Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Effects of Lentinus edodes Extract on the Loperamide-induced Constipation in Rats

표고버섯 추출액이 loperamide로 유도된 변비에 미치는 영향

  • Jin, Young-Gun (Department of Veterinary Medicine, Cheju National University) ;
  • Kim, Dong-Gun (Department of Veterinary Medicine, Cheju National University) ;
  • Jin, Ju-Yeon (Department of Veterinary Medicine, Cheju National University) ;
  • Lee, Young-Jae (Department of Veterinary Medicine, Cheju National University) ;
  • Park, Min-Kyung (Department of Human Nutrition and Food Science, Chungwoon University)
  • Published : 2007.02.28
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Abstract

The preventive effect of Lentinus edodes extract (LE) against constipation was studied in rats. Rats were pretreated with LE contained in drinking water at the concentration of 10%, 20% and 40% over 30 days. Constipation was induced by subcutaneous injection of loperamide (4 mg/kg/day) 3 days prior to sacrifice. Treatment of loperamide resulted in decreases in the number and wet weight of fecal pellets, and increase in the number of fecal pellet in the distal colon and cecocolon weight. In contrast, the number and wet weight of fecal pellets were increased, and the number of fecal pellet in the distal colon and the cecocolon weight were decreased in LE-pretreated groups compared to the loperamide-treated group. Blood parameters such as white blood cell, red blood cell, hemoglobin, hematocrit, platelet, mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration, and serum alanine transaminase, aspartate aminotransferase and alkaline phosphatase activities, and blood urea nitrogen and creatinine values were not significantly different between the groups. In addition, LE (0.5 mg/mL) increased spontaneous contractile activity, which was reduced by atropine or loperamide in isolated rat ileum. Theses results suggest that the improvement of constipation symptoms in LE-pretreated rats resulted from a stimulatory effect of LE on intestine contractile activity.

표고버섯 추출액을 10, 20 및 40%로 30일간 음용수에 섞어 실험동물에 투여하고 실험 28-30일에 loperamide(4 mg/kg/day, s.c.)로 변비를 유도한 뒤 표고버섯 추출액의 변비 예방효과를 측정하였다. Loperamide를 단독 투여한 군은 정상 대조군과 비교하여 변의 개수 및 중량이 유의적으로 감소하였으며 원위 결장 내 변잔류의 증가 및 cecocolonic segment의 무게가 증가하였다. 표고버섯 추출액 및 loperamide를 투여한 군은 loperamide를 단독 투여한 군과 비교하여 변의 개수 및 중량이 유의적으로 증가하였으며 원위 결장 내 잔류 변 및 cecocolonic segment의 무게도 감소하였다. 이러한 결과는 표고버섯 추출액이 in vivo에서 변비 개선효과가 있음을 보여준다. 변의 수분함량은 실험군 간의 유의한 차이가 없었지만 loperamide에 의한 변비 유발 시 감소하는 경향이 있었으며, 표고버섯 추출액 투여군에서는 변의 수분함량 감소가 다소 완화되었다. 한편, 혈 중 WBC, RBC, HGB, HCT, PLT, MCV, MCH, MCHC치 및 혈청 ALT, AST, ALP, BUN 및 creatinine 수치는 정상 대조군, loperamide 처리군 및 표고버섯 추출액 처리군 사이에서 차이가 없었다. In vitro 실험결과, 회장 적출 절편에 표고버섯 추출액 동결건조물(0.5 mg/mL)을 전 처리 시 loperamide에 의한 장력과 진폭 억제가 부분적으로 차단되었으며, atropine에 의해 유도된 진폭의 억제도 효과적으로 차단되었다. 이러한 결과는 표고버섯 추출물의 변비 개선효과가 장의 운동성 촉진과 관련이 있음을 시사한다.

