The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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A Natural Fungus-derived Elicitor for Induction of Systemic Acquired Resistance (SAR) in Potato

진균 세포벽 유래 신물질을 이용한 감자의 전신적 획득저항성 유도

  • Park, Hae-Jun (Biodreams Co. Ltd.) ;
  • Kim, Hong-Gi (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
  • 박해준 ((주)바이오드림스) ;
  • 김홍기 (충남대학교 농업생명과학대학 응용생물학과)
  • Published : 2007.06.30
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Abstract

It was investigated that systemic acquired resistance (SAR) was induced in plant treated with a elicitor, which was derived from a non-virulent fungus. The elicitor, a hyphal cell wall component derived from fungus, induced a production of phytoalexin and a generation of reactive oxygen species (ROS) in potato treated with its low level concentrations. The effect of the fungus-derived elicitor was better than that of virulent pathogen-derived elicitor, which was well known in potato. These results, therefore, suggested potentcial use of fungus-derived elicitor as a new plant protector for commercial development.

식물에 대해 병원성균이 아닌 자연계에서 존재하는 진균류로 부터 세포벽 성분을 분리하여 식물의 유도 저항성을 유도하는 엘리시터로써의 가능성을 시험하였다. 그 결과 선발된 진균 세포벽 성분이 감자에서 종래 알려진 어떤 엘리시터 보다도 아주 낮은 농도처리에서도 활성산소와 파이토알렉신 생성을 다량 유도하였다. 뿐 만 아니라 감자괴경을 이용한 병저항성 생물검정에서도 우수한 유도저항성이 발현되었음을 확인하였다. 그러므로 자연에서 분리된 일반적인 균주의 세포벽물질 엘리시터도 식물체의 유도저항성을 효과적으로 유도할 수 있는 새로운 식물병방제제로서의 개발 가능성이 있음을 시사하였다.

