Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Neuroprotective Effect of Korean Mistletoe Extract against Damage Induced by Hydrogen Peroxide in Cultured Rat Cortical Neurons

  • Lee, Ju-Hyun (College of Veterinary Medicine and Research Institute of herbal Medicine, Chungbuk National University) ;
  • Cho, Soon-Ock (College of Veterinary Medicine and Research Institute of herbal Medicine, Chungbuk National University) ;
  • Ban, Ju-Yeon (College of Veterinary Medicine and Research Institute of herbal Medicine, Chungbuk National University) ;
  • Song, Kyung-Sik (College of Agriculture and Life-Sciences, Kyungpook National University) ;
  • Seong, Yeon-Hee (College of Veterinary Medicine and Research Institute of herbal Medicine, Chungbuk National University)
  • Published : 2007.04.30
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Abstract

The protective effect of ethanol extract of Korean mistletoe (KM; Viscum album coloratum) on hydrogen peroxide $(H_{2}O_{2})-induced$ neurotoxicity was examined in primary cultured rat cortical neurons. $H_{2}O_{2}$ reduced viability of cortical neurons in a concentration-dependent manner. The addition of KM, over a concentration range of 10 to 100 ${\mu}g/ml$, concentration-dependently prevented the $H_{2}O_{2}(100\;{\mu}M)-induced$ neuronal cell death, as assessed by a 3-[4,5-dimethylthiazol-2-yl]-2,5-di-phenyl-tetrazolium bromide (MTT) assay and Hoechst 33342 staining. KM significantly inhibited $H_{2}O_{2}-induced$ elevation of the cytosolic $Ca^{2+}$ concentration $([Ca^{2+}]_{c})$, which was measured by a fluorescent dye, fluo-4 AM. KM inhibited glutamate release into medium and generation of reactive oxygen species (ROS) induced by $H_{2}O_{2}$. These results suggest that KM may mitigate the $H_{2}O_{2}-induced$ neurotoxiciy by interfering with the increase of $[Ca^{2+}]_{c}$, and inhibiting glutamate release and generation of ROS in cultured neurons.

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References

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