Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Inhibitory Effects of Naringenin, Kaempherol, and Apigenin on Cholesterol Biosynthesis in HepG2 and MCF-7 Cells

  • Kim, Kee-Tae (Bio/Molecular Informatics Center, Konkuk University) ;
  • Yeo, Eun-Ju (Division of Animal Life Science, Konkuk University) ;
  • Moon, Sun-Hee (Division of Animal Life Science, Konkuk University) ;
  • Cho, Ssang-Goo (Bio/Molecular Informatics Center, Konkuk University) ;
  • Han, Ye-Sun (Bio/Molecular Informatics Center, Konkuk University) ;
  • Nah, Seung-Yeol (Bio/Molecular Informatics Center, Konkuk University) ;
  • Paik, Hyun-Dong (Bio/Molecular Informatics Center, Konkuk University)
  • Published : 2008.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

The inhibitory effects of naringenin, kaempherol, and apigenin on the production of cholesterol in HepG2 KCLB 88065 and MCF-7 KCLB 30022 cells were evaluated. In this study, quercetin was used as a reference reagent. After incubation for 3 days, fat-soluble contents of both cell types were extracted by using the Folch method and the cholesterol contents in both cultured cells were determined by high performance liquid chromatography. The concentration of cholesterol in untreated each tissue cells was $12.2{\pm}0.11$ and $8.83{\pm}0.12\;mg/g$ of lipid, respectively. The total concentration of each flavonoid was adjusted to 0, 35, or $350{\mu}M$ in the culture broth. As the results, the addition of 2% methanol and dimethyl sulfoxide (DMSO) to the media (control for flavonoid solvents) did not significantly affect cell growth; however, DMSO caused an increase in the production of cholesterol. Each flavonoid inhibited the production of cholesterol in both HepG2 and MCF-7 cells at the concentration of $35{\mu}M$ above. In addition, the inhibitory effect of kaempherol on the production of cholesterol in these cells was greater than the other flavonoids tested and HepG2 cells are more sensitive to flavonoids than MCF-7. From the results, the inhibitory effects of flavonoids on cholesterol production are different depending on the cell type.

Keywords

References

  1. Wald NJ, Law MR. Serum cholesterol and ischemic heart disease. Atherosclerosis 118: S1-S5 (1995) https://doi.org/10.1016/0021-9150(95)05587-M
  2. Ha CG, Cho JK, Lee CH, Chai YC, Ha YA, Shin SH. Cholesterol lowering effect of Lactobacillus plantarum isolated from human feces. J. Microbiol. Biotechn. 16: 1201-1209 (2006)
  3. Lee HW, Chien JT, Chen BH. Inhibition of cholesterol oxidation in marinated foods as affected by antioxidants during heating. Food Chem. 108: 234-244 (2008) https://doi.org/10.1016/j.foodchem.2007.10.072
  4. Chien JT, Hsu DJ, Chen BH. Kinetic model for studying the effect of quercetin on cholesterol oxidation during heating. J. Agr. Food Chem. 54: 1486-1492 (2006) https://doi.org/10.1021/jf052529r
  5. Gla${\beta}$er G, Graefe EU, Struck F, Veit M, Gebhardt R. Comparison of antioxidative capacities and inhibitory effects on cholesterol biosynthesis of quercetin and potential metabolites. Phytomedicine 9: 33-40 (2002) https://doi.org/10.1078/0944-7113-00080
  6. Lee JY, Moon SO, Kwon YJ, Rhee SJ, Park HR, Choi SW. Identification and quantification of anthocyanins and flavonoids in mulberry cultivars. Food Sci. Biotechnol. 13: 176-184 (2004)
  7. Choi YM, Ku JB, Chang HB, Lee J. Antioxidant activities and total phenolics of ethanol extracts from several edible mushrooms produced in Korea. Food Sci. Biotechnol. 14: 700-703 (2005)
  8. Morre DM, Morre DJ. Anticancer activity of grape and grape skin extracts alone and combined with green tea infusions. Cancer Lett. 238: 202-209 (2006) https://doi.org/10.1016/j.canlet.2005.07.011
  9. Laurence G, Nadine L, Denis B. Role of the cyclic AMP-dependent pathway in free radical-induced cholesterol accumulation in vascular smooth muscle cells. Free Radical Bio. Med. 29: 181-190 (2000) https://doi.org/10.1016/S0891-5849(00)00337-3
  10. Funatsu T, Suzuki K, Goto M, Arai Y, Kakuta H, Tanaka H, Yasuda S, Ida M, Nishijima S, Miyata K. Prolonged inhibition of cholesterol synthesis by atorvastatin inhibits apoB-100 and triglyceride secretion from HepG2 cells. Atherosclerosis 157: 107-115 (2001) https://doi.org/10.1016/S0021-9150(00)00714-0
  11. Miki I, Teruo M. The growth inhibitory effect of conjugated linoleic acid on a human hepatoma cell line, HepG2, is induced by a change in fatty acid metabolism, but not the facilitation of lipid peroxidation in the cells. Biochim. Biophys. Acta 1530: 162-171 (2001) https://doi.org/10.1016/S1388-1981(00)00180-3
  12. Lee YH, Ho JN, Dong MS, Park CH, Kim HK, Hong BS, Shin DH, Cho HY. Transfected HepG2 cells for evaluation of catechin effects on alcohol-induced CYP2E1 cytotoxicity. J. Microbiol. Biotechn. 15: 1310-1316 (2005)
  13. Suleen SH, Sebely P. Margarine phytosterols decrease the secretion of atherogenic lipoproteins from HepG2 liver and Caco2 intestinal cells. Atherosclerosis 182: 29-36 (2005) https://doi.org/10.1016/j.atherosclerosis.2005.01.031
  14. Eugenios K, Pau. BA. Novel HPLC analysis of tocopherols, tocotrienols, and cholesterol in tissue. Free Radical Bio. Med. 27: 1137-1140 (1999) https://doi.org/10.1016/S0891-5849(99)00205-1
  15. Pazzucconi F, Dorigotti F, Gianfranceschi G, Campagnoli G, Champagnoli G, Sirtori M, Franceschini G, Sirtori CR. Therapy with HMG CoA reductase inhibitors: Characteristics of the long-term permanence of hypocholesterolemic activity. Atherosclerosis 117: 189-198 (1995) https://doi.org/10.1016/0021-9150(95)05571-D
  16. Stein JH, Keevil JG, Wiebe DA, Aaschlimann S, Folts JD. Purple grape juice improves endothelial function and reduces the susceptibility of LDL cholesterol to oxidation in patients with coronary disease. Circulation 100: 1050-1055 (1999) https://doi.org/10.1161/01.CIR.100.10.1050