Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Anticariogenic Activity and Glucosyltransferase Inhibitory Effects of Extracts from Pine Needle and Twig

솔잎 및 소나무 가지 추출물의 항충치 활성 및 glucosyltransferase 억제 효과

  • Published : 2007.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

To investigate anticariogenic effects of extracts from pine needles and twigs, we measured the extracts' antimicrobial activities against Streptococcus mutans, their glucosyltransferase (GTase) inhibitory effects and their stabilities under various conditions of heat and pH. The 70% ethanol extracts of pine needles and twigs showed greater antimicrobial activities against S. mutans than the hot water extracts, and the pine twig extracts showed greater antimicrobial activity than the pine needle extracts. More specifically, the n-hexane fractions of the pine needles and twigs had the greatest effects against S. mutans, and their minimal inhibition concentration (MIC) was 0.6 mg/mL. The results of the extracts' GTase inhibitory effects indicated that the 70% ethanol extracts had greater effects than hot water extracts, and the pine twig extracts showed greater effects than the pine needle extracts. The antimicrobial activity against S. mutans for the pine needle n-hexane traction was greatly reduced as temperature increased, and was also largely affected by changes in pH. These results suggest that extracts from pine needles and twigs have anticariogenic effects and could be used as anticariogenic food additives.

솔잎과 소나무 가지의 항충치 효과와 기능성 소재로서의 활용 가능성을 조사하기 위하여 추출물과 용매 분획의 S. mutans에 대한 항균효과, GTase 활성 억제효과 그리고 열과 pH 에 대한 안정성을 측정하였다. 열수추출물 보다는 70% EtOH 추출물이, 솔잎보다는 가지 추출물이 S. mutans에 대한 생육저해 효과가 큰 것으로 나타났으며, 특히 n-hexane 분획의 MIC 는 0.6 mg/mL로서 S. mutans에 대해 가장 높은 항균효과를 나타내었다. 그리고 솔잎과 가지 추출물의 GTase 활성 억제 효과는 S. mutans에 대한 생육억제 효과 결과와 마찬가지로 열수추출물 보다는 70% EtOH 추출물이, 솔잎 보다는 가지 추출물이 GTase 활성 억제 효과가 큰 것으로 나타났다. 한편 솔잎의 n-hexane은 열처리 온도가 증가함에 따라 S. mutans에 대한 항균력이 크게 감소하였고 pH의 변화에도 크게 영향받는 것으로 나타났다.