Keywords

References

  1. Baker, C. J., Orlandi, E. W. and Mock, N. M. 1993. Harpin, an elicitor of the hypersensitive response in tobacco caused by Erwinia amylovora, elicits active oxygen production in suspension cell. Plant Physiol. 102: 1341-1344 https://doi.org/10.1104/pp.102.4.1341
  2. Brisson, L. F., Tehaken, R. and Lamb, C. J. 1994. Function of oxidative cross-linking of cell wall structural proteins in plant disease resistance. Plant Cell 6: 1703-1712 https://doi.org/10.1105/tpc.6.12.1703
  3. Chai, H. B. and Doke, N. Superoxide anion generation; A response of potato leaves to infection with Phytophthora infestans. Phytopathology 77: 645-649
  4. Doke, N. 1983a. Involvement of superoxide anion generation in the hypersensitive response of potato tuber tissues to infection with an incompatible race of Phytophthora infestans and to the hyphal wall components. Physiol. Plant Pathol. 23: 345-357 https://doi.org/10.1016/0048-4059(83)90019-X
  5. Doke, N, 1983b. Generation of superoxide anion by potato tuber protoplasts upon the hypersensitive response to hyphal wall components of Phytophthora infestans and specific inhibition of the reaction by suppressors of hypersensitivity. Physiol. Plant Pathol. 23: 359-367 https://doi.org/10.1016/0048-4059(83)90020-6
  6. Doke, N, 1985. NADPH-dependent $O_2^-$ generation in membrane fraction isolated from wounded potato tubers inoculated with Phytophthora infestans. Physiol. Plant Pathol. 27: 311-322 https://doi.org/10.1016/0048-4059(85)90044-X
  7. Doke, N. and Tomiyama, K. 1980. Effect of hyphal with components from Phytophthora infestans on protoplasts of potato tuber tissues. Physiol. Plant Pathol. 16: 169-176 https://doi.org/10.1016/0048-4059(80)90031-4
  8. Doke, N. and Miura, Y. 1995. In vitro activation of NADPH-dependent $O_2^-$ generating system in a plasma membrane-rich fraction of potato tuber tissues by treatment with an elicitor from Phytophthora infestans or with digitonin. Physiol. Mol. Plant Pathol. 46: 17-28 https://doi.org/10.1006/pmpp.1995.1002
  9. Doke, N., Miura, Y., Sanchez, L. M., Park, H.-J., Noritake, T., Yoshioka, H. and Kawakita, K. 1996. The oxidative burst protects plants against pathogen attack: mechanism and role as an emergency signal for plant bio-defence. Gene 179: 45-51 https://doi.org/10.1016/S0378-1119(96)00423-4
  10. Jabs, T., Tschope, M., Colling, C., Hahlbrock, K. and Scheel, D. 1997. Elicitor-stimulated ion fluxes and $O_2^-$ from the oxidative burst are essential components in triggering defense gene activation and phytoalexin synthesis in parsley. Proc. Natl. Acad. Sci. USA 94: 4800-4805
  11. Kamoum, S., van West, P., Vleeshouwers, V. G A. A., de Groot, K. E. and Groveers, F. 1998. resistance of Nicothiana benthamiana to Phytophthora infestans is mediated by the recognition of the elicitor protein INF 1. Plant Cell 10: 1413-1425 https://doi.org/10.1105/tpc.10.9.1413
  12. Mehdy, M. C. 1994. Active oxygen species in plant defense against pathogens. Plant Physiol. 105: 467-472 https://doi.org/10.1104/pp.105.2.467
  13. Miura, Y., Yoshioka, H. and Doke, N. 1995. An autophotographic determination of the active oxygen generation in potato tuber discs during hypersensitive response to fungal infection or elicitor. Plant Sci. 105: 45-52 https://doi.org/10.1016/0168-9452(94)04040-N
  14. Noritake, T., Kawakita, K. and Doke, N. 1996. Nitric oxide induces phytoalexin accumulation in potato tuber tissues. Plant Cell Physiol. 37: 113-116 https://doi.org/10.1093/oxfordjournals.pcp.a028908
  15. Park, H.-J., Miura, Y., Kawakita, K., Yoshioka, H. and Doke, N. 1998. Physiological mechanisms of a sub-systemic oxidative burst triggered by elicitor-induced local oxidative burst in potato tuber slices. Plant Cell Physiol. 39: 1218-1225 https://doi.org/10.1093/oxfordjournals.pcp.a029323
  16. Park, K. S., Ahn, I. P. and Kim, C. H. 2001. systemic resistance and expression of the pathogenesis related genes mediated by the plant growth-promoting rhizobacterium Bacillus amyloliquefaciens EXTN-1 against anthracnose disease in cucumber. Mycobiology 29: 48-53
  17. Price, A. H., Taylor, A., Ripley, S. J., Griffiths, A. Trewavas, A. J. and Knght, M. R. 1994. Oxidative signals in tobacco increase cytosolic calcium. Plant Cell 6: 1301-1310 https://doi.org/10.1105/tpc.6.9.1301
  18. Sakia, R., Yadav, M., Varghese, S., Singh, B. P., Gogoi, D. K., Kumar, R. and Arora, D. K. 2006. Role of Riboflavin in induced resistance agianst Fusarium wilt and Charcoal rot diseases of Chickpea. Plant Pathol. J. 22: 339-347 https://doi.org/10.5423/PPJ.2006.22.4.339
  19. von Loon, L. C., Bakker, P. A. H. M. and Pieterse, C. M. J. 1998. Systemic resistance induced by rhizosphere bacteria. Annu. Rev. Phytopathol. 36: 453-483 https://doi.org/10.1146/annurev.phyto.36.1.453
  20. Yoshioka, H., Miyabe, M., Hayakawa, Y. and Doke, N. 1996. Expression of gene for phenylalanine ammonia-lyase and 3hydroxy-3-methylglutaryl CoA reductase in aged potato tubers infected with Phytophthora irfestans. Plant Cell Physiol. 37: 81-90 https://doi.org/10.1093/oxfordjournals.pcp.a028917
  21. Yoshioka, H., Sugie, K., Park, H,-J., Maeda, H., Tsuda, N., Kwakita, K. and Doke, N. 2001. Induction of plant gp91 phox homolog by fungal cell wall, arachidonic acid and salicylic acid in potato. Mol. Plant-Microbe Interact. 14: 725-736 https://doi.org/10.1094/MPMI.2001.14.6.725