Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Factors Affecting Agrobacterium tumefaciens-mediated Transformation of Panax ginseng C.A. Meyer

  • Kim, Ok-Tae (Division of Ginseng and Medicinal Crops, National Institute of Crop Sciences, RDA) ;
  • Jung, Su-Jin (Division of Ginseng and Medicinal Crops, National Institute of Crop Sciences, RDA) ;
  • Bang, Kyong-Hwan (Division of Ginseng and Medicinal Crops, National Institute of Crop Sciences, RDA) ;
  • Kim, Young-Chang (Division of Ginseng and Medicinal Crops, National Institute of Crop Sciences, RDA) ;
  • Shin, Yu-Su (Division of Ginseng and Medicinal Crops, National Institute of Crop Sciences, RDA) ;
  • Sung, Jung-Sook (Division of Ginseng and Medicinal Crops, National Institute of Crop Sciences, RDA) ;
  • Park, Chun-Geon (Division of Ginseng and Medicinal Crops, National Institute of Crop Sciences, RDA) ;
  • Seong, Nak-Sul (Division of Ginseng and Medicinal Crops, National Institute of Crop Sciences, RDA) ;
  • Cha, Seon-Woo (Division of Ginseng and Medicinal Crops, National Institute of Crop Sciences, RDA) ;
  • Park, Hee-Woon (Division of Ginseng and Medicinal Crops, National Institute of Crop Sciences, RDA)
  • Published : 2007.04.30
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Abstract

A protocol for the production of transgenic Panax ginseng C.A. Meyer was established via Agrobacterium tumefaciens-mediated genetic transformation of direct somatic embryos. A number of conditions related to the co-cultivation were tested with respect to maximizing transformation efficiency. The results showed that pH of the co-cultivation medium (5.7), the bacterial growth phase (optical density; $OD_{600}$ = 0.8), co-cultivation period (3 days), and acetosyringone concentration $(100\;{\mu}M)$ had positive effects on transformation. Selected plantlets were cultured on the medium at an elevated hygromycin level(30 mg/l). Integration of the transgenes into the P. ginseng nuclear genome was confirmed by PCR analysis using hpt primers and by Southern hybridization using hpt-specific probe. The transgenic plantlets were obtained after 3-month cultivation and did not show any detectable variation in morphology or growth characteristics compared to wild-type plants.

Keywords

References

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