Keywords

References

  1. Hamada S, Ooshima T, Torii M, Imanishi H, Masuda N, Mizuno J, Sobue S, Kotani S. Dental caries induction in experimental animals by clinical strains of Streptococcus mutans isolated from Japanese children. Microbiol. Immunol. 22: 301-314 (1978) https://doi.org/10.1111/j.1348-0421.1978.tb00375.x
  2. Mosci F, Perito S, Bassa S, Capuano A. The role of Streptococcus mutans in human caries. Minerva Stomatol. 39: 413-429 (1990)
  3. Gibbons RJ, Van Houte J. On the formation dental plaque. J. Periodontol. 44: 347-360 (1973) https://doi.org/10.1902/jop.1973.44.6.347
  4. McGhee JR, Michalek SM. Immunology of dental caries: microbial aspects and local immunity. Ann. Rev. Microbiol. 135: 595-638 (1981)
  5. You YS, Park KM, Kim YB. Antimicrobial activity of some medical herbs and species against Streptococcus mutans. Korean J. Appl. Microbiol. Biotechnol. 21: 187-191 (1993)
  6. Choi IW, Jung CH, Park YK. Anticariogenic activities of various plant extracts. Korean J. Food Sci. Technol. 35: 1221-1225 (2003)
  7. Park YM, Kim SJ, Jo KH, Yang EJ, Jung ST. Anticariogenic and antioxidant activities from medical herbs. J. Korean Soc. Food Sci. Nutr. 35: 284-293 (2006) https://doi.org/10.3746/jkfn.2006.35.3.284
  8. Lee YS, Park HJ, You JS, Park HH, Kwon lB, Lee HY. Isolation of an anticariogenic compound from Magnoliae bark. Korean J. Food Sci. Technol. 30: 230-236 (1998)
  9. Jang GH, Ahn BY, Oh SH, Choi DS, Kwon YJ. Anticariogenic effects of Coptis chinensis Franch extract. Korean J. Food Sci. Technol. 32: 1396-1402 (2000)
  10. Kim SJ, Park YM, Jung ST. Anticariogenic effects and inhibition of glucosyltransferase activity of Chrysanthemun indicum L. extracts. Korean J. Food Culture 20: 341-345 (2005)
  11. Yoon SY, Kim SH, Chung HL, Lee JJ, Huh CS, Baek YJ. Anticariogenic effects of unripe apple extract. Korean J. Food Sci. Technol. 32: 168-173 (2000)
  12. Hwang JK, Shim JS, Baek NI, Pyun YR. Xanthorrhizol: a potential antibacterial agent from Curcuma xanthorrhiza against Streptococcus mutans. Planta Med. 66: 196-197 (2000) https://doi.org/10.1055/s-0029-1243135
  13. Endo A, Hayashida O, Murakawa S. Mutastein: A new inhibitor of adhesive-insoluble glucan synthesis by glucosyltransferases of Streptococcus mutans. J. Antibiot. 36: 203-207 (1983) https://doi.org/10.7164/antibiotics.36.203
  14. Hattori M, Kusumoto IT, Namba T, Ishigami T, Hara Y. Effect of tea polyphenols on glucan synthesis by glucosyl transferase from Streptococcus mutans. Chem. Pharm. Bull. 38: 717-720 (1990) https://doi.org/10.1248/cpb.38.717
  15. Boo YC, Jeon CO, Oh JY. Isolation of 4-hydroxy-5-methyl3[2H]-furanone from pine needles as an antioxidative. Agric. Chem. Biotechnol. 37: 310-314 (1994)
  16. Kim SM, Cho YS, Sung SK, Lee IG; Lee SH, Kim DG. Antioxidative and nitrite scavenging activity of pine needle and green tea extracts. Korean J. Food Sci. Anim. Resour. 22: 13-19 (2002)
  17. Choi HS, Hang D, Kang SC, Sohn ES, Lee SP, Pyo SK, Son EW. Immunomodulatory activity of pine needle (Pinus densiflora) extracts in macrophages. J Food Sci. Nutr. 11: 105-109 (2006) https://doi.org/10.3746/jfn.2006.11.2.105
  18. Jeon JR, Kim JY, Lee KM, Cho DH. Anti-obese effects of mixture contained pine needle, black tea, and green tea extracts. J. Korean Soc. Appl. BioI. Chem. 48: 375-381 (2005)
  19. Kim JD, Yoon TH, Choi M, Im KJ, Ju JS, Lee SY. Effect of dietary supplementation with pine leaf on lipid parameters in rat. Korean J. Gerontol. 1: 47-50 (1990)
  20. Choi JH, Kim DI, Park SH, Back SJ, Kim NJ, Cho WK, Kim KJ, Kim HS. Effects of pine needle ethyl acetate fraction on acetylcholine (ACh) and its related enzymes in brain of rats. Korean J. Nutr. 37: 95-99 (2004)
  21. Choi MY, Choi EJ, Lee E, Rhim TJ, Cha BC, Park HJ. Antimicrobial activities of pine needle (Pinus densiflora Sieb et Zucc.) extract. Korean J. Appl. Mirobiol. Biotechnol. 25: 293-297 (1997)
  22. Kim YS, Shin DH. Volatile components and antibacterial effects of pine needle (Pinus densiflora S. et Z.) extracts. Food MicrobioI. 22: 37-45 (2005) https://doi.org/10.1016/j.fm.2004.05.002
  23. Park CS. Antibacterial activity of ethanol extract of pine needle against pathogenic bacteria. Korean J. Postharvest Sci. Technol. 5: 380-385 (1998)
  24. Park KN, Lee SH. Antimicrobial activity of pine needle extract and horseradish on the growth of Vibrio. J. Korean Soc. Food Sci. Nutr. 32: 185-190 (2003) https://doi.org/10.3746/jkfn.2003.32.2.185
  25. Kuk JH, Ma SJ, Park KH. Isolation and characterization of benzoic acid with antimicrobial activity from needle of Pinus densiflora. Korean J. Food Sci. Technol. 29: 204-210 (1997)
  26. Lim CM, Kyung KH, Yoo YI. Antimicrobial effects of butylated hydroxyanisole (BRA) and butylated hydroxytoluene (BHT). Korean J. Food Sci. Technol. 19: 54-60 (1987)
  27. Conner DE, Beuchat LR. Effect of essential oil from plants on growth of food spoilage yeasts. J. Food Sci. 49: 429-434 (1984) https://doi.org/10.1111/j.1365-2621.1984.tb12437.x
  28. Miyoshi M, Imoto T, Kasagi T. Antieurodontic effect of various fractions extracted from the leaves of Gymnema sylvestre. J. Yonago Med. Ass. 38: 127-137 (1987)
  29. SAS Institute, Inc. SAS User's guide. Statistical Analysis Systems Institute, Cary, NC, USA (1993)
  30. Nakahara K, Kawabata S, Ono H, Ogura K, Tanaka T, Ooshima T, Hamada S. Inhibitory effect of oolong tea polyphenols on glucosyltransferases of mutans Streptococci. Appl. Environ. Microb. 59: 968-973 (1993)
  31. Matsumoto M, Hamada S, Ooshima T. Molecular analysis of the inhibitory effects of oolong tea polyphenols on glucan-binding domain of recombinant glucosyltransferases from Streptococcus mutans MT8148. FEMS Microbiol. Lett. 228: 73-80 (2003) https://doi.org/10.1016/S0378-1097(03)00723-7
  32. Jerez M, Pinelo M, Sineiro J, Nunez Ml Influence of extraction conditions on phenolic yields from pine bark: assessment of procyanidins polymerization degree by thiolysis. Food Chem. 94: 406-414 (2006) https://doi.org/10.1016/j.foodchem.2004.11.036
  33. Rohr GE, Meier B, Sticher O. Analysis of procyanidins. Stud. Nat. Prod. Chem. 21: 497-570 (2000) https://doi.org/10.1016/S1572-5995(00)80013-7
  34. Cho JE, Lee MJ, Lee YB, Yoon JR. Comparisons of volatile compounds of Pinus densiflora on kinds of extraction solvent and parts of pinus. J. Korean Soc. Food Sci. Nutr. 28: 973-979 (1999)
  35. Kim YS, Shin DH. Volatile components and antibacterial effects of pine needle (Pinus densiflora S. et Z.) extracts. Food MicrobioI. 22: 37-45 (2005) https://doi.org/10.1016/j.fm.2004.05.002
  36. Burt S. Essential oils: their antimicrobial properties and potential applications in food-a review. Int. J. Food Microbiol. 94: 223-253 (2004) https://doi.org/10.1016/j.ijfoodmicro.2004.03.022
  37. Oussalah M, Caillet S, Saucier L, Lacroix M. Inhibitory effects of selected plant essential oils on the growth of four pathogenic bacteria: E. coli O157:H7, Salmonella typhimurium, Staphylococcus aureus and Listeria monocytogenes. Food Control 18: 414420 (2007) https://doi.org/10.1016/j.foodcont.2005.11.009