The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Production of Mycelia and Water Soluble Polysaccharides from Submerged Culture of Lentinus lepideus in Bioreactor

생물반응기를 이용한 잣버섯(Lentinus lepideus)의 균사체 및 수용성 다당체 생산특성

  • Ahn, Jin-Kwon (Div. of Biotechnology, Korea Forest Research Institute) ;
  • Ka, Kang-Hyeon (Div. of Wood Chemistry & Microbiology, Korea Forest Research Institute) ;
  • Lee, Wi-Young (Div. of Biotechnology, Korea Forest Research Institute)
  • 안진권 (국립산림과학원 생물공학과) ;
  • 가강현 (국립산림과학원 화학미생물과) ;
  • 이위영 (국립산림과학원 생물공학과)
  • Published : 2007.06.30
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Abstract

The mushroom Lentinus lepideus was used to produce mycelial as well as soluble polysaccharides in bioreactor cultures. To determine optimal submerged culture conditions, both growth characteristics and water soluble polysaccharides production were compared among four different types of bioreactor and culture conditions. For the production of mycelial biomass, the following bioreactors were proven to be effective in decreasing order: an external-loop type air-lift bioreactor (ETAB; 7g/l), a balloon type air bubble bioreactor (BTBB; 6.2g/l), a stirrer type bioreactor (STB; 6g/l), and a column type air bubble bioreactor (CTBB; 5g/l). Maxiaml production of water soluble exopolysaccharides (EPS; 0.62g/l) and endopolysaccharides (PPS; 7.7%) could also be obtained from BTBB. The mycelial biomass increased with increase in glucose concentration from 15g/l to 75g/l in the media. In contrast, PPS contents in the cells decreased with increase in glucose concentration in the media, showing the highest PPS content (7%) at 15g/l. Among different medium feeding types, fed-batch culture based on concentration control in media (10g/l) produced higher mycelia than fed-batch culture based on volume control of media (5.8g/l) or batch culture (3.4g/l). EPS production was also higher in fed-batch culture based on medium concentration control than that in other feeding types.

잣버섯 균사체 배양에 적합한 생물반응기는 풍선형의 공기부양식의 생물반응기가, 수용성 다당체의 생산에도 생장이 좋았던 풍선형 공기부양식 생물반응기가 유리한 것으로 나타났다. 배지의 탄소원 농도별에 따른 수용성 세포외 다당체의 생산량은 농도 처리간 차이가 없었으나 수용성 세포내 다당체는 탄소원 농도가 낮은 즉 C/N율이 낮을수록 증가하였다. 잣버섯 균사체는 고농도의 배지 배양조건에서 생물반응기내로 배지 공급형태가 배지량 증가방법보다 배지농도 조절에 의한 연속 배양방법이 우수한 것으로 나타났다.

Keywords

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