PCR based Detection of Helicobacter spp. in Saliva, Dental plaque, Vomitus and Feces of Dogs

  • Ha, Sung-Woo (College of Veterinary Medicine and BK 21 program for Veterinary Science, Seoul National University) ;
  • Yoo, Jong-Hyun (BK21 Program of Integrative network systems for veterinarians in basic science, industrial animals and preventive medicine, Konkuk University) ;
  • Chung, Tae-Ho (College of Veterinary Medicine and BK 21 program for Veterinary Science, Seoul National University) ;
  • Jung, Woo-Sung (College of Veterinary Medicine and BK 21 program for Veterinary Science, Seoul National University) ;
  • Youn, Hwa-Young (College of Veterinary Medicine and BK 21 program for Veterinary Science, Seoul National University) ;
  • Chae, Joon-Seok (College of Veterinary Medicine and BK 21 program for Veterinary Science, Seoul National University) ;
  • Hwang, Cheol-Yong (College of Veterinary Medicine and BK 21 program for Veterinary Science, Seoul National University)
  • 발행 : 2008.12.31

초록

This study was aimed to determine the prevalence of Helicobacter spp. in privately owned pet dog's oral cavity samples (saliva, dental plaque, vomitus) and fecal samples in Korea and to evaluate the potential route for transmission. Total 100 patients dogs attending one Veterinary Medical Teaching Hospital were examined by Helicobacter genus-specific PCR assay and these dogs were divided into two groups whether they had gastrointestinal signs (vomiting, nausea and diarrhea) or not. The total detection rate of Helicobacter spp. by PCR in saliva, dental plaque and fecal samples was 23%, 1% and 68% respectively. The difference of prevalence with regarding the gastrointestinal sings was not significant. In vomitus, two of seven samples had positive results. These results suggested that Helicobacter spp. are present in the oral cavity although they were present in very low number and are not like to be normal oral flora of the oral cavity and Helicobacter spp. in dogs could be transmitted through oral-oral, gastrooral and fecal-oral route.

