Nicoli MC, Anese M, Parpinel M. Influence of processing on the antioxidant properties of fruit and vegetables. Trends Food Sci. Tech. 10: 94-100 (1999) https://doi.org/10.1016/S0924-2244(99)00023-0