Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Isolation of Cysteine Proteinase Gene (PgCysP1) from Panax ginseng and Response of This Gene to Abiotic Stresses

인삼으로부터 Cysteine Proteinase 유전자의 분리 및 환경 스트레스에 대한 반응

  • Jeong, Dae-Young (Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Kim, Yu-Jin (Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Shim, Ju-Sun (Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Lee, Jung-Hye (Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • In, Jun-Gyo (Biopia Co., Ltd.) ;
  • Lee, Bum-Soo (Biopia Co., Ltd.) ;
  • Yang, Deok-Chun (Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University)
  • 정대영 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행) ;
  • 김유진 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행) ;
  • 심주선 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행) ;
  • 이정혜 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행) ;
  • 인준교 ((주)바이오피아) ;
  • 이범수 ((주)바이오피아) ;
  • 양덕춘 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행)
  • Published : 2008.12.31
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Abstract

Cysteine proteinases play an essential role in plant growth and development but also in senescence and programmed cell death. They participate in both anabolic and catabolic processes. In addition, they are involved in signalling pathways and in the response to biotic and abiotic stresses. A cDNA clone encoding cysteine proteinase (CP) gene, designated PgCysP1, was isolated from Panax ginseng C. A. Meyer. Reverse transcriptase (RT)-PCR results showed that PgCysP1 expressed at different level in P. ginseng hairy root. Different stresses such as biotic as well as abiotic stresses triggered a significant induction of PgCysP1. The positive responses of PgCysP1 to the various stimuli suggested that PgCysP1 may help to protect the plant against reactive environmental stresses.

14년생 인삼의 뿌리로부터 cDNA library를 제작한 후 무작위로 뽑은 EST clone 중에서 cysteine proteinase(CP) 유전자에 높은 상동성을 나타내는 clone 6개를 선발하였고 이를 바탕으로 PgCysP1을 제작하였다. 인삼의 CP 유전자는 전장의 길이가 1,398bp로 366개의 아미노산을 코딩하는 1,101bp의 ORF를 가지고 있다. PgCysP1의 아미노산 서열과 이차구조를 분석한 결과, 기존에 보고된 식물들과 높은 상동성을 나타내었으며 PgCysP1는 papain family에 속하는 것으로 확인되었다. RT-PCR 분석결과, 인삼의 PgCysP1는 NaCl, 저온, wound, salicylic acid에 대해 그 발현 양상이 상동성을 나타내는 다른식물의 CP 유전자 발현과 유사한 양성을 보였고, 이에 PgCysP1은 CP gene으로 사료되며, 앞으로 인삼 식물체의 환경 스트레스에 대한 내성 연구가 요구된다.

Keywords

References

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