키워드

참고문헌

  1. Alejandra B, Marianella P, Maria C, Maria EC, Claudio T, Rosa G, Vicente L. Detection of Helicobacter pylori DNA in the oral cavity and gastroduodenal system of a Venezuelan population. J Med Microbiol 2002; 51: 764-770 https://doi.org/10.1099/0022-1317-51-9-764
  2. Allaker RP, Young KA, Hardie JM, Domizio P, Meadows NJ. Prevalence of Helicobacter pylori at oral and gastrointestinal sites in children Evidence for possible oral-to-oral transmission. J Med Microbiol 2002; 51: 312-317 https://doi.org/10.1099/0022-1317-51-4-312
  3. Bronsdon MA, Goodwin CS, Sly LI, Chilvers T, Schoenknecht FD. Helicobacter nemestrinae sp. Nov., a spiral bacterium found in the stomach of a pig tailed macaque (Macaca nemestrina). Int J Syst Bacteril 1991; 41: 148-153 https://doi.org/10.1099/00207713-41-1-148
  4. Chuanfu LI, Tuanzuh HA, Donald A, Ferguson DA, Chi DS, Zhao R, Patel NR, Krishnaswamy G, Tomas E. A newly developed PCR assay of H. pylori in gastric biopsy, saliva, and feces; Evidence of high prevalence of H. pylori in saliva supports oral transmission. Dig Dis Sci 1996; 41: 2142-2149 https://doi.org/10.1007/BF02071393
  5. Dieterich C, Wiesel P, Neiger R, Blum A, Corthesy-Theulaz I. Presence of multiple "Helicobacter heilmannii" strains in an individual suffering from ulcers and in his two cats. J Clin Microbiol 1998; 36: 1366-1370
  6. Eaton KA, Dewhirst FE, Radin MJ, Fox JG, Paster BJ, Krakowka S, Morgan DR. Helicobacter acnonyx sp. Nov., isolated from cheetahs with gastritis. Int J Syst Bacteriol 1993; 43: 99-106 https://doi.org/10.1099/00207713-43-1-99
  7. Eaton KA, Dewhirst FE, Paster BJ, Tzellas N, Coleman, B. E., Paola, J., and Sherding, R. Prevalence and varieties of Helicobacter species in dogs from random sources and pet dogs: Animal and public health implications. J Clin Microbiol 1996; 34; 3165-3170
  8. Fox JG, Taylor NS, Edmonds P, Brenner DJ. Campylobacter pylori subs. Mustelae sub. Nov. isolated from the gastric mucosa of ferrets (Mustela putorious furo), and an emended description of Campylobacter pylori. Int J Syst Bacteriol 1988; 38: 367-370 https://doi.org/10.1099/00207713-38-4-367
  9. Fox JG, Batcheder M, Marini R, Yan L, Handt L, Li X, Shames B, Hayward A, Campbell J, Murphy JC. Helicobacter pylori-induced gastritis in the domestic cat. Infect Immun 1995; 63: 2674-2680
  10. Geyer C, Colbatzky F, Lechner J, Hermanns W. Occurrence of spiral-shaped bacteria in gastric biopsies of dogs and cats. Vet Rec 1993; 133: 18-19 https://doi.org/10.1136/vr.133.1.18
  11. Goosen G, Theron J, Ntsala M, Maree FF, Olckers A, Botha SJ, Lastovica AJ and van der Merwe SW. Evaluation of novel heminested PCR assay based on the phosphoglucosamine mutase gene for detection of Helico-bacter pylori in saliva and dental plaque. J Clin Microbiol 2002; 40: 205-209 https://doi.org/10.1128/JCM.40.1.205-209.2002
  12. Handt LK, Fox JG, Dewhirst FE, Fraser GJ, Paster BJ, Yan, LL, Rozmiarek H, Rufo R, Stalis IH. Helicobacter pylori isolated from domestic cat: public health implications. Infect Immun 1994; 62: 2367-2374
  13. Harper CM, Dangler CA, Xu S, Feng Y, Shen, Z, Sheppard B, Stamper A, Dewhirst FE, Paster BJ, Fox JG. Isolation and characterization of Helicobacter sp. from the gastric mucosa of dolphins, lagenorhynchus actus and delphinus delphis. Appl Environ Microbiol 2000; 66, 4751- 4757 https://doi.org/10.1128/AEM.66.11.4751-4757.2000
  14. Happonen I, Saari S, Catern L, Tyni O, Hanninen ML, Westermark E.Occurrence and topographical mapping of gastric Helicobacter-like organisms and their association with histological changes in apparently healthy dogs and cats. J Vet Med Asso 1996; 43: 305-315 https://doi.org/10.1111/j.1439-0442.1996.tb00457.x
  15. Heilmann KL, Borchard F. Gastritis due to spiral shaped bacteria other than H. pylori: clinical, hostological, and ultrastructural findings. Gut 1991; 32: 137-140 https://doi.org/10.1136/gut.32.2.137
  16. Hermanns W, Kregel K, Breuer W, Lechner J. Helicobacter- like organisms: Histological examination of gastric biopsies from dogs and cats. J Comp Pathol 1995; 112: 307-318 https://doi.org/10.1016/S0021-9975(05)80083-0
  17. Hwang CY, Han HR, Youn HY. Development of noninvasive fecal PCR assay for detecting the Helicobacter species infection in dogs. J. Vet Clin 2002; 19: 295-298
  18. Hwang CY, Han HR, Youn HY. Prevalence and clinical characterization of gastric Helicobacter species infection of dogs and cats in Korea. J Vet Sci 2002; 3: 123-133
  19. Lavelle JP, Landas S, Mitros FA, Conklin JL. Acute gastritis associated with spiral organisms from cats Dig Dis Sci 1994; 39: 744-750 https://doi.org/10.1007/BF02087417
  20. Lee A, Karakowka S, Fox JG, Otto G, Eaton KA, Murphy JC. Role of Helicobacter felis on chronic canine gastritis. Vet Pathol 1992; 29: 487-494 https://doi.org/10.1177/030098589202900601
  21. Megenegatti M, Holton J, Figura N, Biasco G, Ricci G, Oderda G, Conte R, Miglioli M, Vaira D. Clinical significance of Helicobacter pylori seropositivity and seronegativity in asymptomatic blood donors. Dig Dis Sci 1998; 43: 2542- 2548 https://doi.org/10.1023/A:1026663022278
  22. Parsonnet J, Friedman GD, Vandersten DP, Chang Y, Vogelman JH, Orentreich N, Sibley RK. Helicobacter pylori infection and the risk of gastric calcinoma. N Eng J Med 1991; 325: 1127-1231 https://doi.org/10.1056/NEJM199110173251603
  23. Queiroz DMM, Rocha GA, Mendes EN, Moura SB, Oliveira AMR, Miranda D. Association between Helicobacter and gastric ulcers disease of pars esophagea in swine. Gastroenterology 1996; 111: 19-27 https://doi.org/10.1053/gast.1996.v111.pm8698198
  24. Riggio MP, Lennon A. Identification by PCR of Helicobacter pylori in subgingival plaque of adult periodontitis patients. J Med Microbiol 1999; 48: 317-322 https://doi.org/10.1099/00222615-48-3-317
  25. Shames B, Krajden S, Fuksa M, Babida C, Penner JL. Evidence for the occurrence of the same strain of Campylobacter pylori in the stomach and dental plaque. J Clin Microbial 1989; 27: 2849-2850
  26. Stolte M, Wallens E, Bethke B, Ritter M, Eidt H. Helicobacter heilmannii ( formerly Gastrospirillum hominis ) gastritis : an infection transmitted by animals? Scand J Gastroenterol. 1994; 29: 1061-1064 https://doi.org/10.3109/00365529409094888
  27. Thomas JE, Gibson GR, Darboe MK, Dale A, Weaver LT. Isolation of Helicobacter pylori from human faeces. Lancet 1992; 2: 1194-1195
  28. Tomson MA, Storey P, Greer R. Cleghorn GJ. Caninehuman transmission of Gastrospillium hominis. Lancet 1994; 343: 1605-1607 https://doi.org/10.1016/S0140-6736(94)93060-0
  29. Uemura N, Okamoto S, Yamamoto S, Matsumura N, Yamaguchi S, Yamakido M. Helicobacter pylori infection and the development of cancer. N Eng J Med 2001; 345: 784-789 https://doi.org/10.1056/NEJMoa001999
  30. Umeda M, Kobayashi H, Takeuchi Y, Hayashi J, Morotome- Hayashi Y, Yano K, Aoki A, Ohkusa T, Ishikawa I. High prevalence of Helicobacter pylori detected by PCR in the oral cavities of periodontitis patients. J Periodontol 2003; 74: 129-134 https://doi.org/10.1902/jop.2003.74.1.129
  31. Weber AF, Hasa O, Sautter JH. Some observations concerning the presence of spirilla in the fundic glands of dogs and cats. Am J Vet Res 1958; 19: 677-680
  32. Yamasaki K, Suematsu H and Takahashi T. Comparison of gastric lesion in dogs and cats with and without gastric spiral organism. J Am Vet Med Assoc 1998; 212: 529-533