Keywords

References

  1. Lee GD, Chang HG, Kim HK. Antioxidative and nitrite-scavenging activities of edible mushrooms. Korean J. Food Sci, Technol. 29: 432-436 (1997)
  2. Hwang KH, Kim HK, Han YN. Inhibitory activity of edible mushrooms on the tissue thromboplastin (tissue factor). Korean J. Food Sci. Technol. 29: 161-166 (1997)
  3. Choi MY, Lim SS, Chung TY. The effects of hot water soluble polysaccharides from Lentinus edodes on lipid metabolism in the rats fed butter yellow. J. Korean Soc. Food Sci. Nutr. 29: 294-299 (2000)
  4. Song JY, Yoon KJ, Yoon HK, Koo SJ. Effects of ${\beta}$-glucan from Lentinus edodes and Hordeum vulgare on blood glucose and lipid composition in alloxan-induced diebetic mice. Korean J. Food Sci. Technol. 33: 802-807 (2001)
  5. Lee BW, Park KM. Anti-tumor activity of protein-bound polysaccharides extracted from mycelia of Lentinus edodes. Korean J. Food Sci. Technol. 30: 665-671 (1998)
  6. Park JM, Lee SH, Kim JO, Park HJ, Park JB, Sin JI. In vitro and in vivo effects of extracts of Lentinus edodes on tumor growth in human papillomavirus 16 oncogenes-transformed animal tumor model. Korean J. Food Sci. Technol. 36: 141-146 (2004)
  7. Hwang YJ, Nam HK, Chang MJ, Noh GW, Kim SH. Effect of Lentinus edodes and Pleurotus eryngii extracts on proliferation and apoptosis in human colon cancer cell lines. Korean J. Food Sci. Technol. 32: 217-222 (2003) https://doi.org/10.3746/jkfn.2003.32.2.217
  8. Chihara G. Immune modulation agents and their mechanisms(lentinan, a T celloriented immunopotentiator). Basel 19: 409-436 (1985)
  9. Han MJ, Bae EA, Rhee YK, Kim DH. Effect of mushrooms on the growth of intestinal lactic acid bacteria. Korean J. Food Sci. Technol. 28: 947-952 (1996)
  10. Han MJ, Bae EA, Rhee YK, Kim DH. Effect of Lentinus edodes on some enzymes of intestinal bacteria. Korean J. Food Sci. Technol. 33: 142-145 (2001)
  11. Locke GR III. The epidemiology of functional disorders in North America, Gastroenterol. Clin. North Am. 25: 1-19 (1996) https://doi.org/10.1016/S0889-8553(05)70362-9
  12. Lembo A, Camilleri M. Chronic constipation. New Engl. J. Med. 349: 1360-1348 (2003) https://doi.org/10.1056/NEJMra020995
  13. Rao SSC. Dyssynergic defecation. Disorders of the anorectum. Gastroenterol. Clin. North Am. 31: 97-114 (2001)
  14. Siproudhis L, Pigot F, Godberge P, Damon H, Soudan D, Bigard MA. Defecation disorders: a french population survey. Dis. Colon. Rectum 49: 219-227 (2006) https://doi.org/10.1007/s10350-005-0249-8
  15. Watanabe T, Nakaya N, Kurashima K, Kuriyama S, Tsubono Y, Tsuji I. Constipation, laxative use and risk of colorectal cancer: the Miyagi cohort study. Eur. J. Cancer 40: 2109-2115 (2004) https://doi.org/10.1016/j.ejca.2004.06.014
  16. Cummings JH. Constipation, dietary fiber and the control of large bowel function. Postgrad. Med. J. 60: 811-819 (1984) https://doi.org/10.1136/pgmj.60.709.811
  17. Muller-Lissner SA, Kamm MA, Scarpinato C, Wald A. Myths and misconceptions about chronic constipation. Am. J. Gastroenterol. 100: 232-242 (2005) https://doi.org/10.1111/j.1572-0241.2005.40885.x
  18. Bingham SA, Cummings JH. Effect of exercise and physical fitness on large intestinal function. Gastroenterology 97: 1389-1399 (1989) https://doi.org/10.1016/0016-5085(89)90381-8
  19. Smith B, Effect of irritant purgatives on the myenteric plexus in man and the mouse. Gut 9: 139-143 (1968) https://doi.org/10.1136/gut.9.2.139
  20. Smith B. Pathologic changes in the colon produced by anthraquinone laxatives. Dis. Colon. Rectum 16: 455-458 (1973) https://doi.org/10.1007/BF02588868
  21. Everhart JE, Go VLW, Johannes RS, Fitzsimmons SC, Roth HP, White LR. A longitudinal survey of self-reported bowel habits in the US. Dig. Dis. Sci. 34: 1153-1162 (1989) https://doi.org/10.1007/BF01537261
  22. Shimotoyodome A, Meguro S, Hase T, Tokimitsu I, Sakata T. Decreased colonic mucus in rats with loperamide-induced constipation. Comp. Biochem, Physiol. 126: 203-211 (2000) https://doi.org/10.1016/S1095-6433(00)00194-X
  23. Nakamura T, Agata G, Mizutani M, Iino H. Effects of brewer's yeast cell wall on constipation and defecation in experimentally constipated rats. Biosci. Biotech. Bioch. 65: 774-780 (2001) https://doi.org/10.1271/bbb.65.774
  24. Baily CA, Gibson RM, Kubena LF, Huff WE, Harvey RB, Ochratoxin A and dietary protein. 2. Effects on hematology and various clinical chemistry measurements. Poult. Sci. 68: 1664-1671 (1989) https://doi.org/10.3382/ps.0681664
  25. Mok LL, Cooper CW, Thompson JC. Relaxation of rat gastrointestinal smooth muscle by parathyroid hormone. J. Bone Miner. Res. 2: 329-336 (1987) https://doi.org/10.1002/jbmr.5650020410
  26. Schiller LR, Santa Ana CA, Moravski SG, Fordtran JS, Mechanism of the antidiarrheal effect of loperamide. Gastroenterology 86: 1475-1480 (1984)
  27. Theodoru V, Fioramonti J, Hachet T, Bueno L. Absorptive and motor components of the antidiarrheal action of loperamide: an in vivo study in pigs. Gut 11:1355-1359 (1991)
  28. Read NW. Speculations on the role of motility in the pathogenesis and treatment of diarrhoea J. Gastroenterol. suppl. 84: 45-63 (1983)
  29. Heel RC, Brogden RN, Speight TM, Avery GS. Loperamide: a review of its pharmacological properties and therapeutic efficacy in diarrhoea. Drugs 15: 33-52 (1978)
  30. Awouters F, Megens A, Verlinden M, Schuurkes J. Niemegeers C, Jaassen PAJ. Loperamide, survey of studies on mechanism of its antidiarrheal activity. Dig. Dis. Sci. 38: 977-995 (1993) https://doi.org/10.1007/BF01295711
  31. Burleigh DE. Opioid and non-opioid actions of loperamide on cholinergic nerve function in human isolated colon. Eur, J. Pharmacol. 152: 39-46 (1988) https://doi.org/10.1016/0014-2999(88)90833-3
  32. Stoll R, Ruppin H, Domschke W. Calmodulin-mediated effects of loperamide on chloride transport by brush border membrane vesicles from human ileum. Gastroenterology 95: 69-76 (1988) https://doi.org/10.1016/0016-5085(88)90292-2